Extracción, purificación y transformación enzimática del ácido clorogénico da la pulpa de café

Durán, Luis Víctor Rodríguez

doi:10.24275/uami.6969z081q

Cited by 1 publication

(1 citation statement)

References 85 publications

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“…Por consiguiente en los estudios posteriores se usará esta fuente de nitrógeno. Por otra parte, la alcalinización del medio de cultivo, generalmente está asociado a la liberación de amoniaco como producto de la actividad proteolítica y la descomposición de los aminoácidos (Rodríguez, 2016).…”

Section: Efecto De Medios De Cultivo a Diferentes Concentraciones En unclassified

Producción de biosurfactantes lipopeptídicos por Bacillus subtilis en fermentación en medio sólido

Velasco¹,

Marcela²

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Biosurfactants are amphipathic molecules produced by microorganisms. They present the same applications that chemical surfactants. Although, they are less toxic and harmful for environment. Lipopeptides (LP) are one of the most efficient biosurfactants (BS). LP are produced mainly by bacillus species. However, they are not produced on a large scale because their production and purification is expensive and they present low productivity. Generally, BS are produced in submerged state fermentation with low productivity because of foaming, production of exopolysaccharides and low transfer oxygen affect the process. Solid state fermentation solves these problems. For this, solid state fermentation was used to produce LP from the surfactin by B. subtilis in order to improve the production and productivity. Firstly, two culture media were compared, one to production of biomass (nutrient broth) and other to production of LP (media reported by Yeh et al. (2016)). Both culture media were used in different concentrations. Media reported by Yeh et al. (2016) three times concentrated was selected because it was the most production of LP. Then, the presence of MnSO4 and nitrogen source (NH4NO3 y NaNO3) was evaluated in that media. The MnSO4 with the NH4NO3 in the media improved the production of LP. Later, the concentrations of the nutrients such as Mg 2+ , K + , Fe 2+ and Mn 2+ were evaluated in order to improve the production and the productivity of LP. The production did not improve but the productivity increased by 50%. A comparison was discovered

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Section: Efecto De Medios De Cultivo a Diferentes Concentraciones En unclassified

Producción de biosurfactantes lipopeptídicos por Bacillus subtilis en fermentación en medio sólido

Velasco¹,

Marcela²

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show abstract

Extracción, purificación y transformación enzimática del ácido clorogénico da la pulpa de café

Cited by 1 publication

References 85 publications

Producción de biosurfactantes lipopeptídicos por Bacillus subtilis en fermentación en medio sólido

Producción de biosurfactantes lipopeptídicos por Bacillus subtilis en fermentación en medio sólido

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