Extended depth of field and aberration control for inexpensive digital microscope systems

Tucker, S. C.; Cathey, W. Thomas; Dowski, Edward R.

doi:10.1364/oe.4.000467

Cited by 109 publications

(45 citation statements)

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“…This paraxial model for the cubic phase mask has been thoroughly verified experimentally for low NA systems [3,24].…”

Section: Paraxial Modelmentioning

confidence: 79%

“…The CPM has since been investigated for use in standard (low aperture) microscopy [24]. The mask can give a ten times increase in the depth of field without loss of transverse resolution.…”

Section: Methods For Extending the Depth Of Fieldmentioning

confidence: 99%

“…We have already obtained the projected pupil function P + (m, n) in (17). Taking the 2D Fourier transform and applying (24) gives the PSF in the focal plane…”

Section: High Aperture Otf Modelmentioning

confidence: 99%

See 2 more Smart Citations

Wavefront Coding Fluorescence Microscopy Using High Aperture Lenses

Arnison¹,

Cogswell²,

Sheppard³

et al. 2003

Springer Series in Optical Sciences

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“…This paraxial model for the cubic phase mask has been thoroughly verified experimentally for low NA systems [3,24].…”

Section: Paraxial Modelmentioning

confidence: 79%

“…The CPM has since been investigated for use in standard (low aperture) microscopy [24]. The mask can give a ten times increase in the depth of field without loss of transverse resolution.…”

Section: Methods For Extending the Depth Of Fieldmentioning

confidence: 99%

See 1 more Smart Citation

Wavefront Coding Fluorescence Microscopy Using High Aperture Lenses

Arnison¹,

Cogswell²,

Sheppard³

et al. 2003

Springer Series in Optical Sciences

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“…MPoVM is a relatively simple and cost-effective approach, not depending on expensive and vulnerable moving parts, that can be readily implemented on existing fluorescence microscopes. MPoVM can be extended with additional functionality, for example to optically extend the depth of field to up to tens of micrometers [29][30][31][32], or combined with light-sheet excitation and detection [33]. This latter approach, however, will substantially increase the complexity of the current setup.…”

Section: Discussionmentioning

confidence: 99%

High-resolution real-time dual-view imaging with multiple point of view microscopy

Mangeol¹

2016

Biomed. Opt. Express

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Most methods to observe three-dimensional processes in living samples are based on imaging a single plane that is sequentially scanned through the sample. Sequential scanning is inherently slow, which can make it difficult to capture objects moving quickly in three dimensions. Here we present a novel method, multiple point-of-view microscopy (MPoVM), that allows simultaneous capturing of the front and side views of a sample with high resolution. MPoVM can be implemented in most fluorescence microscopes, offering new opportunities in the study of dynamic biological processes in three dimensions. 2. R. Tomer, K. Khairy, F. Amat, and P. J. Keller, "Quantitative high-speed imaging of entire developing embryos with simultaneous multiview light-sheet microscopy," Nat. Methods 9(7), 755-763 (2012). 3. U. Krzic, S. Gunther, T. E. Saunders, S. J. Streichan, and L. Hufnagel, "Multiview light-sheet microscope for rapid in toto imaging," Nat. Methods 9(7), 730-733 (2012) imaging using single-objective SPIM," Nat. Methods 12(7), 641-644 (2015). 34. S.-I. Chang and J.-B. Yoon, "Shape-controlled, high fill-factor microlens arrays fabricated by a 3D diffuser lithography and plastic replication method," Opt. Express 12(25), 6366-6371 (2004).

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“…Because of relative simplicity in manufacture, the cubic phase mask is the most widely used form in the WFC technique [6][7][8]. Various optimized methods have been used to improve the performance of the cubic phase mask (CPM) [9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Optimization of Wavefront Coding Phase Mask Applied to 5X-40X Micro-Objectives Simultaneously

Liu

Miao

Sui

et al. 2015

Journal of the Optical Society of Korea

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A wavefront coding (WFC) technique provides an extension of the depth of field for a microscopy imaging system with slight loss of image spatial resolution. Through the analysis of the relationship between the incidence angle of light at the phase mask and the system pupil function, a mixing symmetrical cubic phase mask (CPM) applied to 5X-40X micro-objectives is optimized simultaneously based on point-spread function (PSF) invariance and nonzero mean values of the modulation transfer function (MTF) near the spatial cut-off frequency. Optimization results of the CPM show that the depth of field of these micro-objectives is extended 3-10 times respectively while keeping their resolution. Further imaging simulations also prove its ability in enhancing the defocus imaging.

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Extended depth of field and aberration control for inexpensive digital microscope systems

Cited by 109 publications

References 0 publications

Wavefront Coding Fluorescence Microscopy Using High Aperture Lenses

Wavefront Coding Fluorescence Microscopy Using High Aperture Lenses

High-resolution real-time dual-view imaging with multiple point of view microscopy

Optimization of Wavefront Coding Phase Mask Applied to 5X-40X Micro-Objectives Simultaneously

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