Extending Single Cell Bioprinting from Femtosecond to Picosecond Laser Pulse Durations

Zhang, Jun; Geiger, Yasemin; Sotier, F.; Djordjevic, Sasa; Docheva, Denitsa; Sudhop, Stefanie; Clausen‐Schaumann, Hauke; Huber, Heinz P.

doi:10.3390/mi12101172

Cited by 9 publications

(5 citation statements)

References 44 publications

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“…LIFT has attracted huge attention as bioprinting technique as well, and in the last 20 years, several works have demonstrated applications of 2D and 3D printing of biomolecules and living cells both for in vitro and in vivo approaches [ 52 - 61 ] , including single cell isolation [ 46 , 47 ] and using different laser pulse duration regimes [ 62 ] . In addition, it has been extensively shown that LIFT is one of the bioprinting techniques with the highest cell viability, even better than the widely applied extrusion or inkjet printing methods.…”

Section: Lasers In Ctcs Researchmentioning

confidence: 99%

“…In our approach, described in detail in the Methods section, we used a donor structure made with a commercial polyimide film with a thickness of 30 μm. The main advantage of using thick polyimide films is its high absorption in the UV range, efficiently absorbing the laser beam and avoiding direct cell irradiation or even heating of the liquid sample [ 62 , 69 , 73 ] . On the other hand, the use of polyimide presents other advantages: the material is partially transparent to the visible light so fluorescence-vision and techniques for cell recognition and selection can be used, even in a compact optical arrangement in which fluorescence and vision arrangements share the same optical path that the laser beam used to produce the liquid transfer [ 73 ] .…”

Section: Lasers In Ctcs Researchmentioning

confidence: 99%

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Laser transfer for circulating tumor cell isolation in liquid biopsy

Molpeceres¹,

Ramos-Medina²,

Márquez³

et al. 2024

IJB

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Cancer research has found in the recent years a formidable ally in liquid biopsy, a noninvasive technique that allows the study of circulating tumor cells (CTCs) and biomolecules involved in the dynamics of cancer spread like cell-free nucleid acids or tumor-derived extracellular vesicles. However, single-cell isolation of CTCs with high viability for further genetic, phenotypic, and morphological characterization remains a challenge. We present a new approach for single CTC isolation in enriched blood samples using a liquid laser transfer (LLT) process, adapted from standard laser direct write techniques. In order to completely preserve the cells from direct laser irradiation, we used an ultraviolet laser to produce a blister-actuated laser-induced forward transfer process (BA-LIFT). Using a plasma-treated polyimide layer for blister generation, we completely shield the sample from the incident laser beam. The optical transparency of the polyimide allows direct cell targeting using a simplified optical setup, in which the laser irradiation module, standard imaging, and fluorescence imaging share a common optical path. Peripheral blood mononuclear cells (PBMCs) were identified by fluorescent markers, while target cancer cells remained unstained. As a proof of concept, we were able to isolate single MDA-MB-231 cancer cells using this negative selection process. Unstained target cells were isolated and culture while their DNA was sent for single-cell sequencing (SCS). Our approach appears to be an effective approach to isolate single CTCs, preserving cell characteristics in terms of cell viability and potential for further SCS.

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Section: Lasers In Ctcs Researchmentioning

confidence: 99%

Section: Lasers In Ctcs Researchmentioning

confidence: 99%

Laser transfer for circulating tumor cell isolation in liquid biopsy

Molpeceres¹,

Ramos-Medina²,

Márquez³

et al. 2024

IJB

View full text Add to dashboard Cite

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“…[ 120 ] The effect of laser pulse duration on cell viability has been investigated, by delivering less energy by extending from femtosecond (600 fs) to picosecond (14.1 ps). [ 197 ] When using optimized conditions, a cell‐survival rate post‐transfer of >95% was achieved across the entire pulse duration range. [ 197 ] A similar effect is seen with light‐based 3D bioprinting; however, UV light can cause harmful effects (mutagenic, carcinogenic) which can damage cells and affect cell viability.…”

Section: Bioprinting For Bone Tissue Engineeringmentioning

confidence: 99%

“…[ 197 ] When using optimized conditions, a cell‐survival rate post‐transfer of >95% was achieved across the entire pulse duration range. [ 197 ] A similar effect is seen with light‐based 3D bioprinting; however, UV light can cause harmful effects (mutagenic, carcinogenic) which can damage cells and affect cell viability. [ 198,199 ] Therefore, there is a drive toward developing visible light‐based 3D bioprinting techniques instead.…”

Section: Bioprinting For Bone Tissue Engineeringmentioning

confidence: 99%

Hydrogels and Bioprinting in Bone Tissue Engineering: Creating Artificial Stem‐Cell Niches for In Vitro Models

et al. 2023

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Advances in bioprinting have enabled the fabrication of complex tissue constructs with high speed and resolution. However, there remains significant structural and biological complexity within tissues that bioprinting is unable to recapitulate. Bone, for example, has a hierarchical organization ranging from the molecular to whole organ level. Current bioprinting techniques and the materials employed have imposed limits on the scale, speed, and resolution that can be achieved, rendering the technique unable to reproduce the structural hierarchies and cell–matrix interactions that are observed in bone. The shift towards biomimetic approaches in bone tissue engineering, where hydrogels provide biophysical and biochemical cues to encapsulated cells, is a promising approach to enhance the biological function and development of tissues for in vitro modelling. A major focus in bioprinting of bone tissue for in vitro modelling is creating the dynamic microenvironmental niches to support, stimulate, and direct the cellular processes for bone formation and remodeling. Hydrogels are ideal materials for imitating the extracellular matrix since they can be engineered to present various cues whilst allowing bioprinting. Here, we review recent advances in hydrogels and 3D bioprinting towards creating a microenvironmental niche that is conducive to tissue engineering of in vitro models of bone.This review focuses on hydrogels and 3D bioprinting in bone tissue engineering for development of in vitro models of bone. It highlights challenges in recapitulating the biological complexity seen in bone and how synergistic application of dynamic hydrogels and innovative bioprinting pipelines could address these challenges to achieve bone models.This article is protected by copyright. All rights reserved

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“…Absorption of the laser pulse in this case occurs only in the microscopic area of laser beam focusing point. Therefore, radiation can be introduced into the hydrogel in different ways: from below through a transparent gel layer (jets and drops fly out upwards) [15][16][17], and from above through the air medium (jets and drops fly out upwards) [18][19][20]. The disadvantages of using ultrashort laser pulses for bioprinting include the high complexity and cost of the femtosecond laser system, the difficulty of precise focusing, and the ultrahigh pressure and temperature jumps that occur during optical breakdown.…”

Section: Introductionmentioning

confidence: 99%

Laser bioprinting without donor plate

Cheptsov¹,

Минаев²,

Zhigarkov³

et al. 2022

Laser Phys. Lett.

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A promising laser bioprinting technology based on the effect of laser-induced direct transfer is rapidly developing and is already actively used in biotechnology and medicine today. Typically, such technologies use donor plates with an absorbent coating, from which gel microdroplets with living cells and microorganisms are transferred to an acceptor substrate under the action of a laser pulse. This article proposes a new bioprinting technology that makes it possible to transfer microdroplet not only without an absorbing coating, but also without the donor plate itself. The essence of this method is that the transfer to the surface of the acceptor is carried out by a series of nanosecond pulse with a wavelength of 3 μm directly from the surface of a liquid with microorganisms. The mechanism of a liquid jet and microdroplets formation upon absorption of a laser pulse in a thin surface liquid layer is discussed in detail. It has been shown that microorganisms transferred using the new bioprinting technology are viable and can be successfully cultivated on a dense nutrient medium.

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Extending Single Cell Bioprinting from Femtosecond to Picosecond Laser Pulse Durations

Cited by 9 publications

References 44 publications

Laser transfer for circulating tumor cell isolation in liquid biopsy

Laser transfer for circulating tumor cell isolation in liquid biopsy

Hydrogels and Bioprinting in Bone Tissue Engineering: Creating Artificial Stem‐Cell Niches for In Vitro Models

Laser bioprinting without donor plate

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