Extensive tissue damage of bovine ovaries after bipolar ovarian drilling compared to monopolar electrocoagulation or carbon dioxide laser

Hendriks, M.L.; Valk, Paul van der; Lambalk, Cornelis B.; Broeckaert, Mark A. M.; Homburg, Roy; Hompes, Peter G.A.

doi:10.1016/j.fertnstert.2008.10.046

Cited by 30 publications

(21 citation statements)

References 40 publications

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“…However, many factors, such as the energy modality, amount of energy applied (W × time × puncture), and depth of the puncture have been demonstrated to have an impact on the destructed ovarian volume [27] . Consequently, conclusive results about the relation between these parameters have not been obtained.…”

Section: Discussionmentioning

confidence: 99%

The Impact of Gradually Increasing Energy Dose on AMH Levels in Ovarian Drilling

Gürsoy

Kiseli²,

Pabuçcu³

et al. 2016

Gynecol Obstet Invest

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Background: To document alterations in Anti-Müllerian hormone (AMH) levels with different energy doses by monopolar cautery in ovarian drilling (OD) in rats. Methods: Forty Wistar rats were randomly divided into 4 groups that each contained 10 animals. Group A was the Sham group. Groups B, C and D were intervention groups to which OD was applied by monopolar cautery, with 20, 60 and 120 J/ovary, respectively. The total amount of energy was calculated using the following formula: energy (J) = power (W) × duration (s) × number of punctures. Results: The pre- and postoperative AMH levels for Groups A, B and C were not statistically and significantly different according to the Bonferroni correction (p > 0.0125). However, the postoperative AMH levels were significantly lower than the preoperative AMH levels in Group D (1.25 ng/ml (1.07-2.05) vs. 0.40 ng/ml (0.30-0.72); p = 0.012). Conclusions: AMH is a promising marker that can be used for the individualization of the energy dose to increase OD success, but the relationship between the energy and AMH has not been documented yet. This study demonstrates that postoperative changes in AMH levels could be observed only after a certain amount of energy was applied. Further experimental animal models are needed to conclusively address this issue.

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Section: Discussionmentioning

confidence: 99%

The Impact of Gradually Increasing Energy Dose on AMH Levels in Ovarian Drilling

Gürsoy

Kiseli²,

Pabuçcu³

et al. 2016

Gynecol Obstet Invest

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“…Cows are a suitable experimental model due to the similarities between their ovary size and location in the vaginal vault and estrous cycles and those of human females [18,21]. All animals subjected to the procedure showed typical macroscopic and microscopic lesions due to monopolar ovarian cauterization, with no lesions found in the needle path.…”

Section: Discussionmentioning

confidence: 99%

“…Slides were reviewed, the largest lesion in each ovary was quantified by ImageJ, and lesion area was estimated as part of a cylinder or a sphere. To calculate the first approximation, the lesion was estimated to have a uniform thickness of 3 mm because, as the needle has a diameter of 1 mm, we estimated 100% lesion extension for each side with a cylinder-shaped injury, as described elsewhere [18]. To calculate the second approximation, the lesion was estimated to have the volume of a sphere (V = 4/3*π*r 3 ) whose radius is equal to the square root of the area calculated in ImageJ over π (r = √A/π).…”

Section: Methodsmentioning

confidence: 99%

Adequacy of ovarian diathermy under ultrasound control: an experimental model

et al. 2013

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BackgroundTo develop a minimally invasive ovarian cauterization technique under transvaginal ultrasound control and evaluate the safety and feasability of monopolar cauterization to cause ovarian injury using female cattle of reproductive age as an experimental model.MethodExperimental study in a university research center was performed. Eleven female bovines of reproductive age were submitted to monopolar transvaginal ovarian cauterization. The right ovary (RO) was punctured at four sites and 40 W was applied for 5 s at each point, resulting in a total of 800 J (Joules) of thermal energy. In the left ovary (LO), the procedure was similar, with the same time and 80 W, resulting in a thermal energy of 1600 J. Macroscopic and microscopic lesions were assessed.ResultsOf 22 ovaries punctured, 20 were cauterized and exhibited macroscopic and typical microscopic lesions. No lesions could be found in the needle path. The measures of the areas of microscopic electrocautery lesions calculated estimating a cylindrical volume showed a median of 1.12% in the right ovary and 1.65% in the left ovary. When the estimate was calculated by spherical shape, the medians were 1.77% in the right ovary and 3.06% in the left ovary. There was a statistically significant difference in these two estimates (sphere, p = 0.008; cylinder, p = 0.021).ConclusionThe experimental animal model described for transvaginal ultrasound-guided ovarian needle cauterization seems to be feasible. The ovaries were successfully cauterized without injuries in needle path and more energy resulted in significantly more thermal lesion. The safety and effectiveness of this technique, theoretically less invasive than current ovarian drilling methods, could be tested in anovulatory women with PCOS.

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“…I read with interest the article by Hendriks et al (1), and I would like to raise certain considerations. Because ovaries with polycystic ovary syndrome (PCOS) in humans have different endocrinologic and morphologic properties, the normal bovine ovary model for the evaluation of the amount of damage at different energy modalities seems inadequate.…”

mentioning

confidence: 99%

“…This observation suggests that the tissue destruction is generally created on the cystic component of the ovary. Hendriks et al (1) applied and demonstrated the procedure on the solid tissue component of normal bovine ovaries.…”

mentioning

confidence: 99%

Is there any difference among the most frequently used laparoscopic ovarian drilling techniques?

Apı

2009

Fertility and Sterility

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Extensive tissue damage of bovine ovaries after bipolar ovarian drilling compared to monopolar electrocoagulation or carbon dioxide laser

Cited by 30 publications

References 40 publications

The Impact of Gradually Increasing Energy Dose on AMH Levels in Ovarian Drilling

The Impact of Gradually Increasing Energy Dose on AMH Levels in Ovarian Drilling

Adequacy of ovarian diathermy under ultrasound control: an experimental model

Is there any difference among the most frequently used laparoscopic ovarian drilling techniques?

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