External Validation and Transferability of Nirs Models Developed for Detecting and Quantifying MBM in Intact Compound Feeding Stuffs

Abstract

The ban on the use of animal‐origin by‐products such as meat and bone meal (MBM) in compound feeds was one of the measures implemented in the European Union to stop the spread of bovine spongiform encephalopathy (BSE) and to prevent its reoccurrence. It is now clear that the blanket ban will only be lifted if reliable analytical methods are available that ensure the detection of animal by‐products. Near‐infrared spectroscopy (NIRS) is likely to be the most rapid method for testing feedingstuffs, enabling a substantial increase in the number of samples tested and providing instant detection of adulteration, especially when samples are analyzed in intact form. This study aimed to demonstrate the feasibility of NIRS predictive models for detecting and quantifying MBM in intact compound feedingstuffs, and to demonstrate the transferability of calibration models between two NIRS instruments. PRACTICAL APPLICATIONS This study shows the potential of near infrared spectroscopy (NIRS) as a fast screening method for detecting the adulteration of compound feedingstuffs with animal‐origin meals, useful for the feed industry and the inspection bodies. NIRS could provide the first line of defense of the food chain, allowing a large‐scale analysis and making more costly methods to be used more productively on suspect specimens.

Two-Dimensional Correlation Spectroscopy (2D-COS) Variable Selection for Near-Infrared Microscopy Discrimination of Meat and Bone Meal in Compound Feed

“…Due to the possibility of its causing a fatal neurodegenerative disease, bovine spongiform encephalopathy (BSE), 7,8 strict regulations have been enacted worldwide to prevent MBM from entering the feed chain of ruminant animals. 9,10 Nowadays, researchers have demonstrated the potential of optical methods, such as near-infrared (NIR) spectroscopy 11–13 and NIR microscopy, 14–18 to identify MBM in animal feed. Near-infrared microscopy shows the advantages of accuracy and reliability over NIR spectroscopy, 14–18 due to its unique analysis process, which focuses the NIR light onto an extremely small area of interest and usually scans hundreds, even thousands, of particles in one sample.…”