Extensive analysis of the cultivated medicinal herbal drug Origanum dictamnus L. and antimicrobial activity of its constituents

Paloukopoulou, Charikleia; Tsadila, Christina; Govari, Sofia; Soulioti, Athina; Mossialos, Dimitris; Karioti, Anastasia

doi:10.1016/j.phytochem.2023.113591

Cited by 10 publications

(6 citation statements)

References 30 publications

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“…Based on the phytochemical screening by LC/MS, the OdLe was particularly rich in flavonoids but also in phenolic acids, such as curcumin, kampferol, silymarin, cyanidin-3-glucoside, deosmin, rutin, tartric acid, coumaric acid, and quercetin. This finding is in line with previous reports [58,59]. In addition, this outcome enhances the high antioxidant content of OdLe, as it has been reported that the main biological activity of flavonoids is their antioxidant activity [60].…”

Section: Discussionsupporting

confidence: 93%

Antioxidant Profile of Origanum dictamnus L. Exhibits Antiaging Properties against UVA Irradiation

Letsiou,

Trapali,

Vougiouklaki

et al. 2023

Cosmetics

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Skin aging mainly occurs due to intrinsic and extrinsic factors. Extrinsic aging is a consequence of exposure to ultraviolet radiation. Meanwhile, natural products exhibit protective properties against skin aging as well as photoaging. In this context, the research on natural anti-aging agents is greatly advanced, and in recent years, numerous plant-based products have been investigated. The aim of this study was to assess the antioxidant profile of Origanum dictamnus L. extract as well as its antiaging effects on 2D cultures of fibroblasts and keratinocytes under UVA irradiation to unravel the potential role of Origanum dictamnus L. in cosmetology. In an attempt to explore the antioxidant profile of the extract, we employed well-established enzymatic assays (DPPH, FRAP, ABTS, and TPC) and a phytochemical screening by LC/MS. According to our findings, the Origanum dictamnus L. extract possesses high scavenging activity (DPPH, ABTS), high phenolic content (TPC), and high Fe(III)-reduction activity (FRAP). Moreover, the LC/MS analysis revealed that the extract was rich in flavonoids, holding a high content of curcumin, kampferol, silymarin, cyanidin-3-glucoside, deosmin, rutin, and quercetin. To gain insight into the bioactivity of Origanum dictamnus L. extract in cell aging, the expression of various genes that are implicated in the skin aging process in keratinocytes and fibroblasts was studied. The gene expression analysis revealed that the extract increases cell proliferation in the cells exposed to UVA irradiation and concomitantly modulates the expression of genes related to the aging process in keratinocytes (KLK7, OCLN, GBA1) and fibroblasts (SIRT2, FOXO3, COL3A1) under the same conditions.

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Section: Discussionsupporting

confidence: 93%

Antioxidant Profile of Origanum dictamnus L. Exhibits Antiaging Properties against UVA Irradiation

Letsiou,

Trapali,

Vougiouklaki

et al. 2023

Cosmetics

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“…Finally, two more flavonoids 14 and 17 were identified as acacetin 7-O-rhamnosylhexoside and acacetin, respectively, based on HPLC-PDA-MS data and previous reports on closely related genera such as Micromeria and Calamintha, belonging to genus Satureja sensu lato [41]. Their identification (Table 1) was based on co-chromatography with reference standards and lab isolates [25,37], as well as the literature data [38][39][40][41]. The following compounds were identified: 12-hydroxyjasmonic acid glucoside (1), vicenin 2 (2); luteolin 7-O-diglucoside (4), luteolin 7-O-rutinoside (5), luteolin 7-O-glucoside (6), luteolin 7-O-glucuronide (7), hesperidin (hesperitin 7-O-rutinoside) (8), luteolin 3 -O-glucuronide (10), an unidentified depside tetramer (11), acacetin 7-O-rhamnosylhexoside ( 14), 5,6,3trihydroxy-7,8,4-trimethoxyflavone (16) and acacetin (4 -methylapigenin) (17).…”

Section: Isolation Of the Main/characteristic Constituentsmentioning

confidence: 96%

“…HPLC-PDA-MS analyses of the crude extract (Figure 2) and fractions thereof enabled the elucidation of the rest of the constituents. Their identification (Table 1) was based on co-chromatography with reference standards and lab isolates [25,37], as well as the literature data [38][39][40][41]. The following compounds were identified: 12-hydroxyjasmonic acid glucoside (1), vicenin 2 (2); luteolin 7-O-diglucoside (4), luteolin 7-O-rutinoside (5), luteolin 7-O-glucoside ( 6), luteolin 7-O-glucuronide (7), hesperidin (hesperitin 7-O-rutinoside) (8), luteolin 3′-O-glucuronide (10), an unidentified depside tetramer (11), acacetin 7-O-rhamnosylhexoside ( 14), 5,6,3′-trihydroxy-7,8,4-trimethoxyflavone (16) and acacetin (4′-methylapigenin) (17).…”

Section: Isolation Of the Main/characteristic Constituentsmentioning

confidence: 99%

HPLC- and NMR-Based Chemical Profiling, Wound-Healing Potential, Anti-Inflammatory and Antibacterial Activities of Satureja pilosa (Lamiaceae), a Neglected Medicinal–Aromatic Herb

Panagiotidou,

Burgers,

Tsadila

et al. 2023

Plants

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Satureja pilosa Velen. (Lamiaceae) is a perennial and melliferous aromatic–medicinal subshrub which is range-restricted in adjacent parts of Greece and Bulgaria and locally in Italy, known in Northern Greece as wild oregano (“agriorigani”) and traditionally collected from the wild for culinary purposes. Since the ethnopharmacological data and modern biological activities of Satureja spp. suggest promising applications in skin conditions, the present study aimed to investigate the hitherto unknown phenolic content of cultivated S. pilosa and its potential biological activities, focusing mainly on wound-healing and anti-inflammatory effects. An HPLC-PDA-MS-targeted phytochemical investigation, along with NMR, allowed for the isolation and characterization of the main constituents, resulting in 18 compounds. Representative extracts and purified compounds were tested for wound-healing activity on NIH/3T3 fibroblasts. The butanol extract exhibited a significantly higher cell migration rate (73.4%) compared to aqueous (50.6%) and methanolic (49.6%) ones, enhancing the cell migration more rapidly at both concentration levels, whilst rosmarinic acid was the most potent among the isolated compounds, with a migration rate of 64.0% at the concentration level of 10−5 mg/mL, followed by 3,4-dihydrophenyllactic acid (54.7%). Moreover, potential effects on endothelial activation processes were explored, including the leukocyte–endothelial cell interaction during inflammatory processes and the migratory capacity during angiogenic actions, since these processes are commonly associated with skin diseases. Finally, extracts and purified compounds demonstrated weak antibacterial potential against two important pathogens (Staphylococcus aureus and Pseudomonas aeruginosa), suggesting that further investigation is warrented.

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“…It is suggested to be consumed following a potion containing earthworms as its primary ingredient, purportedly to alleviate dysuria [54] (pp. [129][130][131]. However, its diuretic properties are also noted to have been recognized as early as the 5th century BCE by Euryphonas cited by Soranus [48] and reiterated by the physician Rufus in the late first or early second century CE [55].…”

Section: Dittany Through Ancient Greek Authorsmentioning

confidence: 99%

Ecology, Cultivation, and Utilization of the Dittany of Crete (Origanum dictamnus L.) from Ancient Times to the Present: A Short Review

Solomou,

Fountouli,

Molla

et al. 2024

Agronomy

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Medicinal and aromatic plants are a consistent component of the biodiversity heritage in numerous countries worldwide. Origanum dictamnus L. (Lamiaceae family), also known as Dittany, an endemic plant of the Greek island of Crete, has been widely used as traditional medicine since antiquity, all over Europe. The aim of the present review is to provide a thorough and detailed account of Dittany in antiquity, the plant’s physical characteristics and ecology, and its cultivation methods, as well as its chemical components, biological properties, and pharmacological studies. The information is presented and analyzed in a critical manner. A total of 86 research studies were systematically reviewed based on the Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) guidelines. The findings indicate that Dittany is one of the most important medicinal and aromatic plants, with many uses not only in pharmacology but also in gastronomy. While a large body of literature exists regarding the application of essential oils, the number of publications concerning the plant’s cultivation is rather small. Therefore, the main focus of this review is on the cultivation methods and the significance of cultivating and employing Dittany in Greece and the wider Mediterranean region in the future. Further research on this plant species is warranted since it has significant medicinal, economic, and environmental value.

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Extensive analysis of the cultivated medicinal herbal drug Origanum dictamnus L. and antimicrobial activity of its constituents

Cited by 10 publications

References 30 publications

Antioxidant Profile of Origanum dictamnus L. Exhibits Antiaging Properties against UVA Irradiation

Antioxidant Profile of Origanum dictamnus L. Exhibits Antiaging Properties against UVA Irradiation

HPLC- and NMR-Based Chemical Profiling, Wound-Healing Potential, Anti-Inflammatory and Antibacterial Activities of Satureja pilosa (Lamiaceae), a Neglected Medicinal–Aromatic Herb

Ecology, Cultivation, and Utilization of the Dittany of Crete (Origanum dictamnus L.) from Ancient Times to the Present: A Short Review

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