External Inosculation as a Feature of Revascularization Occurs After Free Transplantation of Murine Liver Grafts

Kuehl, A.-R.; Abshagen, Kerstin; Eipel, Christian; Laschke, Matthias W.; Menger, Michael D.; Laue, Michael; Vollmar, Brigitte

doi:10.1111/j.1600-6143.2012.04336.x

Cited by 3 publications

(1 citation statement)

References 68 publications

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“…Therefore, newer approaches for faster vascularization are required. The rapid, external inosculation of preformed vascular networks with host vessels may ensure adequate angiogenesis and survival of the implanted cells [133], which is thought to be promoted by cultivating prevascularized tissue constructs in an angiogenic ECM [134].…”

Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

State-of-the-Art Strategies for the Vascularization of Three-Dimensional Engineered Organs

Min

Yoo

2019

VSI

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Engineering three-dimensional (3D) implantable tissue constructs is a promising strategy for replacing damaged or diseased tissues and organs with functional replacements. However, the efficient vascularization of new 3D organs is a major scientific and technical challenge since large tissue constructs or organs require a constant blood supply to survive in vivo. Current approaches to solving this problem generally fall into the following three major categories: (a) cell-based, (b) angiogenic factor-based, and (c) scaffold-based. In this review, we summarize state-of-the-art technologies that are used to develop complex, stable, and functional vasculature for engineered 3D tissue constructs and organs; additionally, we have suggested directions for future research.

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Section: Conclusion and Future Perspectivesmentioning

confidence: 99%

State-of-the-Art Strategies for the Vascularization of Three-Dimensional Engineered Organs

Min

Yoo

2019

VSI

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In vitro dynamic perfusion of prevascularized OECs-DBMs (outgrowth endothelial progenitor cell - demineralized bone matrix) complex fused to recipient vessels in an internal inosculation manner

Chen

Cai

Shi³

et al. 2022

Bioengineered

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The current research on seed cells and scaffold materials of bone tissue engineering has achieved milestones. Nevertheless, necrosis of seed cells in center of bone scaffold is a bottleneck in tissue engineering. Therefore, this study aimed to investigate the in vivo inosculation mechanism of recipient microvasculature and prevascularized outgrowth endothelial progenitor cells (OECs)-demineralized bone matrix (DBM) complex. A dorsal skinfold window-chamber model with tail vein injection of Texas red-dextran was established to confirm the optimal observation time of microvessels. OECs-DBM complex under static and dynamic perfusion culture was implanted into the model to analyze vascularization. OECs-DBM complex was harvested on 12th day for HE staining and fluorescent imaging. The model was successfully constructed, and the most appropriate time to observe microvessels was 15 min after injection. The ingrowth of recipient microvessels arcoss the border of OECs-DBM complex increased with time in both groups, and more microvessels across the border were observed in dynamic perfusion group on 3rd, 5th, 7th day. Fluorescent integrated density of border in dynamic perfusion group was higher at all-time points, and the difference was more significant in central area. Fluorescent imaging of OECs-DBM complex exhibited that no enhanced green fluorescent protein-positive cells were found beyond the verge of DBM scaffold in both groups. In vitro prevascularization by dynamic perfusion culture can increase and accelerate the blood perfusion of OECs-DBM complex obtained from recipient microvasculature by internal inosculation. Accordingly, this approach may markedly contribute to the future success of tissue engineering applications in clinical practice.

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Generation of co-culture spheroids as vascularisation units for bone tissue engineering

Walser

Metzger²,

Görg³

et al. 2013

eCM

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Cell spheroids represent attractive building units for bone tissue engineering, because they provide a threedimensional environment with intensive direct cellcell contacts. Moreover, they allow for co-culture of both osteoblasts and vessel-forming cells, which may markedly increase their survival and vascularisation after transplantation. To test this hypothesis, we generated coculture spheroids by aggregating different combinations of primary human osteoblasts (HOB), human dermal microvascular endothelial cells (HDMEC) and normal human dermal fibroblasts (NHDF) using the liquid overlay technique. Mono-culture spheroids consisting either of HOB or HDMEC served as controls. After in vitro characterisation, the different spheroids were transplanted into dorsal skinfold chambers of CD1 nu/nu mice to study in vivo their viability and vascularisation over a 2-week observation period by means of repetitive intravital fluorescence microscopy and immunohistochemistry. In vitro, co-culture spheroids containing HDMEC rapidly formed dense tubular vessel-like networks within 72 h and exhibited a significantly decreased rate of apoptotic cell death when compared to mono-culture HDMEC spheroids. After transplantation, these networks interconnected to the host microvasculature by external inosculation. Of interest, this process was most pronounced in HOB-HDMEC spheroids and could not further be improved by the addition of NHDF. Accordingly, HOB-HDMEC spheroids were larger when compared to the other spheroid types. These findings indicate that HOB-HDMEC spheroids exhibit excellent properties to preserve viability and to promote proliferation and vascularisation. Therefore, they may be used as functional vascularisation units in bone tissue engineering for the seeding of scaffolds or for the vitalisation of non-healing large bone defects.

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External Inosculation as a Feature of Revascularization Occurs After Free Transplantation of Murine Liver Grafts

Cited by 3 publications

References 68 publications

State-of-the-Art Strategies for the Vascularization of Three-Dimensional Engineered Organs

State-of-the-Art Strategies for the Vascularization of Three-Dimensional Engineered Organs

In vitro dynamic perfusion of prevascularized OECs-DBMs (outgrowth endothelial progenitor cell - demineralized bone matrix) complex fused to recipient vessels in an internal inosculation manner

Generation of co-culture spheroids as vascularisation units for bone tissue engineering

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