Extended-Spectrum-β-Lactamase (TEM-52)-Producing Strains of Salmonella enterica of Various Serotypes Isolated in France

Abstract

From 2002 to 2003, four isolates of Salmonella enterica serotypes Typhimurium, Enteritidis, Blockley, and Panama, isolated in France from patients with gastroenteritis, were found to produce extended-spectrum ␤-lactamase TEM-52. The study showed the bla TEM-52 gene to be located in a Tn3-like structure and carried by 100-or 32-kb conjugative plasmids.

Multidrug Resistance in Salmonella enterica Serotype Typhimurium from Humans in France (1993 to 2003)

“…One isolate was positive for both the bla TEM and bla OXA-30 genes, and the ESBL-producing isolate was positive for the bla TEM-52 gene (43). No bla SHV gene was detected.…”

“…Three other TEM-52-producing S. enterica isolates of different serotypes were isolated from human cases (without prior treatment with ESC and without recent hospitalization) in France in 2002 and 2003. A common plasmid carrying bla TEM-52 was found in three of the four isolates (43). As TEM-52 has been identified increasingly in various serotypes of Salmonella in poultry in The Netherlands (14) and in Belgium (A. Cloeckaert, unpublished results) since 2001, we can hypothesize that poultry is the source for human contamination.…”

IncI1 Plasmid Carrying Extended-Spectrum-β-Lactamase Gene bla CTX-M-1 in Salmonella enterica Isolates from Poultry and Humans in France, 2003 to 2008

“…In Belgium and France, the emergence of resistance to ESCs, due to extendedspectrum ␤-lactamases (ESBLs), in E. coli and S. enterica from animal (mainly cattle and poultry) and human origins (1,3,6,7,10,11,14,15,17) has been reported. Resistance in these bacteria was reported to be conferred mainly by the ESBL genes bla CTX-M-2 , bla CTX-M-9 , bla CTX-M- 15 , and bla (1,3,6,11,14,15,17). In addition, the occurrence of CTX-M-1 ESBL resistance in E. coli isolates recovered from food animals (cattle, poultry, and swine) in France was recently reported (7,10,11).…”

“…Antibiotic susceptibility testing was done by the disc diffusion method, and MICs of ceftriaxone and ceftazidime were determined by Etest as described previously (1,15,17). Resistance to ESCs was transferred from all Salmonella strains to E. coli recipient strain J5 (resistant to rifampin) by conjugation as previously described (1,15,17).…”

Repeated Occurrence of Diverse Extended-Spectrum β-Lactamases in Minor Serotypes of Food-BorneSalmonella entericasubsp.enterica

“…Emergence of domestically acquired TEM-52-producing NTS human isolates of serotypes Typhimurium, Enteritidis, Blockley, and Panama has also been reported recently in France and the United Kingdom (23,25). Additionally, TEM-52-producing E. coli strains have been isolated recently from healthy and sick animals in Spain and Portugal (2, 4).…”