Extensive analysis of different allelelic structures of the chicken BF2 and β2m proteins

Li, Xin Sheng; Qin, Fang; Yan, Ruo Qian; Gao, Feng; He, Hao; Jia, Zhen; Lin, Chang; Xia, Chun

doi:10.1016/j.vetimm.2006.03.023

Cited by 3 publications

(2 citation statements)

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“…During the last several years, the two-dimensional (2D) and three-dimensional (3D) structures of chb2M and MHC class I proteins have been frequently discussed. As originally noted, (8) chb2M contains a typical b-sheet with no a-helix. Although the 20 amino acid residues of the C-terminus of chb2M consist of two discontinuous b-sheets, they may be freely exposed to the external environment.…”

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confidence: 67%

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Monoclonal Antibodies Directed Against Chicken β2-Microglobulin Developed With a Synthesized Peptide

Chen

Qiu

Qian

et al. 2013

Monoclonal Antibodies in Immunodiagnosis and Immunotherapy

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We developed a panel of monoclonal antibodies (MAb) against chicken β2-microglobulin (chβ2M) by fusions between SP2/0 myeloma cells and spleen cells from mice immunized with a synthesized peptide corresponding to positions 91-119 of the COOH domain of chβ2M. Two of them, 6E7 and 3D1, identified as IgG1/κ, could react with chβ2M protein from avian macrophage HD11 cells and human 293T cells transfected with pcDNA3.1-chβ2M in immunofluorescence assays. Only a 12 kDa protein band of chβ2M could be detected in the HD11 and 293T/chβ2M cell lysates by Western blot analysis. Chicken β2M in serum and plasma could be found in Western blot by MAb 3D1. Moreover, MAb 3D1 also recognized the chβ2M antigen on the cell membranes in flow cytometry. Immunohistochemical staining with these MAbs revealed that chβ2M was present in chicken thymus, spleen, and bursa. These MAbs will be good tools for analyzing the mechanism of the chicken immune system.

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confidence: 67%

“…(3,6) However, there are only a few reports focusing on chicken b2-microglobulin (chb2M). (7)(8)(9)(10) In this study, we developed a panel of monoclonal antibodies against chb2M with a synthesized peptide. These monoclonal antibodies could react with both linear chb2M and native chb2M.…”

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confidence: 99%