Extended Spectrum β Lactamase producing Klebsiella pneumoniae in neonatal intensive care unit

Krishna, Bhuvana; Patil, Ajinkya; Chandrasekhar, M. R.

doi:10.1007/s12098-007-0111-1

Cited by 22 publications

(13 citation statements)

References 9 publications

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“…They developed quick screening methods to assess the different mechanisms of ESBL production, so that the patients can be treated with appropriate antibiotics. Prevalence of ESBLs is reported to be high from Medicine ICU, Surgery wards and NICU/ Pediatric wards [17,[27][28][29][30]. Our study correlates with these studies.…”

Section: Discussionsupporting

confidence: 89%

Prevalence and Characterization of Extended Spectrum Beta-Lactamase Production in Clinical Isolates of Klebsiella pneumonia

Begum¹,

Damle²

2015

J Med Microb Diagn

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Aim: This study was carried out to determine the presence of TEM and SHV genes in extended-spectrum β-lactamase (ESBL) producing Klebsiella pneumoniae. The study was also aimed to compare results of phenotypic confirmatory double disc diffusion test and genotypic methods. Materials and Methods:A total of 679 strains of Klebsiella pneumoniae were selected for the study from June 2012-December 2013. Kirby -Bauer disk diffusion method was performed to determine the antibiotic resistance pattern. Screened for ESBL and confirmed by phenotypic confirmatory disc diffusion test (PCDDT). 100 randomly selected isolates were investigated for the presence of TEM and SHV genes via Polymerase chain reaction (PCR) using two different sets of primers. Multiplex PCR was also performed for the same.Results: Phenotypic confirmatory test was able to detect ESBL production in 90.13% of Klebsiella pneumoniae isolates. Among the two ESBL genotypes, the most prevalent genotype was found to be TEM. Majority of ESBL producing isolates possess both ESBL genes. Conclusion:Multiplex PCR can be used as a rapid method to identify common genes (TEM and SHV) responsible for extended spectrum beta lactamase production in Klebsiella pneumoniae. It will prove valuable for surveillance and for determining the line of treatment against drug resistant organisms, thus saving precious time and resources. PCDDT results correlated with genotypic method in all the tested strains.

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Section: Discussionsupporting

confidence: 89%

Prevalence and Characterization of Extended Spectrum Beta-Lactamase Production in Clinical Isolates of Klebsiella pneumonia

Begum¹,

Damle²

2015

J Med Microb Diagn

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“…The patients carrying these lineages also lacked overlapping ICU admissions (Supplementary Figure 2). Such lineage sharing could result from independent acquisition in the community prior to hospital admission, but could also potentially be explained by cryptic longer-term transmission chains involving bacteria that persist within the hospital between admissions (for example, in reservoirs such as sinks or drains, or colonisation of healthcare workers [22][23][24] ). In the latter case, we would expect the genetic distances between isolates in these clusters to be consistent with the accumulation of genetic variation over time in an evolving reservoir or transmission chain, which could exceed the 25-SNP cut-off if sufficient time has passed between isolations.…”

Section: Klebsiella Transmission In the Icumentioning

confidence: 99%

Gastrointestinal carriage is a major reservoir ofK. pneumoniaeinfection in intensive care patients

Gorrie

Mirčeta

Wick

et al. 2016

Preprint

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BackgroundKlebsiella pneumoniae is an opportunistic pathogen and a leading cause of hospitalassociated (HA) infections. Patients in intensive care units (ICUs) are particularly at risk, and outbreaks are frequently reported in ICUs. K. pneumoniae is also part of the healthy human microbiome, providing a potential reservoir for HA infection. However, the frequency of K. pneumoniae gut colonization and its contribution to HA infections are not well characterized.

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“…Sporadic reports document the role of hands in spreading infection and isolated efforts at improving hand hygiene across the country. [47][48][49][50] It is necessary to increase the pace of activities to reduce HCAI and to effectively engage millions of HCWs in different types of health-care facilities in this effort. The Director General of Health Services of India confirmed his resolve to promote activities at a national level to reduce HCAI by signing a commitment with CCiSC on July 14 2006.…”

Section: Implementation Strategiesmentioning

confidence: 99%

Prevention and control of health care-associated infections through improved hand hygiene

Mathai

Allegranzi

Kilpatrick

et al. 2010

Indian Journal of Medical Microbiology

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Determined actions are required to address the burden due to health care-associated infections worldwide and improve patient safety. Improving hand hygiene among health care workers is an essential intervention to achieve these goals. The World Health Organization (WHO) First Global Patient Safety Challenge, Clean Care is Safer Care, pledged to tackle the problem of health care-associated infection at its launch in 2005 and has elaborated a comprehensive set of guidelines for use in both developed and developing countries worldwide. The final version of the WHO Guidelines on Hand Hygiene in Health Care was issued in March 2009 and includes recommendations on indications, techniques, and products for hand hygiene. In this review, we discuss the role of hands in the transmission of health care-associated infection, the benefits of improved compliance with hand hygiene, and the recommendations, implementation strategies and tools recommended by WHO. We also stress the need for action to increase the pace with which these recommendations are implemented in facilities across India.

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Extended Spectrum β Lactamase producing Klebsiella pneumoniae in neonatal intensive care unit

Cited by 22 publications

References 9 publications

Prevalence and Characterization of Extended Spectrum Beta-Lactamase Production in Clinical Isolates of Klebsiella pneumonia

Prevalence and Characterization of Extended Spectrum Beta-Lactamase Production in Clinical Isolates of Klebsiella pneumonia

Gastrointestinal carriage is a major reservoir ofK. pneumoniaeinfection in intensive care patients

Prevention and control of health care-associated infections through improved hand hygiene

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