Extended-Spectrum β-Lactamase and AmpC β -Lactamase-Producing Bacteria in Livestock Animals

Ewers, Christa

doi:10.1007/978-94-017-9457-2_15

Cited by 3 publications

(2 citation statements)

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“…Despite the reported evidence on the increasing detection of AmpC beta-lactamases among Enterobacteriaceae worldwide [22,32,33], little or no data has been generated for South African strains and this highlights the importance of the present study. In the current study area, strains belonging to the family Enterobacteriaceae have previously been isolated from various sources and their antimicrobial susceptibility profiles determined [34][35][36][37][38]. Moreover, previous studies have indicated that besides humans and livestock, food products can also harbour AmpC betalactamases-producing Gram-negative bacteria [2,22,30,[39][40][41][42].…”

Section: Introductionmentioning

confidence: 99%

Evidence of potentially unrelated AmpC beta-lactamase producing Enterobacteriaceae from cattle, cattle products and hospital environments commonly harboring the blaACC resistance determinant

et al. 2021

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The occurrence and genetic relatedness of AmpC beta-lactamase producing Enterobacteriaceae isolated from clinical environments, groundwater, beef, human and cattle faeces were investigated. One hundred seventy-seven (177) samples were collected and cultured on MacConkey agar. A total of 203 non-repetitive isolates were characterised using genus/species-specific PCRs and the identified isolates were subjected to antibiotic susceptibility testing. The production of AmpC beta-lactamases was evaluated using cefoxitin disc, confirmed by the D96C detection test and their encoding genes detected by PCR. The D64C extended-spectrum beta-lactamases (ESBL) test was also performed to appraise ESBLs/AmpC co-production. The genetic fingerprints of AmpC beta-lactamase producers were determined by ERIC-PCR. A total of 116 isolates were identified as E. coli (n = 65), Shigella spp. (n = 36) and Klebsiella pneumoniae (n = 15). Ciprofloxacin resistance (44.4–55.4%) was the most frequent and resistance against the Cephem antibiotics ranged from 15–43.1% for E. coli, 25–36.1% for Shigella spp., and 20–40% for K. pneumoniae. On the other hand, these bacteria strains were most sensitive to Amikacin (0%), Meropenem (2.8%) and Piperacillin-Tazobactam (6.7%) respectively. Nineteen (16.4%) isolates comprising 16 E. coli and 3 Shigella spp. were confirmed as AmpC beta-lactamase producers. However, only E. coli isolates possessed the corresponding resistance determinants: blaACC (73.7%, n = 14), blaCIT (26%, n = 5), blaDHA (11%, n = 2) and blaFOX (16%, n = 3). Thirty-four (27.3%) Enterobacteriaceae strains were confirmed as ESBL producers and a large proportion (79.4%, n = 27) harboured the blaTEM gene, however, only two were ESBLs/AmpC co-producers. Genetic fingerprinting of the AmpC beta-lactamase-producing E. coli isolates revealed low similarity between isolates. In conclusion, the findings indicate the presence of AmpC beta-lactamase-producing Enterobacteriaceae from cattle, beef products and hospital environments that commonly harbour the associated resistance determinants especially the blaACC gene, nonetheless, there is limited possible cross-contamination between these environments.

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Section: Introductionmentioning

confidence: 99%

Evidence of potentially unrelated AmpC beta-lactamase producing Enterobacteriaceae from cattle, cattle products and hospital environments commonly harboring the blaACC resistance determinant

et al. 2021

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“…In the EU, CTX-M-1 is the most frequently reported ESBL subtype in E. coli originating from food producing animals (poultry, cattle and pigs) and food [8]. By contrast, in Asia, CTX-M-2, CTX-M-14 and CTX-M-15 are found in cattle and pigs [9].…”

Section: Introductionmentioning

confidence: 99%

First Detection of Extended-Spectrum ß-Lactamase Producing Escherichia coli in Breeder Pigs in Bhutan

2017

ARC Journal of Animal and Veterinary Sciences

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Epidemiology of Extended-Spectrum β-Lactamase-Producing Escherichia coli in the Human-Livestock Environment

Nüesch‐Inderbinen

Stephan

2016

Curr Clin Micro Rpt

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The detection of extended-spectrum -lactamase (ESBL) producers in the fecal flora of healthy food-producing animals has increased in recent years. This is mainly attributed to the intense use of antibiotics in this sector. There is growing concern regarding the risk of spread of such bacteria, especially Escherichia coli and Salmonella, to humans and to the environment. The occurrence of ESBL producers in the major groups of livestock, i.e., poultry, pigs, cattle, and sheep is highlighted and discussed with regard to data that provide evidence for transmission of their resistance traits from livestock to humans and to farm environments. AbstractThe detection of extended-spectrum ß-lactamase (ESBL) producers in the fecal flora of healthy food-producing animals has increased in recent years. This is mainly attributed to the intense use of antibiotics in this sector. There is growing concern regarding the risk of spread of such bacteria, especially Escherichia coli and Salmonella, to humans and to the environment. The occurrence of ESBL producers in the major groups of livestock, i.e., poultry, pigs, cattle and sheep is highlighted and discussed with regard to data that provide evidence for transmission of their resistance traits from livestock to humans and to farm environments.

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Extended-Spectrum β-Lactamase and AmpC β -Lactamase-Producing Bacteria in Livestock Animals

Cited by 3 publications

References 135 publications

Evidence of potentially unrelated AmpC beta-lactamase producing Enterobacteriaceae from cattle, cattle products and hospital environments commonly harboring the blaACC resistance determinant

Evidence of potentially unrelated AmpC beta-lactamase producing Enterobacteriaceae from cattle, cattle products and hospital environments commonly harboring the blaACC resistance determinant

First Detection of Extended-Spectrum ß-Lactamase Producing Escherichia coli in Breeder Pigs in Bhutan

Epidemiology of Extended-Spectrum β-Lactamase-Producing Escherichia coli in the Human-Livestock Environment

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