Extensively Drug-Resistant New Delhi Metallo-β-Lactamase–Encoding Bacteria in the Environment, Dhaka, Bangladesh, 2012

Toleman, Mark A.; Bugert, Joachim Jakob; Nizam, Syed Asif

doi:10.3201/eid2106.141578

Cited by 57 publications

(35 citation statements)

References 15 publications

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“…5). Recent reports have shown a greater association of the NDM-1 genotype with sequence type 101, which is consistent with our findings as we also observed three NDM-1-bearing E. coli isolates belonging to ST101 (56,57). We identified NDM-5 to be present in E. coli isolates belonging to ST405 and ST167.…”

Section: Discussionsupporting

confidence: 82%

Molecular Epidemiology and Genome Dynamics of New Delhi Metallo-β-Lactamase-Producing Extraintestinal Pathogenic Escherichia coli Strains from India

Ranjan

Shaik

Mondal

et al. 2016

Antimicrob Agents Chemother

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cThe global dissemination and increasing incidence of carbapenem-resistant, Gram-negative organisms have resulted in acute public health concerns. Here, we present a retrospective multicenter study on molecular characterization of metallo-␤-lactamase (MBL)-producing clinical Escherichia coli isolates recovered from extraintestinal infections in two hospitals in Pune, India. We screened a large sample size of 510 E. coli isolates for MBL production wherein we profiled their molecular determinants, antimicrobial resistance phenotypes, functional virulence properties, genomic features, and transmission dynamics. Approximately 8% of these isolates were MBL producers, the majority of which were of the NDM-1 (69%) type, followed by NDM-5 (19%), NDM-4 (5.5%), and NDM-7 (5.5%). MBL producers were resistant to all antibiotics tested except for colistin, fosfomycin, and chloramphenicol, which were effective to various extents. Plasmids were found to be an effective means of dissemination of NDM genes and other resistance traits. All MBL producers adhered to and invaded bladder epithelial (T24) cells and demonstrated significant serum resistance. Genomic analysis of MBL-producing E. coli isolates revealed higher resistance but a moderate virulence gene repertoire. A subset of NDM-1-positive E. coli isolates was identified as dominant sequence type 101 (ST101) while two strains belonging to ST167 and ST405 harbored NDM-5. A majority of MBL-producing E. coli strains revealed unique genotypes, suggesting that they were clonally unrelated. Overall, the coexistence of virulence and carbapenem resistance in clinical E. coli isolates is of serious concern. Moreover, the emergence of NDM-1 among the globally dominant E. coli ST101 isolates warrants stringent surveillance and control measures.

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Section: Discussionsupporting

confidence: 82%

Molecular Epidemiology and Genome Dynamics of New Delhi Metallo-β-Lactamase-Producing Extraintestinal Pathogenic Escherichia coli Strains from India

Ranjan

Shaik

Mondal

et al. 2016

Antimicrob Agents Chemother

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“…bla CTX-M genes seem to be commonly detected from the environment in East Asia, Europe, and Australia (32)(33)(34)(35). bla NDM genes have been detected in the environment in Ban- gladesh and China (31,36). Novovic et al (37) could not detect bla NDM -positive bacteria from environmental waters in Serbia, which is recognized to be an area where NDM-1-producing bacteria are the most prevalent.…”

Section: Discussionmentioning

confidence: 99%

“…The difference in ␤-lactamase gene prevalence may be due to the difference in the methodology of selection of E. coli isolates. bla CTX-M-15 is also the most prevalent ␤-lactamase gene conferring ESC resistance among bacteria in the environment in Bangladesh (31). bla CTX-M genes seem to be commonly detected from the environment in East Asia, Europe, and Australia (32)(33)(34)(35).…”

Section: Discussionmentioning

confidence: 99%

Distribution and Relationships of Antimicrobial Resistance Determinants among Extended-Spectrum-Cephalosporin-Resistant or Carbapenem-Resistant Escherichia coli Isolates from Rivers and Sewage Treatment Plants in India

Akiba

Sekizuka

Yamashita

et al. 2016

Antimicrob Agents Chemother

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kTo determine the distribution and relationship of antimicrobial resistance determinants among extended-spectrum-cephalosporin (ESC)-resistant or carbapenem-resistant Escherichia coli isolates from the aquatic environment in India, water samples were collected from rivers or sewage treatment plants in five Indian states. A total of 446 E. coli isolates were randomly obtained. Resistance to ESC and/or carbapenem was observed in 169 (37.9%) E. coli isolates, which were further analyzed. These isolates showed resistance to numerous antimicrobials; more than half of the isolates exhibited resistance to eight or more antimicrobials. The bla NDM gene was detected in 14/21 carbapenem-resistant E. coli isolates: bla NDM-1 in 2 isolates, bla NDM-5 in 7 isolates, and bla NDM-7 in 5 isolates. The bla CTX-M gene was detected in 112 isolates (66.3%): bla CTX-M-15 in 108 isolates and bla CTX-M-55 in 4 isolates. We extracted 49 plasmids from selected isolates, and their whole-genome sequences were determined. Fifty resistance genes were detected, and 11 different combinations of replicon types were observed among the 49 plasmids. The network analysis results suggested that the plasmids sharing replicon types tended to form a community, which is based on the predicted gene similarity among the plasmids. Four communities each containing from 4 to 17 plasmids were observed. Three of the four communities contained plasmids detected in different Indian states, suggesting that the interstate dissemination of ancestor plasmids has already occurred. Comparison of the DNA sequences of the bla NDM -positive plasmids detected in this study with known sequences of related plasmids suggested that various mutation events facilitated the evolution of the plasmids and that plasmids with similar genetic backgrounds have widely disseminated in India.

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“…If no control measures are taken, in a few years, this same scenario can be observed for other genes such as blaNDM-1 (New Delhi metallo-β-lactamase), blaVIM and blaKPC (carbapenemases), or vanA (vancomycin resistance operon gene), and then it may be too late to act. Indeed, in some Asiatic regions, the dissemination of the gene blaNDM-1 through water, in rivers and drinking water supplies, is reported for several years, and this is mainly attributed to poor sanitation practices (Walsh et al 2011;Toleman et al 2015).…”

Section: Important Environmental Reservoirs Of Contaminant Antibioticmentioning

confidence: 99%

Antibiotic resistance in urban aquatic environments: can it be controlled?

Manaia

Macedo

Fatta‐Kassinos

et al. 2015

Appl Microbiol Biotechnol

185

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Over the last decade, numerous evidences have contributed to establish a link between the natural and human-impacted environments and the growing public health threat that is the antimicrobial resistance. In the environment, in particular in areas subjected to strong anthropogenic pressures, water plays a major role on the transformation and transport of contaminants including antibiotic residues, antibiotic-resistant bacteria, and antibiotic resistance genes. Therefore, the urban water cycle, comprising water abstraction, disinfection, and distribution for human consumption, and the collection, treatment, and delivery of wastewater to the environment, is a particularly interesting loop to track the fate of antibiotic resistance in the environment and to assess the risks of its trans-mission back to humans. In this article, the relevance of different transepts of the urban water cycle on the potential enrichment and spread of antibiotic resistance is reviewed. According to this analysis, some gaps of knowledge, research needs, and control measures are suggested. The critical rationale behind the measures suggested and the desirable involvement of some key action players is also discussed.

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Extensively Drug-Resistant New Delhi Metallo-β-Lactamase–Encoding Bacteria in the Environment, Dhaka, Bangladesh, 2012

Cited by 57 publications

References 15 publications

Molecular Epidemiology and Genome Dynamics of New Delhi Metallo-β-Lactamase-Producing Extraintestinal Pathogenic Escherichia coli Strains from India

Molecular Epidemiology and Genome Dynamics of New Delhi Metallo-β-Lactamase-Producing Extraintestinal Pathogenic Escherichia coli Strains from India

Distribution and Relationships of Antimicrobial Resistance Determinants among Extended-Spectrum-Cephalosporin-Resistant or Carbapenem-Resistant Escherichia coli Isolates from Rivers and Sewage Treatment Plants in India

Antibiotic resistance in urban aquatic environments: can it be controlled?

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