Extensive miRNA expression analysis in craniopharyngiomas

Samis, Jill; Vanin, Elio F.; Bonaldo, Maria de Fátima; Tomita, Tadanori; Habiby, Reema; Zimmerman, Donald; Soares, Marcelo B.

doi:10.1007/s00381-016-3131-1

Cited by 11 publications

(8 citation statements)

References 56 publications

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“…Although the WISP2 gene mRNA was overexpressed in aCPs, the protein expression was ▶table 2 Expression of Wnt pathway genes in patients with adamantinomatous craniopharyngioma presenting controlled or progressive disease. absent in all samples, suggesting a post transcriptional modulation, probably by microRNAs, as recently described [19,31]. Contradictory findings have been described on WISP2 gene expression, since reduced expression has been observed in colorectal cancer [32] and increased expression in most adrenocortical tumors [33].…”

Section: Discussionsupporting

confidence: 60%

Impact of the Canonical Wnt Pathway Activation on the Pathogenesis and Prognosis of Adamantinomatous Craniopharyngiomas

et al. 2018

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mutations and abnormal β-catenin distribution are associated with the pathogenesis of adamantinomatous craniopharyngioma (aCP). We evaluated the expression of the canonical Wnt pathway components in aCPs and its association with mutations and tumor progression. Tumor samples from 14 aCP patients and normal anterior pituitary samples from eight individuals without pituitary disease were studied. Gene expression of Wnt pathway activator (), inhibitors (, ,, and ), transcriptional activator (), target genes (, , and,), and Wnt modulator () was evaluated by qPCR. β-Catenin, , and expression was determined by immunohistochemistry (IHC). The transcription levels of all genes studied, except , were higher in aCPs as compared to controls and mRNA levels correlated with mutation. mRNA was overexpressed in tumor samples of patients with disease progression in comparison to those with stable disease. β-Catenin was positive and aberrantly distributed in 11 out of 14 tumor samples. Stronger β-catenin immunostaining associated positively with tumor progression. positive staining was found in 10 out of 14 cases, whereas all aCPs were negative for. Wnt pathway genes were overexpressed in aCPs harboring mutations and in patients with progressive disease. Recurrence was associated with stronger staining for β-catenin. These data suggest that Wnt pathway activation contributes to the pathogenesis and prognosis of aCPs.

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Section: Discussionsupporting

confidence: 60%

Impact of the Canonical Wnt Pathway Activation on the Pathogenesis and Prognosis of Adamantinomatous Craniopharyngiomas

et al. 2018

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“…We observed lower levels of endogenous miR-596 in most melanoma samples compared to melanocytes and other tumor samples from patients (Figure 1a, 1b). Previous miRNA profiling studies showed that miR-596 is downregulated in oral cancer, bladder cancer, and craniopharyngioma (Olivieri et al, 2016;Samis et al, 2016).…”

Section: Discussionmentioning

confidence: 99%

miR-596 Modulates Melanoma Growth by Regulating Cell Survival and Death

Liu

Lin

et al. 2018

Journal of Investigative Dermatology

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Tumors grow because cancer cells lack the ability to balance cell survival and death signaling pathways. miR-596, a microRNA located at the 8p23.3 locus, has been shown by the TCGA-Assembler to be deleted in a significant number of melanoma samples. Here, we also validated the low levels of miR-596 in melanoma compared to tissue nevi, and Kaplan-Meier curve analysis revealed that low miR-596 expression was associated with worse overall survival. Moreover, we showed that miR-596 overexpression effectively inhibited MAPK/ERK signaling, cell proliferation, migration, and invasion and increased the cell apoptosis of melanoma cells. In addition, we found that miR-596 directly targets MEK1 and two apoptotic proteins, MCL1, and BCL2L1, in melanoma cells. Our findings indicated that miR-596 is an important miRNA that both negatively regulates the MAPK/ERK signaling pathway by targeting MEK1 and modulates the apoptosis pathway by targeting MCL1 and BCL2L1, suggesting that miR-596 could be a therapeutic candidate for treating melanoma, and a prognostic factor for melanoma patients.

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“…His drawings impressively depict, (nearly identical with modern photomicrographs) the typical components of ACP, for example, the palisading tumor cells providing at the margin of tumor mass adjacent to brain tissue, the loosely composed stellate reticulum cells as well as solid epithelial cells configured in a whorl‐like shape. Thanks to ongoing technical innovations in pathology in the domain of molecular analysis (Figure ) , the development of specific antibodies and new visualization modes, our understanding of ACP pathogenesis made a great leap forward. Important resultant knowledge of the activated pathways involved and their subcellular mapping are summarized in greater detail below.…”

Section: Characterization Of Acp Biology Exemplified By Histological mentioning

confidence: 99%

Models of human adamantinomatous craniopharyngioma tissue: Steps toward an effective adjuvant treatment

Hölsken

Buslei

2017

Brain Pathology

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Even though ACP is a benign tumor, treatment is challenging because of the tumor's eloquent location. Today, with the exception of surgical intervention and irradiation, further treatment options are limited. However, ongoing molecular research in this field provides insights into the pathways involved in ACP pathogenesis and reveal a plethora of druggable targets. In the next step, appropriate models are essential to identify the most suitable and effective substances for clinical practice. Primary cell cultures in low passages provide a proper and rapid tool for initial drug potency testing. The patient-derived xenograft (PDX) model accommodates ACP complexity in that it shows respect to the preserved architecture and similar histological appearance to human tumors and therefore provides the most appropriate means for analyzing pharmacological efficacy. Nevertheless, further research is needed to understand in more detail the biological background of ACP pathogenesis, which provides the identification of the best targets in the hierarchy of signaling cascades. ACP models are also important for the continuous testing of new targeting drugs, to establish precision medicine.

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Extensive miRNA expression analysis in craniopharyngiomas

Cited by 11 publications

References 56 publications

Impact of the Canonical Wnt Pathway Activation on the Pathogenesis and Prognosis of Adamantinomatous Craniopharyngiomas

Impact of the Canonical Wnt Pathway Activation on the Pathogenesis and Prognosis of Adamantinomatous Craniopharyngiomas

miR-596 Modulates Melanoma Growth by Regulating Cell Survival and Death

Models of human adamantinomatous craniopharyngioma tissue: Steps toward an effective adjuvant treatment

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