Extended Spectrum Beta Lactamase (ESBL) Producing Klebsiella pneumoniae , a therapeutic challenge.

Chaudhary, Raina; Bhatt, Sabita Bhatt; Piya, E

doi:10.3126/mjsbh.v13i1.12996

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Frequency of ESBL production is comparatively higher in Klebsiella, for instance a study conducted in Nigeria demonstrated that 38% K. pneumoniae were ESBL producer as compared to 31% E. coli (Akanbi et al, 2013). In Nepal,[21][22][23][24][25][26][27][28][29][30][31][32][33][34][35][36][37][38].5% of K. pneumoniae were found ESBL producer (Chaudhary et al, 2015;Nepal et al, 2017). The high magnitude of ESBLs may need attributed to lack of antibiotic surveillance, antibiotic misuse and weak infection control measures (Teklu et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Antibacterial Activity of Lemon Juice on Multi-Drug Resistant Klebsiella species Harboring blaOXA-48 Gene

Malla

Khanal

Sudeep

et al. 2022

J. Inst. Sci. Tech.

View full text Add to dashboard Cite

Klebsiella species cause a wide range of infections, and infections caused by multi-drug resistant species have significantly higher rates of morbidity and mortality. Extended spectrum β-lactamase ESBL prodan emergingd carbapenem resistant K. pneumoniae is emerging problem in hospital settings worldwise. However, the distribution of blaOXA-48 in Klebsiella pneumoniae has rarely been described from Nepal. This study was carried out to evaluate in vitro antibacterial activity of lemon juice against clinical isolates of Klebsiella having blaOXA-48 gene. A total of 667 clinical specimens were processed for the identification of Klebsiella species by morphological, cultural and biochemical tests. Methods of antimicrobial susceptibility testing and confirmation of extended spectrum beta lactamase (ESBL) were adopted from the guidelines of the Clinical Laboratory Standard Institute (2018), and blaOXA-48 gene was detected by polymerase chain reaction. The antibacterial activity of lemon juice was assayed using agar well diffusion, and the bactericidal effect of lemon juice was determined by the broth dilution method. Out of 204 culture positive isolates, 29.5% were Klebsiella species of which 16.7% were ESBL producers. Among 19 isolates positive to Modified Hodge test, 68.4% were detected with the plasmid mediated blaOXA-48 gene. The MIC of lemon juice ranged from 12.5-50% (v/v), and the bacteriostatic activity was observed after 6-8 hours of exposure. The tested varieties of lemon juice showed potential inhibitory activity against Klebsiella species indicating that lemon juice has the potential to be used as an antibacterial agent.

show abstract