Extra domain A and type III connecting segment of fibronectin in assembly and cleavage

Ligation of the Fibrin-binding Domain by β-Strand Addition Is Sufficient for Expansion of Soluble Fibronectin

“…The present findings therefore support the existence of an allosteric network among the fibrin-binding domain [1][2][3][4][5] [12][13][14]. Such an open conformation may account for the unique ability of EDA-positive FN to be assembled into extensive fibrous networks by CHO cells (42). Interestingly, FN assembly associated with lymphatic valves in mice has been shown to require interaction of ␣9␤1 integrin with the ␣9␤1-recognition sequence in EDA rather than interaction of ␣5␤1 integrin with 10 FNIII (43).…”

EDA Fibronectin Isoform of Amniotic Fluid in Relation to Normal Pregnancy Stages and to Pregnancies Complicated by Fetal Postmaturity Syndrome

“…A clinical postmaturity syndrome of a fetus is thought to be a consequence of failing placental function [15] and FN containing alternatively spliced ED-A domain is one of active participants of fibrous network of placental extracellular matrices, [16] thus EDA-FN might be released from matrix to amniotic fluid during placental dysfunction.…”

IIICS de novo glycosylated fibronectin as a marker for invasiveness in urothelial carcinoma of the urinary bladder (UBC)

“…Isoforms of Fn are generated by alternative splicing of the ED-A, ED-B and IIICS region. The inclusion of the IIICS region provides additional proteolytic cleavage sites and modulates the role of the heparin II domain in Wbrillogenesis and cell spreading (Santas et al 2002;Abe et al 2005). Moreover, the IIICS domain is characterized by an alternative de novo O-linked glycosylation.…”