Extending Hyperspectral Imaging for Plant Phenotyping to the UV-Range

Brugger, Anna; Behmann, Jan; Paulus, Stefan; Luigs, Hans-Georg; Kuśka, Matheus Thomas; Schramowski, Patrick; Kersting, Kristian; Steiner, Ulrike; Mahlein, Anne-Katrin

doi:10.3390/rs11121401

Cited by 36 publications

(20 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The VIS and NIR region (400-1000 nm) have the highest information content for monitoring plant stress. The ultraviolet-range (UV, 250-400 nm) (Brugger et al 2019) and SWIR-range (1000-2500 nm) provide information as well. Spectral sensors can be characterized by resolution (number of wavebands per nm) and the type of the detector.…”

Section: Methods Of Hyperspectral Image Acquisitionmentioning

confidence: 99%

From visual estimates to fully automated sensor-based measurements of plant disease severity: status and challenges for improving accuracy

et al. 2020

Self Cite

View full text Add to dashboard Cite

The severity of plant diseases, traditionally the proportion of the plant tissue exhibiting symptoms, is a key quantitative variable to know for many diseases and is prone to error. Good quality disease severity data should be accurate (close to the true value). Earliest quantification of disease severity was by visual estimates. Sensor-based image analysis including visible spectrum and hyperspectral and multispectral sensors are established technologies that promise to substitute, or complement visual ratings. Indeed, these technologies have measured disease severity accurately under controlled conditions but are yet to demonstrate their full potential for accurate measurement under field conditions. Sensor technology is advancing rapidly, and artificial intelligence may help overcome issues for automating severity measurement under hyper-variable field conditions. The adoption of appropriate scales, training, instruction and aids (standard area diagrams) has contributed to improved accuracy of visual estimates. The apogee of accuracy for visual estimation is likely being approached, and any remaining increases in accuracy are likely to be small. Due to automation and rapidity, sensor-based measurement offers potential advantages compared with visual estimates, but the latter will remain important for years to come. Mobile, automated sensor-based systems will become increasingly common in controlled conditions and, eventually, in the field for measuring plant disease severity for the purpose of research and decision making.

show abstract

Section: Methods Of Hyperspectral Image Acquisitionmentioning

confidence: 99%

From visual estimates to fully automated sensor-based measurements of plant disease severity: status and challenges for improving accuracy

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…To enhance precision of non-invasive estimation of SMs in pepper leaves, future studies should consider hyperspectral sensors as well as chlorophyll fluorescence-based sensors, ideally a combination of both. Sensors covering the UV range are just entering the market and appear as a promising tool to access SMs in plants, as they cover absorption bands of flavones and other phenolic leaf compounds [52].…”

Section: Implications and Future Challengesmentioning

confidence: 99%

Effect of UV Radiation and Salt Stress on the Accumulation of Economically Relevant Secondary Metabolites in Bell Pepper Plants

et al. 2020

View full text Add to dashboard Cite

The green biomass of horticultural plants contains valuable secondary metabolites (SM), which can potentially be extracted and sold. When exposed to stress, plants accumulate higher amounts of these SMs, making the extraction and commercialization even more attractive. We evaluated the potential for accumulating the flavones cynaroside and graveobioside A in leaves of two bell pepper cultivars (Mavras and Stayer) when exposed to salt stress (100 mM NaCl), UVA/B excitation (UVA 4–5 W/m2; UVB 10–14 W/m2 for 3 h per day), or a combination of both stressors. Plant age during the trials was 32–48 days. HPLC analyses proved the enhanced accumulation of both metabolites under stress conditions. Cynaroside accumulation is effectively triggered by high-UV stress, whereas graveobioside A contents increase under salt stress. Highest contents of secondary metabolites were observed in plants exposed to combined stress. Effects of stress on overall plant performance differed significantly between treatments, with least negative impact on above ground biomass found for high-UV stressed plants. The usage of two non-destructive instruments (Dualex and Multiplex) allowed us to gain insights into the ontogenetical effects at the leaf level and temporal development of SM contents. Indices provided by those devices correlate fairly with amounts detected via HPLC (Cynaroside: r2 = 0.46–0.66; Graveobioside A: r2 = 0.51–0.71). The concentrations of both metabolites tend to decrease at leaf level during the ontogenetical development even under stress conditions. High-UV stress should be considered as a tool for enriching plant leaves with valuable SM. Effects on the performance of plants throughout a complete production cycle should be evaluated in future trials. All data is available online.

show abstract

“…These effects should also be taken into account when evaluating the value of production systems that are based on commercialization of both fruits and SMs in leaves of horticultural plants. appear as a promising tool to access SMs in plants, as they cover absorption bands of flavones and other phenolic leaf compounds [46].…”

Section: Implications and Future Challengesmentioning

confidence: 99%

Effect of UV Radiation and Salt Stress on the Accumulation of Economically Relevant Secondary Metabolites in Bell Pepper Plants

Ellenberger¹,

Siefen²,

Krefting³

et al. 2019

Preprint

View full text Add to dashboard Cite

The green biomass of horticultural plants contains valuable secondary metabolites (SM) which can potentially be extracted and sold. When exposed to stress, plants accumulate higher amounts of these SMs, making the extraction and commercialization even more attractive. We evaluated the potential for accumulating of the flavones cynaroside and graveobioside A in leaves of two bell pepper cultivars (Mavras and Stayer) when exposed to salt stress (100 mM NaCl), UVA/B excitation (UVA 4-5 W/m²; UVB 10-14 W/m² for 3 hours per day) or a combination of both stressors. HPLC analyses proved the enhanced accumulation of both metabolites under stress conditions. Cynaroside accumulation is effectively triggered by high-UV stress, whereas graveobioside A contents increase under salt stress. Highest contents were observed in plants exposed to combined stress. Effects of stress on overall plant performance differed significantly between treatments, with least negative impact on aboveground biomass found for high-UV stressed plants. The usage of two non-destructive instruments (Dualex and Multiplex) allowed us to gain insights in ontogenetical effects at the leaf level and temporal development of SM contents over time. Indices provided by those Preprints (www.preprints.org) | NOT PEER-REVIEWED | devices correlate fairly with amounts detected via HPLC (Cynaroside: R 2 = 0.46 -0.66; Graveobioside A: R 2 = 0.51 -0.71). The concentrations of both metabolites tend to decrease at leaf level during the ontogenetical development even under stress conditions. High-UV stress is a promising tool for enriching plant leaves with valuable SM without major effects on plant biomass. All data is available online [1]. Abbreviations DATIdays after treatment inception HPLChigh performance liquid chromatography ROSreactive oxygen species SMsecondary metabolite Introduction Green biomass as a source of valuable chemicalsCommercial vegetable production is accompanied by large quantities of so far under-utilized green biomass in all stages of production and especially after harvest [2]. While the use of biomass for the purpose of energy production is becoming a standard procedure in northern Europe in recent years [3], the extraction and the use of high-value SMs from vegetable plant leaves are just being developed. Research strategies and legacy in Europe are heading towards cascade use of agricultural byproducts and pave the way for extracting and using "valuable substances or molecules before ultimately discarding the left-overs" [4]. The pharmaceutical industryas an exampleis highly dependent on plant SMs, since approximately 60% of anticancer compounds and 75 % of drugs for infectious diseases are derived from plants [5]. In this frame, research on targeted enrichment of valuable substances in plant biomass is gaining in importance [6].Preprints (www.preprints.org) | NOT PEER-REVIEWED |

show abstract

Extending Hyperspectral Imaging for Plant Phenotyping to the UV-Range

Cited by 36 publications

References 30 publications

From visual estimates to fully automated sensor-based measurements of plant disease severity: status and challenges for improving accuracy

From visual estimates to fully automated sensor-based measurements of plant disease severity: status and challenges for improving accuracy

Effect of UV Radiation and Salt Stress on the Accumulation of Economically Relevant Secondary Metabolites in Bell Pepper Plants

Effect of UV Radiation and Salt Stress on the Accumulation of Economically Relevant Secondary Metabolites in Bell Pepper Plants

Contact Info

Product

Resources

About