1998
DOI: 10.1152/ajprenal.1998.275.6.f962
|View full text |Cite
|
Sign up to set email alerts
|

Extracellular ATP causes apoptosis and necrosis of cultured mesangial cells via P2Z/P2X7receptors

Abstract: Mesangial cells undergo cell death both by apoptosis and necrosis during glomerular disease. Since nucleotides are released from injured and destroyed cells in the glomerulus, we examined whether extracellular ATP and its receptors may regulate cell death of cultured mesangial cells. Addition of extracellular ATP (300 μM to 5 mM) to cultured rat mesangial cells for 90 min caused a 5.8-fold increase in DNA fragmentation (terminal deoxynucleotidyl transferase assay) and a 4.2-fold increase in protein levels of t… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

6
107
1
4

Year Published

2000
2000
2013
2013

Publication Types

Select...
9
1

Relationship

0
10

Authors

Journals

citations
Cited by 118 publications
(120 citation statements)
references
References 33 publications
6
107
1
4
Order By: Relevance
“…EIIaE and E2F are also members of the E protein family; both control cell cycle progression [67][68][69], and over-expression of E2F-1 can activate apoptosis [70]. The p53 tumor suppressor transcription factor controls expression of genes involved in the regulation of cell cycle progression and cell death [71], and there is an association between activation of the P2X 7 receptor and the p53 apoptotic pathway [72], and between increased expression of the P2X 7 receptor and p53 protein levels [73]. Additional studies are needed to determine whether the experimentally found four DNA-protein complexes correspond with any of the above suggested transcription factors.…”
Section: Discussionmentioning
confidence: 99%
“…EIIaE and E2F are also members of the E protein family; both control cell cycle progression [67][68][69], and over-expression of E2F-1 can activate apoptosis [70]. The p53 tumor suppressor transcription factor controls expression of genes involved in the regulation of cell cycle progression and cell death [71], and there is an association between activation of the P2X 7 receptor and the p53 apoptotic pathway [72], and between increased expression of the P2X 7 receptor and p53 protein levels [73]. Additional studies are needed to determine whether the experimentally found four DNA-protein complexes correspond with any of the above suggested transcription factors.…”
Section: Discussionmentioning
confidence: 99%
“…Another problem has been that although cell volume change is perhaps the most definitive means of classifying cell death as essentially apoptotic or necrotic, reports as to whether P2X 7 induces shrinkage or swelling have been mixed (5)(6)(7)(8)(9)(10)(11)18). Indeed, problematic to our hypothesis that cell shrinkage is required for PS translocation (3,35), P2X 7 -induced shrinkage has been reported to be a relatively late (30 min) phenotype (7) when compared with PS translocation, which commences within seconds (though PS translocation was not directly measured in that study).…”
Section: Discussionmentioning
confidence: 99%
“…ATP and UTP stimulate the mitogen-activated protein kinase (MAPK) cascade and the MAPK pathway to promote proliferation of rat renal mesangial cells [143,145,157,323,392]. Extracellular ATP causes apoptosis and necrosis of cultured mesangial cells via P2X7 receptors [326], although P2X4 receptors may also be involved in the apoptotic actions [347]. The generation of reactive oxygen species in rat mesangial cells may contribute to P2X7 receptor-induced apoptotic cell death [125].…”
Section: Mesangial Cellsmentioning
confidence: 99%