Extracellular and intracellular zilpaterol and clenbuterol quantification in Hep G2 liver cells by UPLC-PDA and UPLC–MS/MS

Piña-Olmos, Sofia; Dolores-Hernández, Mariana; Villaseñor, Alma; Díaz-Torres, Roberto; Bribiesca, Efrén Ramírez; López‐Arellano, Raquel; Ramírez-Noguera, Patricia

doi:10.1016/j.jpba.2020.113817

Cited by 7 publications

(4 citation statements)

References 30 publications

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“…To the best of our knowledge, clenbuterol hydrochloride (CLB) is usually used as a kind of β-receptor agonist drug. However, on account of its function for reducing muscle fat and increasing muscle protein content, sometimes CLB is added to the feed livestock of undesirable breeders by unscrupulous traders, making it a well-known “lean meat powder.” , …”

Section: Resultsmentioning

confidence: 99%

“…However, on account of its function for reducing muscle fat and increasing muscle protein content, sometimes CLB is added to the feed livestock of undesirable breeders by unscrupulous traders, making it a wellknown "lean meat powder." 48,49 In view of the high stability and excellent luminescence properties of compound 1, luminescence detection explorations toward CLB have been performed. Then, sample 1 and CLB solid were ground adequately and were dispersed in deionized water into a 1 mg/mL suspension separately.…”

Section: ■ Experimental Sectionmentioning

confidence: 99%

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Ultrasensitive Eu-Based MOF Luminescence Sensor for Clenbuterol Visible Recognition

Zhang,

Ding,

Jiao

et al. 2024

Inorg. Chem.

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Clenbuterol (CLB) as an illegal feed additive may cause a great security risk to food safety. However, convenient and efficient detection means for CLB in practical application remain a formidable challenge. Herein, a stable Eu-based organic framework {[H2N(CH3)2]2[Eu2(ttca)2]·H2O}n (compound 1) (H4ttca = [1,1′:2′,1″-terphenyl]-4,4′,4″,5′-tetracarboxylic acid) has been harvested, exhibiting excellent chemical stability and thermal stability. Luminescence investigation reveals that compound 1 can sensitively and selectively detect CLB without being affected by different components from simulated serum and urine (limit detection: 22.7 nM). Furthermore, sensor 1 can also be applicable to CLB recognition in real swine feeds, presenting excellent anti-interference performance. The good cyclicity of compound 1 endows CLB determination with many advantages: low cost, high stability, and simplicity. Importantly, in view of the indication of the luminescence color (red to blue), test membranes were fabricated and employed for convenient and fast CLB detection, providing a valuable scheme for the visual monitoring of CLB in meat products. This work enriches rare earth metal compounds and luminescence sensor portfolios and breaks the concentration record (nM) for detecting CLB compared with reported complex materials, providing an effective monitoring platform for CLB visually.

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Section: Resultsmentioning

confidence: 99%

Section: ■ Experimental Sectionmentioning

confidence: 99%

Ultrasensitive Eu-Based MOF Luminescence Sensor for Clenbuterol Visible Recognition

Zhang,

Ding,

Jiao

et al. 2024

Inorg. Chem.

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“…The matrix effect commonly exists for the determination of analytes based on mass spectrometry. Even after sufficient sample preparation steps for extract cleanup, the matrix effect, which presents a major interference for β-agonist detection, cannot be completely eliminated. , The steps of removal or weakening of the matrix effect can increase the sensitivity, the stability, and the accuracy. After the thorough research on the sample preparation methods for the three experimented matrices, their matrix effect is evaluated to determine the result of cleanup and the way of quantification (internal or external calibration).…”

Section: Resultsmentioning

confidence: 99%

Determination of 18 β-Agonists in Aquatic Products Using High-Performance Liquid Chromatography Tandem Mass Spectrometry

Han

Chen

Yang

et al. 2022

ACS Food Sci. Technol.

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An effective and efficient method for the determination of 18 β-agonists in aquatic products using liquid chromatography tandem mass spectrometry (LC-MS/MS) was successfully established. The method optimization was conducted based on incurred samples to guarantee its practicability. In this method, the sample was hydrolyzed with β-glucuronidase-arylsulfatase in ammonium acetate solution and treated successively with protein precipitation in 5% trichloroacetic acid in water, lipid removal with n-hexane, and further concentration and cleanup by solid-phase extraction (hydrophilic–lipophilic balanced cartridge, primary–secondary amine cartridge). Isotope-labeled internal standards and matrix-matched calibration curves were used for quantification. The limit of quantification (LOQ) was verified at 0.5–1.0 μg/kg (S/N > 10). This method was evaluated with five different matrices, including eel, roasted eel, pufferfish, shrimp, and crab, at 1×, 2×, and 10× LOQs, with good recoveries (>77%) and high replicate repeatability (relative standard deviations (RSDs) <13%, intraday batch repeatability) and further validated by three other accredited laboratories. A total of 3 samples out of 37 tested aquatic products were found positive for unknown incur sources (ractopamine, 0.38–2.1 μg/kg; salbutamol 3.0 μg/kg; tulobuterol 2.9 μg/kg; clenbuterol 1.3 μg/kg; and clenpenterol, 0.3 μg/kg). The method has demonstrated to be reliable, sensitive, and stable for multiresidue screening of β-agonists for food safety monitoring.

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“…Currently, the detection techniques for LMP residues in animal feed and food include high-performance liquid chromatography (HPLC) [20,21], liquid chromatography-mass spectrometry (LC-MS) [22,23], gas chromatography-mass spectrometry (GC-MS) [24], ultrahigh-performance liquid chromatography (UHPLC) [25], and capillary electrophoresis (CE) [26], etc. Although the above methods have lower limits of detection and better stability, they have the disadvantages of long detection time, expensive instruments, and complicated operation.…”

Section: Introductionmentioning

confidence: 99%

Advance in Detection Technique of Lean Meat Powder Residues in Meat Using SERS: A Review

Guo,

Peng,

Qin

et al. 2023

Molecules

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Food that contains lean meat powder (LMP) can cause human health issues, such as nausea, headaches, and even death for consumers. Traditional methods for detecting LMP residues in meat are often time-consuming and complex and lack sensitivity. This article provides a review of the research progress on the use of surface–enhanced Raman spectroscopy (SERS) technology for detecting residues of LMP in meat. The review also discusses several applications of SERS technology for detecting residues of LMP in meat, including the enhanced detection of LMP residues in meat based on single metal nanoparticles, combining metal nanoparticles with adsorbent materials, combining metal nanoparticles with immunizing and other chemicals, and combining the SERS technology with related techniques. As SERS technology continues to develop and improve, it is expected to become an even more widely used and effective tool for detecting residues of LMP in meat.

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Extracellular and intracellular zilpaterol and clenbuterol quantification in Hep G2 liver cells by UPLC-PDA and UPLC–MS/MS

Cited by 7 publications

References 30 publications

Ultrasensitive Eu-Based MOF Luminescence Sensor for Clenbuterol Visible Recognition

Ultrasensitive Eu-Based MOF Luminescence Sensor for Clenbuterol Visible Recognition

Determination of 18 β-Agonists in Aquatic Products Using High-Performance Liquid Chromatography Tandem Mass Spectrometry

Advance in Detection Technique of Lean Meat Powder Residues in Meat Using SERS: A Review

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