Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity

Lapidus, Alla; Goltsman, Eugene; Auger, Sandrine; Galleron, Nathalie; Ségurens, Béatrice; Dossat, Carole; Land, Miriam; Broussolle, Véronique; Brillard, Julien; Guinebretière, Marie-Hélène; Sanchis, Vincent; Nguen-the, Christophe; Lereclus, Didier; Richardson, Paul; Wincker, Patrick; Weissenbach, Jean; Ehrlich, S. Dusko; Sorokin, Alexeï

doi:10.1016/j.cbi.2007.03.003

Cited by 147 publications

(132 citation statements)

References 46 publications

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“…These findings open the way to new hypothetical mechanisms for the spread history of enterotoxins, such as horizontal gene transfer (HGT) in the B. cereus group. This is coherent with traces of HGT previously observed on the B. cereus genome around hbl genes (14) or with the existence of plasmids carrying enterotoxin-like genes (15,16). On this assumption, some phylogenetic groups might have failed to acquire cytK or hbl (for example, group VI for the cytK gene or subgroup III-2 for hbl), because they occupy very specific thermal niches unfavorable to cohabitation and exchange of genetic material with other phylogenetic groups.…”

supporting

confidence: 83%

Ability of Bacillus cereus Group Strains To Cause Food Poisoning Varies According to Phylogenetic Affiliation (Groups I to VII) Rather than Species Affiliation

et al. 2010

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Cytotoxic activity levels of culture filtrates and toxin distributions varied according to the phylogenetic group (I to VII) within the Bacillus cereus group, suggesting that these groups are of different clinical significance and are more suitable than species affiliations for determining food poisoning risk. A first-line, simple online tool (https://www.tools.symprevius.org/Bcereus/english.php) to assign strains to the different phylogenetic groups is presented.

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supporting

confidence: 83%

Ability of Bacillus cereus Group Strains To Cause Food Poisoning Varies According to Phylogenetic Affiliation (Groups I to VII) Rather than Species Affiliation

et al. 2010

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“…There were a further 2148 genes present in the total extended core of 3904 (17% of the total). These genes may be part of the core excluded by the gene-calling software or sequencing errors in one or more WGS genomes, or were lost in nodes of the B. cereus phylogeny undergoing genome reduction (such as the cytotoxic outgroup strain bce98) (Lapidus et al 2008). These figures for the core and pan-genome size concur with early estimates by Lapidus et al (2008) and Han et al (2006).…”

Section: Bgsc 4ba1supporting

confidence: 54%

“…These outlying strains also have a higher rate of homologous recombination relative to mutation ). The only one of these strains with a known association to pathogenicity is the food poisoning strain B. cereus cytotoxis NVH391-98 (bce98), which expresses a diarrheic Nhe hemolytic enterotoxin (Lapidus et al 2008). It is therefore possible that in this case these patterns of genomic change are not specifically associated with the phenotype of virulence for mammals.…”

Section: Discussionmentioning

confidence: 99%

Genomic characterization of theBacillus cereussensu lato species: Backdrop to the evolution ofBacillus anthracis

Zwick¹,

Joseph²,

Didelot³

et al. 2012

Genome Res.

155

139

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The key genes required for Bacillus anthracis to cause anthrax have been acquired recently by horizontal gene transfer. To understand the genetic background for the evolution of B. anthracis virulence, we obtained high-redundancy genome sequences of 45 strains of the Bacillus cereus sensu lato (s.l.) species that were chosen for their genetic diversity within the species based on the existing multilocus sequence typing scheme. From the resulting data, we called more than 324,000 new genes representing more than 12,333 new gene families for this group. The core genome size for the B. cereus s.l. group was ∼1750 genes, with another 2150 genes found in almost every genome constituting the extended core. There was a paucity of genes specific and conserved in any clade. We found no evidence of recent large-scale gene loss in B. anthracis or for unusual accumulation of nonsynonymous DNA substitutions in the chromosome; however, several B. cereus genomes isolated from soil and not previously associated with human disease were degraded to various degrees. Although B. anthracis has undergone an ecological shift within the species, its chromosome does not appear to be exceptional on a macroscopic scale compared with close relatives.

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“…To examine the cell wall binding ability of CwlB, the purified CwlB-His protein and purified B. thuringiensis Cry1Ie-His protein (49,50), which is an endotoxin with no ability to bind with cell walls and was used as the negative control, were added into B. thuringiensis cell wall buffer with a final absorbance of 0.3 at 540 nm. CwlB was also used in the absence of cell walls as another control to test whether CwlB can sediment.…”

Section: Sequence Analysis and Transcriptional Units In The Bt2492-btmentioning

confidence: 99%

Transcriptional Regulation and Characteristics of a Novel N -Acetylmuramoyl- l -Alanine Amidase Gene Involved in Bacillus thuringiensis Mother Cell Lysis

et al. 2013

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cIn Bacillus thuringiensis, a novel N-acetylmuramoyl-L-alanine amidase gene (named cwlB) was detected, and the CwlB protein was purified and characterized. Reverse transcription-PCR (RT-PCR) results indicated that cwlB and an upstream gene (named cwlA) formed one transcriptional unit. 5= rapid amplification of cDNA ends (5=-RACE)-PCR and transcriptional fusions with the lacZ gene indicated that transcription of the operon was directed by a promoter, P cwlA , which is located upstream from the cwlA gene and that the transcription start site is a single 5=-end nucleotide residue T located 25 nucleotides (bp) upstream from the cwlA translational start codon. Moreover, the activity of P cwlA was controlled by K . Morphological analysis suggested that the mutation of cwlB could delay spore release compared to the timing of spore release in the wild-type strain. Western blot assay demonstrated that purified CwlB bound to the B. thuringiensis cell wall. Observations with laser confocal microscopy and a green fluorescent protein-based reporter system demonstrated that the CwlB protein localizes to the cell envelope. All results suggest that the CwlB protein is involved in mother cell lysis in B. thuringiensis.

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Extending the Bacillus cereus group genomics to putative food-borne pathogens of different toxicity

Cited by 147 publications

References 46 publications

Ability of Bacillus cereus Group Strains To Cause Food Poisoning Varies According to Phylogenetic Affiliation (Groups I to VII) Rather than Species Affiliation

Ability of Bacillus cereus Group Strains To Cause Food Poisoning Varies According to Phylogenetic Affiliation (Groups I to VII) Rather than Species Affiliation

Genomic characterization of theBacillus cereussensu lato species: Backdrop to the evolution ofBacillus anthracis

Transcriptional Regulation and Characteristics of a Novel N -Acetylmuramoyl- l -Alanine Amidase Gene Involved in Bacillus thuringiensis Mother Cell Lysis

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