Extensive Diversity of Ionizing-Radiation-Resistant Bacteria Recovered from Sonoran Desert Soil and Description of Nine New Species of the Genus
            <i>Deinococcus</i>
            Obtained from a Single Soil Sample

Rainey, Fred A.; Keren, Ray; Ferreira, Margarida; Gatz, Bridget Z.; Nobre, Fernanda; Bagaley, Danielle; Rash, Brian A.; Park, MieJung; Earl, Ashlee M.; Shank, Nicole C.; Small, Alanna M.; Henk, Margaret C.; Battista, John R.; Kämpfer, Peter; Costa, Milton S. da

doi:10.1128/aem.71.11.7630.2005

Cited by 24 publications

(20 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Multiple-stress tolerance of the type strain Geodermatophilus obscurus was already described by Gtari et al [11], suggesting a correlation between tolerance profiles to desiccation, mitomycin C, hydrogen peroxide, and ionizing- and UV-radiation. Previous works of Rainey et al [15] and Giongo et al [16] already revealed the prevalence of IR resistant Geodermatophilus isolates from arid soil sample at comparatively the same radiation levels as observed for Deinococcus species and the predominance of species belongs to the family Geodermatophilaceae detected from intercontinental dust, illustrating, therefore, to resist radiation and desiccation stresses during travel in the high atmosphere.…”

Section: Introductionmentioning

confidence: 64%

Geodermatophilus poikilotrophisp. nov.: A Multitolerant Actinomycete Isolated from Dolomitic Marble

Montero‐Calasanz

Hofner

Göker

et al. 2014

BioMed Research International

View full text Add to dashboard Cite

A novel Gram-reaction-positive, aerobic actinobacterium, tolerant to mitomycin C, heavy metals, metalloids, hydrogen peroxide, desiccation, and ionizing- and UV-radiation, designated G18T, was isolated from dolomitic marble collected from outcrops in Samara (Namibia). The growth range was 15–35°C, at pH 5.5–9.5 and in presence of 1% NaCl, forming greenish-black coloured colonies on GYM Streptomyces agar. Chemotaxonomic and molecular characteristics of the isolate matched those described for other representatives of the genus Geodermatophilus. The peptidoglycan contained meso-diaminopimelic acid as diagnostic diaminoacid. The main phospholipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, and small amount of diphosphatidylglycerol. MK-9(H4) was the dominant menaquinone and galactose was detected as diagnostic sugar. The major cellular fatty acids were branched-chain saturated acids iso-C16:0 and iso-C15:0 and the unsaturated C17:1 ω8c and C16:1 ω7c. The 16S rRNA gene showed 97.4–99.1% sequence identity with the other representatives of genus Geodermatophilus. Based on phenotypic results and 16S rRNA gene sequence analysis, strain G18T is proposed to represent a novel species, Geodermatophilus poikilotrophi. Type strain is G18T (= DSM 44209T = CCUG 63018T). The INSDC accession number is HF970583. The novel R software package was used to compute the lethal doses with confidence intervals resulting from tolerance experiments.

show abstract

Section: Introductionmentioning

confidence: 64%

Geodermatophilus poikilotrophisp. nov.: A Multitolerant Actinomycete Isolated from Dolomitic Marble

Montero‐Calasanz

Hofner

Göker

et al. 2014

BioMed Research International

View full text Add to dashboard Cite

show abstract

“…By contrast, the other sequences were affiliated to Deinococcus hopiensis and D. navajonensis (95.5–99.8% similarity), two radioresistant strains recovered from arid soils in the Sonoran hot desert (Rainey et al. ). Four sequences grouped into OTU 13 were not closely related to any cultured strains, and their closest neighbor (94.1–95.2% similarity) was a 16S rRNA gene sequence detected in a quartz hypolith from the Acatama desert (Lacap et al.…”

Section: Resultsmentioning

confidence: 99%

Exploration of Deinococcus‐Thermus molecular diversity by novel group‐specific PCR primers

et al. 2013

View full text Add to dashboard Cite

The deeply branching Deinococcus-Thermus lineage is recognized as one of the most extremophilic phylum of bacteria. In previous studies, the presence of Deinococcus-related bacteria in the hot arid Tunisian desert of Tataouine was demonstrated through combined molecular and culture-based approaches. Similarly, Thermus-related bacteria have been detected in Tunisian geothermal springs. The present work was conducted to explore the molecular diversity within the Deinococcus-Thermus phylum in these extreme environments. A set of specific primers was designed in silico on the basis of 16S rRNA gene sequences, validated for the specific detection of reference strains, and used for the polymerase chain reaction (PCR) amplification of metagenomic DNA retrieved from the Tataouine desert sand and Tunisian hot spring water samples. These analyses have revealed the presence of previously undescribed Deinococcus-Thermus bacterial sequences within these extreme environments. The primers designed in this study thus represent a powerful tool for the rapid detection of Deinococcus-Thermus in environmental samples and could also be applicable to clarify the biogeography of the Deinococcus-Thermus phylum.

show abstract

“…2 mL ali quots of the cell suspension containing 10 8 cells/mL were exposed to UV radiation in an open sterile petri dish at a distance of 14 cm from a 254 nm UV source (CROSSLINKER CL E508.G) at intensity of 10 J/cm 2 h for total dose 25 J/cm 2 . The plates were wrapped with aluminum foil and incubated in the dark under appropriate condition for monitoring the sur vival of cells after 15 days of incubation at 30°C [10]. The sub cultured of isolated strains on TGY agar slant from primary screening were used as radioresistant strains for subsequent studies.…”

Section: Sample Collection Enrichment and Screening Proceduresmentioning

confidence: 99%

Isolation and characterization of a novel strain of genus Dietzia capable of multiple-extreme resistance

Gholami

Etemadifar

2015

Microbiology

View full text Add to dashboard Cite

Extensive Diversity of Ionizing-Radiation-Resistant Bacteria Recovered from Sonoran Desert Soil and Description of Nine New Species of the Genus Deinococcus Obtained from a Single Soil Sample

Cited by 24 publications

References 0 publications

Geodermatophilus poikilotrophisp. nov.: A Multitolerant Actinomycete Isolated from Dolomitic Marble

Geodermatophilus poikilotrophisp. nov.: A Multitolerant Actinomycete Isolated from Dolomitic Marble

Exploration of Deinococcus‐Thermus molecular diversity by novel group‐specific PCR primers

Isolation and characterization of a novel strain of genus Dietzia capable of multiple-extreme resistance

Contact Info

Product

Resources

About