Extended solubility parameters and stationary phase selectivities of anino-, cyano- and diol-silica normal bonded-phase liquid chromatography columns

The isolation of water-soluble natural products – challenges, strategies and perspectives

“…It has been recently stated that the use of such stationary phases has been largely underestimated for liquid chromatography separations, 244 even though reports on properties and versatile applications of amino-, cyano- and diol-bonded Si-gel stationary phases were available for decades. 245–249…”

“…It has been recently stated that the use of such stationary phases has been largely underestimated for liquid chromatography separations, 244 even though reports on properties and versatile applications of amino-, cyano-and diolbonded Si-gel stationary phases were available for decades. [245][246][247][248][249] In our 1996-2009 survey of 4859 articles reporting the isolation of natural products, 34 articles (0.7%) reported the use of an aminopropyl-derivatized Si-gel column in at least one of the separation steps. Among the 34 articles that included the use of an aminopropyl-derivatized Si-gel column, 17 articles (50%) reported the isolation of alkaloids, 6 articles reported glycosides (18%), 3 articles (9%) reported peptides, and four articles (12%) reported polyphenols and polyols.…”

“…The isolation of the rst mycosporine (P-310, 244) 765 from the fungus Stereum hirsutum was accomplished by IEC, 775 as well as the isolation of mycosporine-Gly (245) from the cnidarian Palythoa tuberculosa. 776 Further investigations of Palythoa tuberculosa extracts led to the isolation of palythene (246), palythine (247) and palythinol (248) using separations on TSK G3000S (polystyrene gel), IEC on Dowex 50W and nal purication by preparative TLC. 777 Porphyra-334 (249) was isolated from the red algae Porphyra tenera by gel ltration on Sephadex G-10, IEC on DEAE Sephadex A-25, carbon column chromatography on Norit-A and IEC on CM Sephadex C-25.…”