Extensive colonization of the porcine colonic epithelium by a spirochete similar to Treponema innocens

Jacques, Mario; Girard, Christiane; Higgins, Robert; Goyette, Gabriel C.

doi:10.1128/jcm.27.5.1139-1141.1989

Cited by 35 publications

(26 citation statements)

References 10 publications

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“…The in vitro attachment of S. hyodysenteriae to epithelial cells depends on the motility and viability of the spirochaetes and that spirochaetal attachment is inhibited by convalescent antisera (3). End-on attachment of densely packed perpendicularly orientated spirochaetes to the apical surface of the colonic epithelium like a false brush border has been reported for S. pilosicolin infections in pigs (13,26). Similar end-on attachment of intestinal spirochaetes to colonic epithelium is well known in other domestic animals as well as in humans (25,27).…”

Section: Discussionsupporting

confidence: 56%

“…Thus, the extension of S. hyodysenteriae end-on attachment to the epithelium remains to be determined. Extensive end-on attachment of other intestinal spirochaetes to the colonic epithelium has been observed in pigs and other animals as well as in humans (8, 13,25,26,27). Detection of spirochaetes in tissue samples using different methods, including direct microscopy and e.g.…”

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confidence: 99%

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Association of Serpulina hyodysenteriae with the colonic mucosa in experimental swine dysentery studied by fluorescent in situ hybridization

Jensen¹,

Boye²,

Möller³

et al. 1998

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Association of Serpulina hyodysenteriae with the colonic mucosa in experimental swine dysentery studied by fluorescent in situ hybridization.The localization of Serpulina hyodysenteriae in experimental swine dysentery was studied by fluorescent in situ hybridization (FISH) using an oligonucleotide probe targeting the 23s rRNA of S. hyodysenteriae. Nine 8-week-old pigs were challenged. Seven of the pigs were intragastrically dosed with 1 X lo9 cfu S. hyodysenteriae for 3 consecutive days, whereas two pigs were infected by contact. Six non-challenged pigs served as negative controls. The challenged pigs developed clinical swine dysentery from 8 to 14 days postinfection with typical gross lesions. By FISH S. hyodysenteriae cells in huge numbers were found colonizing the mucus layer, the luminal epithelium and the crypts of the large intestinal mucosa. The orientation of the spirochaetes to the epithelium appeared to be random. Spirochaetes in clusters or as single cells were invading the epithelium and were occasionally detected in the adjacent lamina propria. The distribution of spirochaetes in the mucosa provides further evidence that S. hyodysenteriae is intimately associated with the mucus layer and the epithelium in a random pattern. Furthermore, the results demonstrate the applicability of FISH for specific detection of S. hyodysenteriae cells in clusters or as single cells in formalin-fixed tissue samples.

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Section: Discussionsupporting

confidence: 56%

mentioning

confidence: 99%

Association of Serpulina hyodysenteriae with the colonic mucosa in experimental swine dysentery studied by fluorescent in situ hybridization

Jensen¹,

Boye²,

Möller³

et al. 1998

APMIS

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“…Pigs suffering from spirochetal diarrhea usually show milder clinical signs than pigs with swine dysentery. Histologically, the condition is characterized by a false brush border in the large intestine, with end-on attachment of spirochetes to the epithelium in the cecum and colon (13,23). The histological findings suggest that the pathogenesis differs from that of swine dysentery, in which the false brush border is absent and the epithelial damage is more severe (11).…”

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confidence: 99%

Identification of Serpulina species associated with porcine colitis by biochemical analysis and PCR

et al. 1997

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A PCR system for the detection and identification of group IV spirochetes (Serpulina pilosicoli) was designed to complement biochemical tests, e.g., the hippurate hydrolysis and ␤-glucosidase tests, and to verify the accuracy of a previously proposed biochemical classification system. The PCR assay was based on amplification of a segment of the 16S rRNA gene. Both primers were constructed to selectively amplify the 16S rRNA gene of Serpulina pilosicoli. All analyzed Serpulina strains exhibiting the capacity to hydrolyze hippurate and lacking ␤-glucosidase activity, including the type strain for spirochetal diarrhea, P43, were amplified with the PCR system. All other tested strains, including type and field strains of different phenotypes of Serpulina species, as well as Salmonella species, Campylobacter species, and Escherichia coli strains, were negative in the assay. Among the tested strains were 18 Scottish field isolates originating from the mucosae of pigs with colitis. A simple classification scheme, suitable for routine classification of porcine intestinal spirochetes, is also presented. The scheme is based on hemolysis, indole production, and the hippurate hydrolysis test.

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“…This condition was termed porcine intestinal spirochetosis or spirochetal diarrhea and was characterized by end-on attachment of spirochetes to the colonic epithelium. Subsequently, other researchers have shown an association between colonization of the porcine intestinal tract with certain weakly hemolytic spirochetes and the occurrence of diarrhea (1,6,14,27,30).…”

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confidence: 99%

Pathogenicity of human and porcine intestinal spirochetes in one-day-old specific-pathogen-free chicks: an animal model of intestinal spirochetosis

1995

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Extensive colonization of the porcine colonic epithelium by a spirochete similar to Treponema innocens

Cited by 35 publications

References 10 publications

Association of Serpulina hyodysenteriae with the colonic mucosa in experimental swine dysentery studied by fluorescent in situ hybridization

Association of Serpulina hyodysenteriae with the colonic mucosa in experimental swine dysentery studied by fluorescent in situ hybridization

Identification of Serpulina species associated with porcine colitis by biochemical analysis and PCR

Pathogenicity of human and porcine intestinal spirochetes in one-day-old specific-pathogen-free chicks: an animal model of intestinal spirochetosis

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