Extensive screening of cyclopeptide toxins in mushrooms by ultra-high-performance liquid chromatography coupled with quadrupole-Orbitrap mass spectrometry

Shan, Lei; Shi, Peiyu; Wu, Wenling; Xia, Bing; Fu, Xianpeng; Yue, Wan; Zhou, Yan

doi:10.1016/j.foodchem.2020.127146

Cited by 13 publications

(7 citation statements)

References 34 publications

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“…The typical instances were 15 Shenlingbaizhu ‐derived compounds ( 9 , 21 , 28 , 29 , 31 , 33 , 36 , 37 , 48 , 53 , 55 , 56 , 57 , 66 , and 75 , Figure 3) and mushroom‐derived cyclopeptide toxins. These cyclopeptide toxins have been recently detected by the UHPLC‐Q‐orbitrap MS/MS technology 54 . (2) Phytochemical work is ongoing, regardless of its over 200‐year history.…”

Section: Discussionmentioning

confidence: 99%

“…These cyclopeptide toxins have been recently detected by the UHPLC-Q-orbitrap MS/MS technology. 54 (2) Phytochemical work is ongoing, regardless of its over 200-year history. For example, the phytochemistry of For another two unexpected compounds scoparone (22) and pectolinarigenin (58), however, the implication of our discovery was not limited to phytochemical guidance.…”

Section: F I G U R Ementioning

confidence: 99%

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Proposing anti‐counterfeiting pharmacopoeia quality markers for Shenlingbaizhu granule based on UHPLC‐Q‐orbitrap‐MS identification

Zeng,

Li,

Cai

et al. 2023

Phytochemical Analysis

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IntroductionShenlingbaizhu granule, a Traditional Chinese Medicine prescription comprising Renshen, Gancao, and Shanyao, is widely consumed in China nowadays.ObjectiveThe study tries to propose pharmacopoeia quality markers (Q‐markers) to prevent counterfeiting involving Renshen, Gancao, and Shanyao.MethodologyA novel strategy, that is, library‐based ultra‐high‐performance liquid chromatography–quadrupole‐orbitrap mass spectrometry, was used to analyse the lyophilised aqueous powder of Shenlingbaizhu granule. Subsequently, quantum chemistry calculation and UV‐vis spectra scanning were also performed through theoretical or experimental approaches.ResultThirty‐two isomers have been strictly distinguished, especially positional isomeric isochlorogenic acid B versus isochlorogenic acid C, positional isomeric schaftoside versus isoschaftoside, positional isomeric ginsenoside Rg2 versus 20S‐ginsenoside Rg3, and stereoisomeric 20S‐ginsenoside Rg3 versus 20R‐ginsenoside Rg3. Seventeen compounds were unexpectedly observed, particularly scoparone and pectolinarigenin, while a total of 76 bioactive compounds have been putatively identified in the study. The quantum chemistry calculation and UV‐vis spectra scanning results revealed that glycyrrhizic acid, ginsenoside Re, ginsenoside Rb1, and diosgenin displayed different dipole moment values and maximum absorption wavelengths from each other.ConclusionThe study recommends glycyrrhizic acid, ginsenoside Re, ginsenoside Rb1, and diosgenin as four anti‐counterfeiting Q‐markers for the pharmacopoeia. The anti‐counterfeiting Q‐markers can be detected using conventional HPLC. The observation of 17 unexpected compounds updates our knowledge regarding the bioactives of Shenlingbaizhu granule.

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Section: Discussionmentioning

confidence: 99%

Section: F I G U R Ementioning

confidence: 99%

Proposing anti‐counterfeiting pharmacopoeia quality markers for Shenlingbaizhu granule based on UHPLC‐Q‐orbitrap‐MS identification

Zeng,

Li,

Cai

et al. 2023

Phytochemical Analysis

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“…Therefore, analytical methods have been developed to determine different types of mushroom toxins in mushrooms, serum or urine samples. Laboratory tests based on immunoassays [12,13] or HPLC [14] as well as LC-MS/MS [15,16] techniques are commonly used. LC-MS/MS, which offers high sensitivity and selectivity in the identification of toxin molecules based on their specific mass-to-charge ratio and retention times, has become the most widely used technology for the detection of mushroom toxins.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Multi-Class Mushroom Toxins in Mushroom and Biological Liquid Samples Using LC-MS/MS

Lu,

Zhang,

et al. 2024

Separations

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A comprehensive analytical method based on liquid chromatography–tandem mass spectrometry (LC-MS/MS) was developed for the simultaneous detection of 12 mushroom toxins (ibotenic acid, muscimol, muscarine, β-amanitin, α-amanitin, desoxoviroidin, γ-amanitin, phallisacin, illudin S, phallacidin, phalloidin and illudin M) in mushrooms, serum, urine and simulated gastric fluid. The samples were extracted with water or acetonitrile solution, and the serum sample was further purified with PSA sorbent. Chromatographic separation was performed on an ACQUITY UPLC HSS T3 column with gradient elution using methanol and water containing 1 mM ammonia fluoride as a mobile phase. Mass spectrometric acquisition was performed in electrospray positive ionization mode. Good linearities (R2 > 0.994) were obtained for 12 toxins over the range of 0.05~200 µg/L. Matrix-matched calibration curves were used for quantification. The method limits of quantification were 0.01~0.2 mg/kg for mushrooms and 0.15~2.0 µg/L for three biological liquid samples. The mean recoveries of 12 target toxins (spiked at three concentration levels) ranged from 73.0% to 110.3%, with relative standard deviations not exceeding 19.4%, which meets the requirements for the determination of trace compounds in a biological matrix. This method was applied to the analysis of mushroom samples from Yunnan Province. As a result, 11 toxins, not including illudin M, were detected with a concentration range of 0.61~2143 mg/kg.

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“…Mushrooms are widely distributed globally, some of which have high nutritional value and medicinal efficacy (Lei et al, 2020), but some of which are poisonous. There are more than 4,000 mushrooms species in China, among which 966-1,020 are edible (Dai et al, 2010;Wu et al, 2019), 692 are medicinal (Wu et al, 2019), and 435-480 are poisonous (Bau et al, 2014;Wu et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Identification of Gyromitra infula: A Rapid and Visual Method Based on Loop-Mediated Isothermal Amplification

Xie

Fan

et al. 2022

Front. Microbiol.

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With mushroom poisoning emerging as one of the most serious food safety problems worldwide, a rapid identification method of poisonous mushrooms is urgently required to investigate the source of poisoning. Gyromitra infula, a kind of poisonous mushroom, contains gyromitrin toxin, which causes epileptogenic neurotoxicity and hemolytic disease. This study aimed to establish a rapid and visual method of G. infula identification based on loop-mediated isothermal amplification (LAMP). A set of specific LAMP primers was designed, and its specificity in G. infula was confirmed against various mushroom species, including its closely related species and other macrofungi. The sensitivity assay showed that the minimum concentration of genomic DNA detected by LAMP was 1 ng/μl. The method’s applicability was conducted by preparing mushroom samples that were boiled and digested in artificial gastric juice. The results showed that the content as low as 1% G. infula can be successfully detected. This method can be completed within 90 min, and the reaction results can be directly observed by the naked eyes. Hence, the identification method of G. infula established based on LAMP in this study is accurate, rapid, sensitive, and low-cost, which is required for clinical treatment or forensic analysis when mushroom poisoning occurs.

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Extensive screening of cyclopeptide toxins in mushrooms by ultra-high-performance liquid chromatography coupled with quadrupole-Orbitrap mass spectrometry

Cited by 13 publications

References 34 publications

Proposing anti‐counterfeiting pharmacopoeia quality markers for Shenlingbaizhu granule based on UHPLC‐Q‐orbitrap‐MS identification

Proposing anti‐counterfeiting pharmacopoeia quality markers for Shenlingbaizhu granule based on UHPLC‐Q‐orbitrap‐MS identification

Simultaneous Determination of Multi-Class Mushroom Toxins in Mushroom and Biological Liquid Samples Using LC-MS/MS

Identification of Gyromitra infula: A Rapid and Visual Method Based on Loop-Mediated Isothermal Amplification

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