Extensive Cross Reactivity between &lt;i&gt;Treponema pallidum&lt;/i&gt; and Cultivable Treponemes Demonstrated by Sequential Immunoadsorption

Wos, Susan M.; Wicher, Konrad

doi:10.1159/000233987

Cited by 13 publications

(4 citation statements)

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“…In the present report, the preparation of the immune fractions and their immunological and molecular characterizations differed from those of previous studies. The TPI-IgG fraction contained antibodies in quantities and specificities (15,17,33,39,45,47, and 97 kDa; Fig. 1) similar to those previously shown in a fully resistant host after the removal of cross-reacting specificities (31,32).…”

Section: Discussionsupporting

confidence: 69%

“…Molecular analysis of the sera of recipients bled just before infection showed, in recipients of TPI-IgG ( Fig. 2A), antibodies active against T. pallidum peptides of 15,17,33,39,45, and 47 kDa. The reaction against TR must be due to natural antibodies present in the sera of the recipient.…”

Section: Resultsmentioning

confidence: 99%

“…Currently available data indicate that during the course of infection, there is an extensive production of cross-reacting treponemal antibodies (30)(31)(32)(33), and only a few antibodies are specific for T. pallidum (31,32). Attempts to confer protection by immunization with nonpathogenic treponemes or their antigenic fractions (for references, see reference 17) provided no protection to subsequent challenge with T. pallidum.…”

mentioning

confidence: 99%

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Effect of passive immunization with purified specific or cross-reacting immunoglobulin G antibodies against Treponema pallidum on the course of infection in guinea pigs

1992

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Whole immune serum or highly purified immunoglobulin G (IgG) antibodies to Treponema paUidum exhaustively adsorbed with three strains of nonpathogenic treponemes (TPI-IgG) were used for passive immunization of inbred strain 2 guinea pigs before and after intradermal challenge with 3.4 x 107 virulent T. pallidum Nichols organisms. Before challenge, control animals received a similarly purified IgG fraction containing either a cocktail of antibodies against three nonpathogenic treponemes (NPTI-IgG) or IgG prepared from normal guinea pig serum (NGPS-IgG). The purified fractions contained both IgGl and IgG2 isotypes. The antibody levels (detected by fluorescent treponemal antibody test and enzyme-linked immunosorbent assay) and molecular specificities (immunoblot) of sera obtained from recipient animals before infection reflected those of the purified fractions used for immunization. Three protocols of passive immunization were used. Whole immune serum containing specific and cross-reacting antibodies afforded better protection than TPI-IgG even though asymptomatic animals were not fully protected. A single intradermal injection (0.1 ml) of TPI-IgG or NPTI-IgG into one hind leg 22 h before infection at the same site provided relatively higher protection than multiple intravenous injections (total, 15 ml) of the respective individual preparations. Since purified NGPS-IgG injected in the same animals, into the opposite hind leg, failed to protect against the challenging infection, it is reasonable to assume that specific and cross-reacting antitreponemal antibodies of the IgGl subclass, which in guinea pigs are homocytotropic, play a relevant role in local protection.Natural and experimental infections with Treponema pal-* Corresponding author. t This article is dedicated to the memory of Thomas

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Section: Discussionsupporting

confidence: 69%

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

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Effect of passive immunization with purified specific or cross-reacting immunoglobulin G antibodies against Treponema pallidum on the course of infection in guinea pigs

1992

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“…Moreover, as previously reported (32), the size, duration, and severity of primary syphilitic lesions in C4D guinea pigs are substantially greater than those evoked by the same inoculum size in inbred strains 2 and 13. T. pallidum infection sensitizes the host not only against pathogenic treponemes but also against nonpathogenic treponemes (36) and rabbit proteins (34). It was considered appropriate, therefore, to include as controls T cells from guinea pigs immunized with T. phagedenis biotype Reiter or with T. pallidumfree testis fluid from infected rabbits (ITF; 35), neither of which was included in any of the previous studies.…”

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confidence: 99%

Adoptive transfer of immunity to Treponema pallidum Nichols infection in inbred strain 2 and C4D guinea pigs

Wicher

Jakubowski

et al. 1987

Infect Immun

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T lymphocytes purified from lymph nodes and spleens of chancre-immune, inbred strain 2 guinea pigs, when infused into syngeneic guinea pigs, conferred protection against challenge with Treponema pallidum subsp. pallidum Nichols. No protection was conferred by similar injections of cell suspensions from normal guinea pigs or guinea pigs immunized with T. phagedenis biotype Reiter or T. pallidum-free testis supernatants from infected rabbits. Similar results were obtained with homozygous C4D guinea pigs. After several months of infection, 2 of 11 strain 2 and 1 of 8 strain C4D recipients of T. pallidum-immune cells developed an erythematous reaction of short duration at the injection site; 2 of these recipients were positive for T. pallidum. Throughout the experimental period the humoral response to treponemal antigens was substantially lower in the adoptively immune guinea pigs than in various unprotected control groups. Passive immunity to infection with T. pallidum, however, seems to be dose related, since asymptomatic infection persisted for as long as 3 months after challenge in strain 2 guinea pigs transfused with 10(8) T. pallidum-immune lymphocytes, but not in C4D recipients of twice as many immune cells.

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Identity of Treponema pallidum subsp. pallidum polypeptides: Correlation of sodium dodecyl sulfate‐polyacrylamide gel electrophoresis results from different laboratories

Norris¹,

Alderete

Axelsen

et al. 1987

Electrophoresis

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Identity of Treponema pallidum subsp. pallidum polypeptides: Correlation of sodium dodecyl sulfatepolyacrylamide gel electrophoresis results from different laboratoriesAs the first step in a cooperative effort to standardize the identification of the polypeptides of Treponerna pallidum subsp. pallidurn, the sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) results obtained in 16 laboratories were compared. Although it was possible to correlate the positions of 16 ofthe major polypeptide bands, the cross-identification of many of the polypeptides was ambiguous, particularly in the low molecular weight range. Two-dimensional electrophoresis provided an improved means of separating and characterizing T.pallidum polypeptides as isolated molecular species. An approach to the unambiguous identification of treponemal polypeptides was outlined which will utilize two-dimensional electrophoresis in combination with specific properties attributable to individual proteins, including reactivity with monoclonal antibodies or monospecific antisera, biochemical and structural properties, and sequence information. To demonstrate the feasibility of this approach, two-dimensional electrophoresis in conjunction with immunoperoxidase staining was used to specifically identify three cloned T.pal1idum proteins. Abbreviations: CIE, crossed immunoelectrophoresis; 2DE, two-dimensional gel electrophoresis; IRS, infected rabbit serum; 2-ME, 2-mercaptoethanol; PBS, phosphate-buffered saline; RIP, radioimmunoprecipitation: SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis; T, Tween 20 0 VCH Verlagsgesellschaft mbH, D-6940 Weinheim, 1987 0173-0835/87/0202-0077 %02.50/0 S. J. Norris et al. Electrophoresis 1987,8, 77-92 Figure 16. Reactivity of rabbit antiserum directed against cloned antigenTmpC (1301, seeFig. 6)with the 2DEpatternof T.paNidum.Theprocedure wasas described in Fig. 15. (A) Reaction with the TmpC-specific antiserum. (B) Antigenic profile following IRS counterstaining. The antiserum reacted specifically with a single band on the SDS-PAGE pattern (far left) and a single spot at the acid end of the 2DE pattern.Figure 17. Localization of cloned antigen TpD (1301, see Fig. 6) in the 2DE profile of T.pallidum, utilizing a specific rabbit antiserum prepared by affinity chromatography. (A) Reactivity of TpD-specific antiserum. Due to prolonged incubation with the color reagent, background reactivities of the antiserum to TmpC and the major 61 polypeptide were also detectable in this case. (B) Antigenic profile following IRS counterstaining. The positions of TpD and TmpC in the 2D and single dimension SDS-PAGE profiles are indicated by the arrows.

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Extensive Cross Reactivity between <i>Treponema pallidum</i> and Cultivable Treponemes Demonstrated by Sequential Immunoadsorption

Cited by 13 publications

References 11 publications

Effect of passive immunization with purified specific or cross-reacting immunoglobulin G antibodies against Treponema pallidum on the course of infection in guinea pigs

Effect of passive immunization with purified specific or cross-reacting immunoglobulin G antibodies against Treponema pallidum on the course of infection in guinea pigs

Adoptive transfer of immunity to Treponema pallidum Nichols infection in inbred strain 2 and C4D guinea pigs

Identity of Treponema pallidum subsp. pallidum polypeptides: Correlation of sodium dodecyl sulfate‐polyacrylamide gel electrophoresis results from different laboratories

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