1996
DOI: 10.1128/mcb.16.3.745
|View full text |Cite
|
Sign up to set email alerts
|

Extensive Alternative Splicing and Dual Promoter Usage Generate Tcf-1 Protein Isoforms with Differential Transcription Control Properties

Abstract: Previously, we reported the isolation of cDNA clones representing four alternative splice forms of TCF-1, a T-cell-specific transcription factor. In the present study, Western blotting (immunoblotting) yielded a multitude of TCF-1 proteins ranging from 25 to 55 kDa, a pattern not simply explained from the known splice alternatives. Subsequent cDNA cloning, PCR amplification, and analysis by rapid amplification of 5 cDNA ends revealed (i) the presence of an alternative upstream promoter, which extended the know… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1
1

Citation Types

3
182
3
2

Year Published

1997
1997
2014
2014

Publication Types

Select...
9
1

Relationship

0
10

Authors

Journals

citations
Cited by 181 publications
(190 citation statements)
references
References 22 publications
3
182
3
2
Order By: Relevance
“…This indicates a possible role for the Wnt-pathway in ovarian tumour cells. Several members of the Tcf-Lef family are expressed in colorectal cancer cells and malignant mammary epithelium (Van de Wetering et al, 1996). Nuclear staining for b-catenin and Lef-1, indicating dysfunction in normal Wnt-signalling, was found in the OVCAR-3 cells but not in ovarian tumour biopsies.…”
Section: Discussionmentioning
confidence: 96%
“…This indicates a possible role for the Wnt-pathway in ovarian tumour cells. Several members of the Tcf-Lef family are expressed in colorectal cancer cells and malignant mammary epithelium (Van de Wetering et al, 1996). Nuclear staining for b-catenin and Lef-1, indicating dysfunction in normal Wnt-signalling, was found in the OVCAR-3 cells but not in ovarian tumour biopsies.…”
Section: Discussionmentioning
confidence: 96%
“…In mice, Tcf-3 represses Wnt/ -catenin signaling either through a competitive physical interaction withcatenin or via competition for Tcf/Lef binding sites on DNA [396,398]. Further diversity of family members may be created by alternative splicing [399].…”
Section: The -Catenin Tcf/lef Transcription Complexmentioning
confidence: 99%
“…When β-catenin translocates to the nucleus, it binds to the β-catenin-binding domain of TCF/LEF and activates transcription of target genes. The TCF/LEF family has several spliced forms with unknown functional significance and it is suggested that they may activate preferential genes [86] . In HCC, mutations of TCF-4 are rare with 315 August 10, 2011|Volume 2|Issue 8| WJCO|www.wjgnet.com [70][71][72] phospho-GSK3β High 52 IHC [73] Axin1 Low 67 IHC [76] Dvl High 71 Western blotting [82] Prickle-1 Low 55 PCR [82] HDPR1 Low 58 PCR [83] PIN1 High 53 Western blotting [65] TCF-4 High 91 PCR [87] LEF-1 High 52 IHC [88] CDH17 High 72 IHC [90] CDH17: Cadherin-17; Dvl: Dishevelled; FZD: Frizzled; phospho-GSK3β: Phosphorylated glycogen synthase kinase 3β; HDPR1: Human homologue of Dapper; IHC: Immunohistochemistry; LEF: Lymphoid enhancer factor; PCR: Polymerase chain reaction; PIN1: Peptidyl-prolyl cis/trans isomerase; TCF: T-cell factor.…”
Section: Tcf/lefmentioning
confidence: 99%