Extracellular Calcium Levels Strongly Influence Neural Crest Cell Galvanotaxis

Nuccitelli, Richard; Smart, Tanya

doi:10.2307/1541662

Cited by 34 publications

(16 citation statements)

References 11 publications

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“…Moreover, recent molecular data implicated KCNQ1 potassium channels [154] and NaV sodium channels [12,155,156] in the regulation of migration and invasiveness of several stem-like cell types. These findings implicate the cell-autonomous property V mem in migration control, and complement the longknown ability of stem cells to migrate in physiologicalstrength electric gradients in their environment [157][158][159]. Thus, an investigation of voltage in the guidance of stem cell position during regeneration and morphostasis is a crucial area for future work.…”

Section: Migrationsupporting

confidence: 65%

Role of Membrane Potential in the Regulation of Cell Proliferation and Differentiation

Sundelacruz

Levin

Kaplan

2009

Stem Cell Rev and Rep

425

386

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Biophysical signaling, an integral regulator of long-term cell behavior in both excitable and non-excitable cell types, offers enormous potential for modulation of important cell functions. Of particular interest to current regenerative medicine efforts, we review several examples that support the functional role of transmembrane potential (V(mem)) in the regulation of proliferation and differentiation. Interestingly, distinct V(mem) controls are found in many cancer cell and precursor cell systems, which are known for their proliferative and differentiation capacities, respectively. Collectively, the data demonstrate that bioelectric properties can serve as markers for cell characterization and can control cell mitotic activity, cell cycle progression, and differentiation. The ability to control cell functions by modulating bioelectric properties such as V(mem) would be an invaluable tool for directing stem cell behavior toward therapeutic goals. Biophysical properties of stem cells have only recently begun to be studied and are thus in need of further characterization. Understanding the molecular and mechanistic basis of biophysical regulation will point the way toward novel ways to rationally direct cell functions, allowing us to capitalize upon the potential of biophysical signaling for regenerative medicine and tissue engineering.

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Section: Migrationsupporting

confidence: 65%

Role of Membrane Potential in the Regulation of Cell Proliferation and Differentiation

Sundelacruz

Levin

Kaplan

2009

Stem Cell Rev and Rep

425

386

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“…Mammalian corneal epithelial cells migrate to the cathode at an EF of 100 mV/mm (Zhao et al, 1996;Farboud et al, 2000). A similarly consistent picture has emerged for the response of migratory neural crest cells to applied EFs (Stump and Robinson, 1983;Cooper and Keller, 1984;Nuccitelli and Smart, 1989). It is clear that some cells possess mechanisms for sensing and responding directionally to EFs of 10 mV/mm or less.…”

Section: Discussionmentioning

confidence: 83%

Electric field effects on human spinal injury: Is there a basis in the in vitro studies?

Robinson

Cormie

2007

Developmental Neurobiology

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An important basis for the clinical application of small DC electric current to mammalian spinal injury is the responses of neurons in culture to applied electric fields. Our recent finding that zebrafish neurons were unresponsive to applied fields prompted us to critically examine previous results. We conclude that compelling evidence for neuronal guidance and directional stimulation of growth toward either the cathode or anode in an electric field exists only for cultured Xenopus neurons, and not for any mammalian neurons. No basis for the reported success in treating spinal injury exists in the in vitro studies, and considerable research will be required if the conditions of field application in mammalian spinal injury are to be optimized.

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“…However, the iplA-independent EF-induced [Ca 2+ ] i elevation is essential because removal of Ca 2+ from the extracellular medium completely prevents the electrotactic response. This may explain why most reports suggest a role for Ca 2+ signalling in electrotaxis, (Cooper and Schliwa, 1985;Onuma and Hui, 1985;Nuccitelli and Smart, 1989;Nuccitelli and Smart, 1991;Fang et al, 1998;Trollinger et al, 2002;Mycielska and Djamgoz, 2004), whereas one report reports no [Ca 2+ ] i change (Brown and Loew, 1994). This discrepancy may be accounted for by the requirement for different levels of [Ca 2+ ] i rise that are required for the electrotactic response in different cell types.…”

Section: Discussionmentioning

confidence: 99%

“…However this suggestion has been controversial. For example, Ca 2+ dependence of electrotaxis has been observed in neural crest cells, embryo mouse fibroblasts, and fish and human keratocytes (Cooper and Schliwa, 1985;Onuma and Hui, 1985;Onuma and Hui, 1988;Nuccitelli and Smart, 1989;Nuccitelli and Smart, 1991;Fang et al, 1998;Trollinger et al, 2002). By contrast, two strains of mouse fibroblasts exhibit electrotaxis independent of Ca 2+ signalling (Brown and Loew, 1994).…”

Section: Introductionmentioning

confidence: 99%

Influx of extracellular Ca2+ is necessary for electrotaxis inDictyostelium

Shanley

Walczysko

Bain

et al. 2006

Journal of Cell Science

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Intracellular free Ca2+ ([Ca2+](i)) is a pivotal signalling element in cell migration and is thought to be required for chemotaxis of Dictyostelium. Ca2+ signalling may also be important for electrotaxis. However this suggestion has been controversial. We show that electric fields direct Dictyostelium cells to migrate cathodally and increase [Ca2+] i in Dictyostelium cells, as determined by Fluo-3 AM imaging and Ca-45(2+) uptake. Omission of extracellular Ca2+([Ca2+](e)) and incubation with EGTA abolished the electric-field-stimulated [Ca2+](i) rise and directional cell migration. This suggests a requirement for [Ca2+](e) in the electrotactic response. Deletion of iplA, a gene responsible for chemoattractant-induced [ Ca2+](i) increase, had only a minor effect on the electric-field-induced [Ca2+](i) rise. Moreover, iplA-null Dictyostelium cells showed the same electrotactic response as wild-type cells. Therefore, iplA-independent Ca2+ influx is necessary for electrotactic cell migration. These results suggest that the [ Ca2+](i) regulatory mechanisms induced by electric fields are different from those induced by cAMP and folic acid in Dictyostelium cells. Different roles of the iplA gene in chemoattractant-induced and electrically induced Ca2+ signalling, and different effects of [ Ca2+](i) elevation on chemotaxis and electrotaxis indicate that the chemoattractant and electric cues activate distinctive initial signalling elements

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Extracellular Calcium Levels Strongly Influence Neural Crest Cell Galvanotaxis

Cited by 34 publications

References 11 publications

Role of Membrane Potential in the Regulation of Cell Proliferation and Differentiation

Role of Membrane Potential in the Regulation of Cell Proliferation and Differentiation

Electric field effects on human spinal injury: Is there a basis in the in vitro studies?

Influx of extracellular Ca2+ is necessary for electrotaxis inDictyostelium

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