Extended Characterization of Corynebacterium pyruviciproducens Based on Clinical Strains from Canada and Switzerland

Goldenberger, Daniel; Hinić, Vladimira; Turan, Selda; Schultheiss, Elisabeth; Pacheco, Ana Luisa; Frei, Reno; Bernard, Kathryn

doi:10.1128/jcm.00792-14

Cited by 6 publications

(3 citation statements)

References 5 publications

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“…The principally well-known pyruvate bioproduction microorganisms are fungus like Torulopsis glabrata [ 92 , 93 ] and Saccharomyces cerevisiae [ 94 ], with less research on bacterium. For example, Corynebacterium pyruviciproducens was isolated as candidate strains to produce pyruvate [ 95 , 96 ] and Escherichia coli aceF mutant strains were genetically engineered to ferment pyruvate [ 97 , 98 ]. In our study, Corynebacterium was the only genus predicted association with the formation of pyruvic acid.…”

Section: Resultsmentioning

confidence: 99%

The changes of microbial diversity and flavor compounds during the fermentation of millet Huangjiu, a traditional Chinese beverage

et al. 2022

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Huangjiu is a national alcoholic beverage in China. Millet has congenital advantages in development and utilization of nutrient. Brewing Huangjiu with millet can increase the value of millet. Microbial community plays crucial roles in millet Huangjiu fermentation. Flavor compounds reflect the quality and health function of Huangjiu. The flavor compounds of Huangjiu are complex and their formation is closely associated with microorganisms, but the relationship between them during fermentation has been unknown. In this research, this relationship during millet Huangjiu fermentation were deeply investigated. Totally 86 volatile compounds were detected. Bacillus, Weissella, Paenibacillus, Klebsiella, Prevotella was investigated as the dominant microbes through high-throughput sequencing. 537 correlations between major flavor compounds and microbes were established to reflect the dynamic change during millet Huangjiu fermentation. The top five dominant genus of flavor producing microbes were Chryseobacterium, Sporolactobacillus, Psychrobacter, Sphingobium and Anoxybacillus. The content of malic acid and citric acid was gradually improved all through the millet Huangjiu fermentation. Malic acid and citric acid generated from millet Huangjiu fermentation shows healthy properties as liver protection and eliminating fatigue. Our research provides essential information on microbial community succession and the flavor formation during millet Huangjiu fermentation, and beneficial for development of Huangjiu products.

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Section: Resultsmentioning

confidence: 99%

The changes of microbial diversity and flavor compounds during the fermentation of millet Huangjiu, a traditional Chinese beverage

et al. 2022

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“…Subsequent identification was performed using MALDI-TOF MS (Microflex, Bruker Daltonics, Bremen, Germany) using the database DB-5989 (May 2015; [22]). Biochemical identification was used to confirm the MALDI-TOF MS results (API Coryne, bioM erieux, Marcy-l' Etoile, France [25,26]). Resistance testing was performed with Etests (bioM erieux) and MICs of penicillin, ciprofloxacin and vancomycin were interpreted according to EUCAST recommended breakpoints (v6, valid 1 January 2016) and MICs of ceftriaxone and imipenem according to CLSI recommended breakpoints (M45, 3 October 2015).…”

Section: Microbiological Identification and Resistance Testingmentioning

confidence: 99%

Outbreak investigation for toxigenic Corynebacterium diphtheriae wound infections in refugees from Northeast Africa and Syria in Switzerland and Germany by whole genome sequencing

Meinel

Kuehl

Zbinden

et al. 2016

Clinical Microbiology and Infection

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Toxigenic Corynebacterium diphtheriae is an important and potentially fatal threat to patients and public health. During the current dramatic influx of refugees into Europe, our objective was to use whole genome sequencing for the characterization of a suspected outbreak of C. diphtheriae wound infections among refugees. After conventional culture, we identified C. diphtheriae using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) and investigated toxigenicity by PCR. Whole genome sequencing was performed on a MiSeq Illumina with >70×coverage, 2×250 bp read length, and mapping against a reference genome. Twenty cases of cutaneous C. diphtheriae in refugees from East African countries and Syria identified between April and August 2015 were included. Patients presented with wound infections shortly after arrival in Switzerland and Germany. Toxin production was detected in 9/20 (45%) isolates. Whole genome sequencing-based typing revealed relatedness between isolates using neighbour-joining algorithms. We detected three separate clusters among epidemiologically related refugees. Although the isolates within a cluster showed strong relatedness, isolates differed by >50 nucleotide polymorphisms. Toxigenic C. diphtheriae associated wound infections are currently observed more frequently in Europe, due to refugees travelling under poor hygienic conditions. Close genetic relatedness of C. diphtheriae isolates from 20 refugees with wound infections indicates likely transmission between patients. However, the diversity within each cluster and phylogenetic time-tree analysis suggest that transmissions happened several months ago, most likely outside Europe. Whole genome sequencing offers the potential to describe outbreaks at very high resolution and is a helpful tool in infection tracking and identification of transmission routes.

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“…However, its susceptibilities to beta-lactams, erythromycin, clindamycin, fluoroquinolones, tetracycline, and trimethoprim-sulfamethoxazole are variable. Details such as pathogenicity have not yet been elucidated, but some were detected in urine culture [ 14 ]. In the present case, only 10 2 CFU/mL of C. pyruviciproducens was detected in the urine culture, which implies the possibility of contamination with C. pyruviciproducens.…”

Section: Discussionmentioning

confidence: 99%

Acute Bacterial Prostatitis in a 12-Year-Old Boy Without Any Underlying Disease

Kinoshita,

Suzuki,

Koketsu

et al. 2024

Cureus

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Acute bacterial prostatitis (ABP) is a common disease in adults but uncommon in children. Here, we report the case of a pediatric patient without any underlying disease who was diagnosed with ABP while trying to determine the cause of fever refractory to antimicrobial therapy. A previously healthy 12-year-old boy presented with a 13-day history of fever and malaise despite initial antimicrobial treatment. Further tests revealed pyuria and enlarged prostate with possible abscesses, which led to the diagnosis of ABP based on a contrast-enhanced computed tomography (CT) scan. Although initial urine cultures were negative, Corynebacterium pyruviciproducens was detected in subsequent cultures. Antimicrobial therapy for 10 weeks led to improvement without relapse. This case demonstrates that ABP can cause fever in children. Moreover, it shows that contrast-enhanced CT imaging can help identify the cause of fever and that administration of antimicrobials before adequate investigations can confound the diagnosis and complicate the treatment.

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Extended Characterization of Corynebacterium pyruviciproducens Based on Clinical Strains from Canada and Switzerland

Cited by 6 publications

References 5 publications

The changes of microbial diversity and flavor compounds during the fermentation of millet Huangjiu, a traditional Chinese beverage

The changes of microbial diversity and flavor compounds during the fermentation of millet Huangjiu, a traditional Chinese beverage

Outbreak investigation for toxigenic Corynebacterium diphtheriae wound infections in refugees from Northeast Africa and Syria in Switzerland and Germany by whole genome sequencing

Acute Bacterial Prostatitis in a 12-Year-Old Boy Without Any Underlying Disease

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