Extracellular Biosynthesis of Gold Nanoparticles by Metal Resistance Bacteria: <i>Streptomyces griseus</i>

Derakhshan, Fatemeh Khadivi; Dehnad, Alireza; Salouti, Mojtaba

doi:10.1080/15533174.2011.618484

Cited by 27 publications

(8 citation statements)

References 16 publications

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“…Kalabegishvili et al 29 reported that, the synthesis of AuNPs was caused by the addition of Streptomyces glaucus 71MD biomass to chloroauric acid and the maximum absorption peak indicating the biosynthesis of gold nanoparticles was observed at 530 nm. On the other hand, Khadivi Derakhshan et al 30 stated that UV visible absorption spectrum of AuNPs produced with Streptomyces griseus cells exhibited a prominent and broad peak at 540 nm.…”

Section: Resultsmentioning

confidence: 99%

Artificial neural network approach for prediction of AuNPs biosynthesis by Streptomyces flavolimosus, characterization, antitumor potency in-vitro and in-vivo against Ehrlich ascites carcinoma

El-Naggar,

Rabei,

Elmansy

et al. 2023

Sci Rep

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Gold nanoparticles (AuNPs) have emerged as promising and versatile nanoparticles for cancer therapy and are widely used in drug and gene delivery, biomedical imaging, diagnosis, and biosensors. The current study describes a biological-based strategy for AuNPs biosynthesis using the cell-free supernatant of Streptomyces flavolimosus. The biosynthesized AuNPs have an absorption peak at 530–535 nm. The TEM images indicate that AuNPs were spherical and ranged in size from 4 to 20 nm. The surface capping molecules of AuNPs are negatively charged, having a Zeta potential of − 10.9 mV. FTIR analysis revealed that the AuNPs surface composition contains a variety of functional groups as –OH, C–H, N–, C=O, NH3+, amine hydrochloride, amide group of proteins, C–C and C–N. The bioprocess variables affecting AuNPs biosynthesis were optimized by using the central composite design (CCD) in order to maximize the AuNPs biosynthesis. The maximum yield of AuNPs (866.29 µg AuNPs/mL) was obtained using temperature (35 °C), incubation period (4 days), HAuCl4 concentration (1000 µg/mL) and initial pH level 6. Comparison was made between the fitness of CCD versus Artificial neural network (ANN) approach based on their prediction and the corresponding experimental results. AuNPs biosynthesis values predicted by ANN exhibit a more reasonable agreement with the experimental result. The anticancer activities of AuNPs were assessed under both in vitro and in vivo conditions. The results revealed a significant inhibitory effect on the proliferation of the MCF-7 and Hela carcinoma cell lines treated with AuNPs with IC50 value of 13.4 ± 0.44 μg/mL and 13.8 ± 0.45 μg/mL for MCF-7 and Hela cells; respectively. Further, AuNPs showed potential inhibitory effect against tumor growth in tumor-bearing mice models. AuNPs significantly reduced the tumor volume, tumor weight, and decreased number of viable tumor cells in EAC bearing mice.

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Section: Resultsmentioning

confidence: 99%

Artificial neural network approach for prediction of AuNPs biosynthesis by Streptomyces flavolimosus, characterization, antitumor potency in-vitro and in-vivo against Ehrlich ascites carcinoma

El-Naggar,

Rabei,

Elmansy

et al. 2023

Sci Rep

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“…Several bacterial strains are versatile biocatalysts for biotransformation of heavy metals and other toxic compounds [1–8]. In this context, the biogenic synthesis of metallic NPs by microorganisms such as Pseudomonas , Shewanella and Streptomyces strains has been reported [9–15].…”

Section: Introductionmentioning

confidence: 99%

Synthesis of extracellular gold nanoparticles using Cupriavidus metallidurans CH34 cells

Montero-Silva

Durán

Seeger

2017

IET nanobiotechnol.

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In this study, spontaneous synthesis of a gold (Au) colloid using cells of Cupriavidus metallidurans CH34 is reported, and compared with results obtained using cells of the model bacterium Escherichia coli MG1655. To investigate the synthesis mechanism, bacterial biomass and secretomes from both strains were incubated with Au(III) ions. Only CH34 cells were capable of producing extracellular dispersions of Au nanoparticles (NPs). Transmission electron microscopy images showed that AuNPs morphology was dominated by triangular and decahedral nanostructures. Energy dispersive X-ray spectroscopy and Fourier transform infrared spectra showed the presence of sulphur and vibrations associated to proteins. Average AuNPs diameter was obtained by dynamic light-scattering measurements (DLS), NP tracking analysis measurements and analysis of electron microscopy images. Moreover, DLS measurements showed that the biogenic colloid was stable after exposure to ultrasound, high ionic strength and extreme pH conditions. The biogenic AuNPs produced by strain CH34 did not show antibacterial activity, in contrast to biogenic silver NPs. Comparative bioinformatic analysis of genomes from strain CH34 and strain MG1655 showed potential CH34 proteins that may be electron donors during reduction of Au(III) ions. On the basis of these results, a mechanism for the extracellular Au reduction by strain CH34 is proposed.

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“…Song et al 38 declared that the quantitative measurement of the GNPs concentration was recorded at 540 nm. Khadivi Derakhshan et al 39 stated that the UV visible absorption spectrum of GNPs synthesized using cells of Streptomyces griseus showed a strong and broad peak at 540 nm. The biosynthesis of GNPs using marine Streptomyces griseus was confirmed by the transformation of the yellow colour of gold cations in HAuCl 4 solution into the pink colour of the colloidal GNPs and the maximum absorption peak was observed at 534 nm 40 .…”

Section: Resultsmentioning

confidence: 99%

Process optimization for gold nanoparticles biosynthesis by Streptomyces albogriseolus using artificial neural network, characterization and antitumor activities

El-Naggar,

El-Sawah,

Elmansy

et al. 2024

Sci Rep

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Gold nanoparticles (GNPs) are highly promising in cancer therapy, wound healing, drug delivery, biosensing, and biomedical imaging. Furthermore, GNPs have anti-inflammatory, anti-angiogenic, antioxidants, anti-proliferative and anti-diabetic effects. The present study presents an eco-friendly approach for GNPs biosynthesis using the cell-free supernatant of Streptomyces albogriseolus as a reducing and stabilizing agent. The biosynthesized GNPs have a maximum absorption peak at 540 nm. The TEM images showed that GNPs ranged in size from 5.42 to 13.34 nm and had a spherical shape. GNPs have a negatively charged surface with a Zeta potential of − 24.8 mV. FTIR analysis identified several functional groups including C–H, –OH, C–N, amines and amide groups. The crystalline structure of GNPs was verified by X-ray diffraction and the well-defined and distinct diffraction rings observed by the selected area electron diffraction analysis. To optimize the biosynthesis of GNPs using the cell-free supernatant of S. albogriseolus, 30 experimental runs were conducted using central composite design (CCD). The artificial neural network (ANN) was employed to analyze, validate, and predict GNPs biosynthesis compared to CCD. The maximum experimental yield of GNPs (778.74 μg/mL) was obtained with a cell-free supernatant concentration of 70%, a HAuCl4 concentration of 800 μg/mL, an initial pH of 7, and a 96-h incubation time. The theoretically predicted yields of GNPs by CCD and ANN were 809.89 and 777.32 μg/mL, respectively, which indicates that ANN has stronger prediction potential compared to the CCD. The anticancer activity of GNPs was compared to that of doxorubicin (Dox) in vitro against the HeP-G2 human cancer cell line. The IC50 values of Dox and GNPs-based treatments were 7.26 ± 0.4 and 22.13 ± 1.3 µg/mL, respectively. Interestingly, treatments combining Dox and GNPs together showed an IC50 value of 3.52 ± 0.1 µg/mL, indicating that they targeted cancer cells more efficiently.

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Extracellular Biosynthesis of Gold Nanoparticles by Metal Resistance Bacteria: Streptomyces griseus

Cited by 27 publications

References 16 publications

Artificial neural network approach for prediction of AuNPs biosynthesis by Streptomyces flavolimosus, characterization, antitumor potency in-vitro and in-vivo against Ehrlich ascites carcinoma

Artificial neural network approach for prediction of AuNPs biosynthesis by Streptomyces flavolimosus, characterization, antitumor potency in-vitro and in-vivo against Ehrlich ascites carcinoma

Synthesis of extracellular gold nanoparticles using Cupriavidus metallidurans CH34 cells

Process optimization for gold nanoparticles biosynthesis by Streptomyces albogriseolus using artificial neural network, characterization and antitumor activities

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