Extended-Spectrum β-Lactamase-Producing Escherichia coli Isolated from Food-Producing Animals in Tamaulipas, Mexico

Abstract

Extended-spectrum β-lactamase (ESBL)-producing E. coli has become an important global problem for the public health sector. This study aims to investigate the E. coli antimicrobial resistance profile among living food-producing animals in Tamaulipas, Mexico. A total of 200 fecal samples were collected from bovines, pigs, chickens and sheep. A total of 5.0% of the strains were phenotypically confirmed as ESBL producers. A high percentage of phenotypic antimicrobial resistance was observed against gentamicin (93.3%), tetracycline (86.6%) and streptomycin (83.3%). The gentamicin-resistant strains showed MDR, distributed among 27 resistance patterns to different antimicrobials. The antimicrobial resistance gene tet(A) was detected in 73.3% of isolates, aadA1 in 60.0% and sul2 in 43.3% of strains. The blaCTX-M gene was found in 23.3% of strains. The virulence gene hlyA was detected in 43.3% of isolates; stx1 and stx2 were not detected in any strain. The phylotyping indicated that the isolates belonged to groups A (33.3%), B1 (16.6%), B2 (40.0%) and D (10.0%). These results show that food-producing animals might be a reservoir of ESBL-producing bacteria and may play a role in their spread.

Molecular detection of Shiga toxin and extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates from sheep and goats

“…Due to its ubiquitous nature and sharing the same niche as other enteric pathogens and being transmissible by the same route, it contributes to the dissemination of antimicrobial resistance (AMR) [ 7 ]. Globally, AMR is considered a major threat to public health [ 7 , 8 ] and affects both clinical settings and the community at large [ 9 ]. In the sentinel organism E. coli , resistance proportions are often called “prevalence” [ 10 ].…”

Multidrug-resistance and extended-spectrum beta-lactamase-producing lactose-fermenting enterobacteriaceae in the human-dairy interface in northwest Ethiopia

“…However, ESBLs are sensitive to β-lactamase inhibitors like clavulanic acid, sulbactam, and tazobactam and they cannot hydrolyze carbapenems and cephamycins. Bacteria that produce β-lactamases are usually MDR and are linked to life-threatening hospital or community-acquired infections [ 12 , 13 ]. They are associated with a higher proportion of treatment failure and mortality than infections caused by non-ESBL producers [ 14 – 16 ].…”