Extended Amplification<i>In Vitro</i>and Replicative Senescence: Key Factors Implicated in the Success of Human Myoblast Transplantation

Cooper, Racquel N.; Thiesson, D.; Furling, Denis; Santo, James P. Di; Butler-Browne, Gillian; Mouly, Vincent

doi:10.1089/104303403322168000

Cited by 41 publications

(32 citation statements)

References 51 publications

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“…The number of human fiber profiles is higher than that obtained with bona fide human satellite cells presenting already a high regenerative capacity. 42 However, these satellite cells were isolated from a very young donor and have been extensively expanded after their isolation. Therefore, we also assessed the regenerative potential of CD133 + cells as compared to satellite cells freshly isolated from a 15-year-old donor, which would match the age range of the CD133 + cell donors: the regenerative potential of the 15-year-old donor primary culture was even lower than that of the control satellite cells isolated from the 5-day-old infant muscle, and therefore much lower than that of CD133 + cells.…”

Section: Discussionmentioning

confidence: 99%

In Vivo Myogenic Potential of Human CD133+ Muscle-derived Stem Cells: A Quantitative Study

et al. 2009

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In recent years, numerous reports have identified in mouse different sources of myogenic cells distinct from satellite cells that exhibited a variable myogenic potential in vivo. Myogenic stem cells have also been described in humans, although their regenerative potential has rarely been quantified. In this study, we have investigated the myogenic potential of human muscle-derived cells based on the expression of the stem cell marker CD133 as compared to bona fide satellite cells already used in clinical trials. The efficiency of these cells to participate in muscle regeneration and contribute to the renewal of the satellite cell pool, when injected intramuscularly, has been evaluated in the Rag2(-/-) gammaC(-/-) C5(-/-) mouse in which muscle degeneration is induced by cryoinjury. We demonstrate that human muscle-derived CD133+ cells showed a much greater regenerative capacity when compared to human myoblasts. The number of fibers expressing human proteins and the number of human cells in a satellite cell position are all dramatically increased when compared to those observed after injection of human myoblasts. In addition, CD133+/CD34+ cells exhibited a better dispersion in the host muscle when compared to human myoblasts. We propose that muscle-derived CD133+ cells could be an attractive candidate for cellular therapy.

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Section: Discussionmentioning

confidence: 99%

In Vivo Myogenic Potential of Human CD133+ Muscle-derived Stem Cells: A Quantitative Study

et al. 2009

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“…In vitro expansion of donor mouse (Montarras et al 2005) and chicken satellite cells (O'Neill and Stockdale 1972) for only a short time significantly reduces the number of muscle fibers they form in vivo, probably because they commence myogenic differentiation. Similar to mouse, the regenerative capacity of human mpc is reduced after they have been expanded in vitro (Cooper et al 2003;Brimah et al 2004), which may be as a result of senescence during the culture period (Decary et al 1996). This suggests that expansion of both mouse and human mpc in vitro may cause stem-like properties to be outweighed and therefore lost.…”

Section: Satellite Cell Contribution To Skeletal Muscle Regenerationmentioning

confidence: 99%

Are Human and Mouse Satellite Cells Really the Same?

Boldrin¹,

Muntoni²,

Morgan³

2010

J Histochem Cytochem.

117

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S U M M A R Y Satellite cells are quiescent cells located under the basal lamina of skeletal muscle fibers that contribute to muscle growth, maintenance, repair, and regeneration. Mouse satellite cells have been shown to be muscle stem cells that are able to regenerate muscle fibers and self-renew. As human skeletal muscle is also able to regenerate following injury, we assume that the human satellite cell is, like its murine equivalent, a muscle stem cell. In this review, we compare human and mouse satellite cells and highlight their similarities and differences. We discuss gaps in our knowledge of human satellite cells, compared with that of mouse satellite cells, and suggest ways in which we may advance studies on human satellite cells, particularly by finding new markers and attempting to re-create the human satellite cell niche in vitro. (J Histochem Cytochem 58:941-955, 2010)

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“…In muscular dystrophies, one concern about therapies that may increase muscle cell proliferation is that the replicative potential of satellite cells will become exhausted. Telomere shortening occurs on chromosomes as cells undergo numerous proliferative events, notable in DMD cells in culture (Cooper et al, 2003;Renault et al, 2002;Thornell et al, 2003), and it is well-established by modeling in cultures of muscle cells (derived from satellite cells) and single muscle fibres, that a gradual loss of proliferative capacity occurs with age and muscular dystrophy (Bockhold et al, 1998;Cooper et al, 2003;Jejurikar and Kuzon, Jr, 2003;Lagord et al, 1998;Renault et al, 2002). This 'bankruptcy' was demonstrated in vivo by experiments using repetitive muscle damage protocols that approached 50 events, exhausting the ability of satellite cells to proliferate and regenerate new muscle (Luz et al, 2002).…”

Section: Satellite Cells In Muscle Plasticitymentioning

confidence: 99%

The satellite cell as a companion in skeletal muscle plasticity:currency, conveyance, clue, connector and colander

Anderson

2006

Journal of Experimental Biology

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THE JOURNAL OF EXPERIMENTAL BIOLOGY 2277 Satellite cells in muscle plasticity formed cells fusing to form myoblasts and finally multinucleated muscle fibres.'That conference was a landmark in studies of the satellite cell and muscle regeneration, and was attended by many of the notable and pioneering contributors to the field of muscle development and muscle regeneration. Muir summarized the debate on a working definition of the satellite cell, as follows (Muir, 1970):'The satellite cell of striated muscle can be defined as a mononucleated cell, whose cytoplasm does not contain myofilaments, and which is enclosed by or lies within the basement membrane component of the sarcolemma of the striated muscle fibre. This definition does not exclude cells which are partially or completely separated from the muscle fibre plasma membrane by extensions of the basement membrane, providing that the basement membrane forms a complete investment of their outer surfaces. ' Although there were presentations detailing the observed uptake of 3 H-thymidine into nuclei of satellite cells (Hay, 1970), the role of satellite cells as precursors was not fully established in 1969. In fact there was still very strong debate that a muscle fibre could fragment into blastema cells that led to new formation of muscle. Even at this time, there was more than one paper on the appearance of osteogenic and chondrogenic tissues from minces of muscle replaced under the skin, and a report of muscle fibre nuclei contributed by a labelled connective tissue grafted into a salamander limb during regeneration (Steen, 1970).It wasn't until two seminal reports from the laboratory of Dr Charles Leblond at McGill University (Moss and Leblond, 1970;Moss and Leblond, 1971) that skeletal muscle satellite cells were convincingly identified as the source of precursor cells that proliferate and fuse to form new skeletal muscle fibres. Time-course studies of labelled nuclei in growing muscle showed that satellite cells were the only muscle cells that had incorporated 3 H-thymidine. The report followed work on colchicine-treated rats (MacConnachie et al., 1964) that showed mitotic figures only in the peripheral 'satellite' position on muscle fibres. The idea that muscle fibre nuclei give rise to the increasing number of myonuclei during the growth was convincingly ruled out (Enesco and Puddy, 1964). The notion that satellite cells contribute these domains to a growing or regenerating fibre, one at a time, is daunting in light of the expenditure of proliferative energy and fusion events. However, it speaks strongly to one of the major roles of satellite cells as a currency of muscle (see below). Satellite cells were also observed on intrafusal fibres (Katz, 1961). Two types of satellite cells were identified on these socalled muscle spindle fibres and were distinguished from those on extrafusal fibres (Maynard and Cooper, 1973), although at least one would now be called a myoblast.Although typically quiescent in normal adult muscle, satellite cells are generally con...

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Extended AmplificationIn Vitroand Replicative Senescence: Key Factors Implicated in the Success of Human Myoblast Transplantation

Cited by 41 publications

References 51 publications

In Vivo Myogenic Potential of Human CD133+ Muscle-derived Stem Cells: A Quantitative Study

In Vivo Myogenic Potential of Human CD133+ Muscle-derived Stem Cells: A Quantitative Study

Are Human and Mouse Satellite Cells Really the Same?

The satellite cell as a companion in skeletal muscle plasticity:currency, conveyance, clue, connector and colander

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