Extension of the equilibration period up to 24 h maintains the post-thawing quality of Holstein bull semen frozen with OPTIXcell®

Salman, Amer; Fernández‐Alegre, Estela; Francisco-Vázquez, Rubén; Gómez-Martín, Rubén; Fernández-Fernández, Alejandro; Areán-Dablanca, Héctor; Domínguez, Juan Carlos; González-Montaña, José-Ramiro; Caamaño, J. N.; Martínez‐Pastor, Felipe

doi:10.1016/j.anireprosci.2023.107209

Cited by 8 publications

(9 citation statements)

References 30 publications

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“…Therefore, the aforementioned authors found that changes in laboratory parameters or fertility with different extenders did not depend on the bull used. In contrast, Salman et al [15] found that the change with different equilibration times significantly depended on the individual bull. To our knowledge, using a bull-specific extender for 24 h equilibration time in our study was a new approach.…”

Section: Discussionmentioning

confidence: 89%

“…Similar to our study, Fleisch et al [7] found only numerically better sperm motility when using OptiXcell for 24 h compared to 4 h equilibration. Others found a small decrease in progressive motility directly after thawing, but not after 4 h of incubation, when using 24 h equilibration compared to 4 h with OptiXcell [15]. The aforementioned authors also found significantly better plasma membrane integrity [7] and chromatin structure with no effects on oxidative stress, or on apoptotic or capacitation markers [15].…”

Section: Discussionmentioning

confidence: 96%

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Influence of Equilibration Time and Bull-Specific Extender for Cryopreservation on Semen Quality and Fertility in German Holstein Friesian Bulls: A Controlled Field Trial

Pieper,

Meschede,

Jung

et al. 2023

Animals

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Equilibration with an extender is necessary to allow cryopreservation of bovine sperm. The aim of trial 1 was to assess the effect of 24 h versus 4 h equilibration time with three different extenders on sperm quality and to select the preferred extender for each bull. The aim of trial 2 was to investigate the effect of using a 24 h equilibration time with a bull-specific extender on field fertility. For trial 1, three ejaculates each from eight Holstein Friesian breeding bulls were used as the split-sample, including two equilibration times (4 h and 24 h) and three extenders (BioXcell, Triladyl, and OptiXcell). For trial 2, from 5 to 10 ejaculates from the same bulls were collected and treated (split-sample) as BioXcell with 4 h equilibration and either Triladyl or OptiXcell, both with 24 h equilibration. A total of 11,059 straws were used for insemination of cows and heifers. For Triladyl, progressive sperm motility, acrosome defects, and plasma membrane and acrosome integrity improved with a 24 h compared to a 4 h equilibration time. Four bulls each were used with Triladyl and OptiXcell for trial 2. In trial 2, non-return rates did not differ among groups. Therefore, using a 24 h equilibration time might improve in vitro sperm parameters, depending on the extender used. Moreover, it would be possible to change from 4 h to 24 h equilibration time without impairing field fertility.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 96%

Influence of Equilibration Time and Bull-Specific Extender for Cryopreservation on Semen Quality and Fertility in German Holstein Friesian Bulls: A Controlled Field Trial

Pieper,

Meschede,

Jung

et al. 2023

Animals

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“…Many studies have focused on the use of liposomes as cryoprotectant additives during the cryopreservation of animal semen to limit the freezing–thawing-induced damages and improve breeding aims. For this purpose, it can be mentioned that a commercial extender based on liposomes (OptiXcell ® ) is available, and it is used in the cryopreservation of sperm from several animal species [ 10 , 43 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Effects of Charged and Zwitterionic Liposomes on Human Spermatozoa and Supplementation with Liposomes and Chlorogenic Acid during Sperm Freezing

Moretti,

Bonechi,

Signorini

et al. 2024

Cells

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Semen handling and cryopreservation induce oxidative stress that should be minimized. In this study, human semen was supplemented during cryopreservation with formulations of handmade liposomes and chlorogenic acid (CGA), an antioxidant compound. Zwitterionic (ZL), anionic (AL), and cationic (CL) liposomes were synthesized and characterized. Three aliquots of swim-up-selected sperm were incubated with ZL, AL, and CL (1:10,000), respectively. The percentages of sperm with progressive motility, high mitochondrial membrane potential (MMP; JC-1), double-stranded DNA (dsDNA acridine orange), and acrosome integrity (Pisum sativum agglutinin) were assessed. Then, human semen was frozen using both 1:10,000 ZL and CGA as follows: freezing medium/empty ZL (EL), freezing medium/empty ZL/CGA in the medium (CGA + EL), freezing medium/CGA loaded ZL (CGA), freezing medium (CTR). The same sperm endpoints were evaluated. ZL were the most tolerated and used for semen cryopreservation protocols. All the supplemented samples showed better endpoints versus CTR (p < 0.001). In particular, spermatozoa from the CGA and CGA + EL A samples showed increased motility, dsDNA, and acrosome integrity versus CTR and EL (p < 0.001; motility EL vs. CGA + EL p < 0.05). ZL and CGA can improve post-thaw sperm quality, acting on both cold shock effect management and oxidative stress. These findings open new perspectives on human and animal reproduction.

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“…В настоящее время разработано большое количество криопротекторных сред для успешного проведения процедуры криоконсервации спермы (OptiXcell, BioXcell, BullXcell, INRA96, NutriXcell и AndroMed, CEP-2) [11]. В состав различных разбавителей могут входить: соевый лецитин (AndroMed), свежий яичный желток (BullXcell), плазма каудального придатка семенника (CEP-2) [5].…”

Section: результаты и обсуждениеunclassified

“…Разбавитель компании IMV(Франция) включает липосомы и не содержит в своем составе животного белка. Концентрированная средаразбавитель неоднократно использовалась в различных исследованиях [9,11]. Chaudhari D.V., Dhami A. J.…”

Section: результаты и обсуждениеunclassified

Results of testing the protocol for cryopreservation of bull sperm using a concentrated dilutent medium

Korochkina,

Pushkina,

Bakhturina

et al. 2024

jour

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In recent years, dairy farming has been actively developing in Russia in many directions, providing consumers with milk, dairy products, meat and other raw materials. Particularly relevant are the automation of the processes of raising cattle and the selection of the most economically profitable dairy and meat breeds, which is facilitated by the development of artificial insemination in this agricultural sector. The success of artificial insemination is determined by many factors, including the quality of sperm extenders used and the correctness of cryopreservation protocols. The purpose of this study is to test a protocol for cryopreservation of Holstein bull sperm. The ejaculate was collected, evaluated, and pre-prepared (centrifugation, dilution, cooling). The freezing process was carried out using the method presented in the article by AnsariM.S., RakhaB. A. et al. (2015) for buffalo sperm: the straws were kept above liquid nitrogen vapor at a distance of 5 cm for 10 minutes, and then immersed in a Dewar flask. Sperm motility was assessed 3 times: after sperm collection, after cooling, and 0 hours after thawing. The negative impact of the cryopreservation process on the morphofunctional characteristics of sperm was determined. The study noted a decrease in the motility of progressively moving sperm by 21.53%, as well as a sharp increase in immobile sperm before and after cryopreservation from 6.8% to 33.82%. The number of nonprogressive spermatozoa was reduced from 9.12% to 2.91%. Changes in the morphological parameters of sperm as a result of cryopreservation (after cooling and 0 hours after thawing): the number of normal sperm decreased by 12%, the number of sperm with tail defects increased by 11% and head by 2%. Based on the data obtained, we can conclude that the use of this cryopreservation protocol, with preliminary preparation, helps preserve sperm viability. Further research will be aimed at studying and creating cryoprotectors that increase the safety of sperm of breeding bulls during deep freezing.

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Extension of the equilibration period up to 24 h maintains the post-thawing quality of Holstein bull semen frozen with OPTIXcell®

Cited by 8 publications

References 30 publications

Influence of Equilibration Time and Bull-Specific Extender for Cryopreservation on Semen Quality and Fertility in German Holstein Friesian Bulls: A Controlled Field Trial

Influence of Equilibration Time and Bull-Specific Extender for Cryopreservation on Semen Quality and Fertility in German Holstein Friesian Bulls: A Controlled Field Trial

In Vitro Effects of Charged and Zwitterionic Liposomes on Human Spermatozoa and Supplementation with Liposomes and Chlorogenic Acid during Sperm Freezing

Results of testing the protocol for cryopreservation of bull sperm using a concentrated dilutent medium

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