Extended-Spectrum β-Lactamase CTX-M-15-Producing Klebsiella pneumoniae of Sequence Type ST274 in Companion Animals

Abstract

Screening of extended-spectrum ␤-lactamase (ESBL)-producing Gram-negative bacteria in companion animals living in the Paris area in France identified a high rate of CTX-M-15-producing Klebsiella pneumoniae. Those isolates were recovered during the 2010-2011 period from both infections and asymptomatic colonizations. Sequence typing revealed that most of these isolates belonged to sequence type ST274. Interestingly, the bla CTX-M-15 gene was located on a specific and novel plasmid scaffold. These findings highlight that companion animals may be reservoirs for CTX-M-15-producing K. pneumoniae evolving separately from the human reservoir of CTX-M-15 producers.

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Dogs

“…The highest E. coli isolation prevalence (40.7%) recorded in this study differs from the result of a similar study conducted in Enugu State, Nigeria, in which Klebsiella was the dominant (67.4% isolation prevalence) enterobacteria genus isolated from healthy pigs . The result of this study, also contrasted Poirel et al (2013) who reported Klebsiella, with 51.5% prevalence, as the dominant genus among 33 ESBLproducing enterobacterial isolates from dogs/cats/others in France.…”

“…An Iranian study also reported lower ESBL-producing E. coli isolation prevalence in faecal samples of healthy cats (Akhtardanesh et al, 2015). The E. coli isolation prevalence in the present study, is however, lower than ESBL-producing E. coli isolation prevalence recorded among 20 faecal ESBL-producing enterobacterial isolates from healthy dogs/cats in Romania (Cozma et al, 2015b), 63 faecal E. coli isolates from hospitalized dogs in South Korea (So et al, 2012), 33 ESBL-producing enterobacterial isolates from companion animals in France (Poirel et al, 2013), 15 E. coli isolates from urine samples of 138 sick cats in Italy (Nebia et al, 2014) and in faecal samples of 20 each of healthy and diarrhoeic dogs in the Netherlands (Hordjik et al, 2013), respectively. It is also lower than 62% ESBL-resistant E. coli isolation prevalence in faecal samples from 13 sick dogs reported by Damborg et al (2011) in Denmark.…”

“…It is also higher than 9, 28.9, 2 and 3.6% ESBLresistant/producing enterobacterial isolation prevalence in 22 household dogs in Sweden, 58 faecal samples of healthy dogs, among clinical enterobacterial isolates from 65 dogs/cats/horses and 608 dogs/cats reported by Ljungquist et al (2016), Cozma et al (2015a), Dierikx et al (2012) and Bogaerts et al (2015) in Sweden, Romania, The Netherlands and Europe, respectively. The result is however lower than 62.1 and 30% ESBL-producing enterobacterial isolation prevalence in faecal samples of 29 healthy dogs/cats and among 110 dogs/cats/others reported by Cozma et al (2015b) and Poirel et al (2013) in Romania and France, respectively. A longitudinal study conducted in the Netherlands, reported 45-63% ESBL-producing enterobacterial isolation prevalence in 25 faecal samples of 38 dogs (Baede et al, 2015); this result is also higher than the ESBL-resistant enterobacteria prevalence (27%) recorded in the present study.…”

“…It is lower than 64.3, 66.7, 33.3, 100 and 20% gentamicin resistance observed among ESBL-resistant / producing E. coli isolates from extra-clinical faecal samples from dogs in Germany (Schaufler et al, 2015), stray dogs in South Korea (Tamang et al, 2012), sick/healthy dogs/cats/rat in Italy (Caratolli et al, 2005), and sick cats/dogs in Switzerland (Huber et al, 2013), respectively. The 10% gentamicin resistance among Klebsiella isolates in this study is lower when compared with 100% gentamicin resistance among 16 ESBLproducing Klesbsiella isolates from rectal/urine samples of companion/domestic animals reported by Poirel et al (2013) in France. The 27.2% E. coli streptomycin resistance in this study is lower than 50, 66.7 and 75% streptomycin resistance reported by Tamang et al (2012), Caratolli et al (2005) and Costa et al (2004) in South Korea, Italy and Portugal, respectively.…”

“…Surveillance studies to assess dogs as potential reservoirs and disseminators of ESBL-resistant bacteria have been conducted in several countries in Europe (Costa et al, 2004;Caratolli et al, 2005;Poirel et al, 2013;Gandolfi-Decristophoris et al, 2013;Hordijk et al, 2013;Johard et al, 2015;Cozma et al, 23 …”