Extracellular collagenase isolated from
            <i>Streptomyces antibioticus</i>
            UFPEDA 3421: purification and biochemical characterization

Costa, Elizianne Pereira; Costa, Romero Marcos Pedrosa Brandão; Albuquerque, Wendell; Nascimento, Thiago Pajeú; Conniff, Amanda Sales; Cardoso, Kethylen Barbara Barbosa; Neves, Anna Gabrielly Duarte; Batista, Juanize Matias da Silva; Porto, Ana Lúcia Figueiredo

doi:10.1080/10826068.2023.2225090

Cited by 3 publications

(1 citation statement)

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“…In this context, polysaccharides from natural sources have garnered attention for their biocompatibility, biodegradability, and bioactive attributes. Cassia grandis, a tropical leguminous plant species, is recognized for harboring polysaccharides that exhibit medicinal promise [10,11]. Referred to as pink cassia, this plant is extensively utilized in traditional medicine and pharmaceutical pursuits [12].…”

Section: Introductionmentioning

confidence: 99%

Physicochemical Properties of a Bioactive Polysaccharide Film from Cassia grandis with Immobilized Collagenase from Streptomyces parvulus (DPUA/1573)

Ferreira,

Cardoso,

Brandão-Costa

et al. 2024

Cosmetics

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(1) Background: Polysaccharide films are promising vehicles for the delivery of bioactive agents such as collagenases, as they provide controlled release at the wound site, facilitating tissue regeneration. This study aimed to investigate the physicochemical properties of Cassia grandis polysaccharide films with immobilized collagenase from Streptomyces parvulus (DPUA/1573). (2) Methods: Galactomannan was extracted from Cassia grandis seeds for film production with 0.8% (w/v) galactomannan and 0.2% (v/v) glycerol with or without collagenases. The films underwent physical-chemical analyses: Fourier-transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), thermogravimetric analysis (TGA), color and opacity (luminosity-L*, green to red-a*, yellow to blue-b*, opacity-Y%), moisture content, water vapor permeability (WVP), thickness, contact angle, and mechanical properties. (3) Results: The results showed similar FTIR spectra to the literature, indicating carbonyl functional groups. Immobilizing bioactive compounds increased surface roughness observed in SEM. TGA indicated a better viability for films with immobilized S. parvulus enzymes. Both collagenase-containing and control films exhibited a bright-yellowish color with slight opacity (Y%). Mechanical tests revealed decreased rigidity in PCF (−25%) and SCF (−41%) and increased deformability in films with the immobilized bioactive compounds, PCF (234%) and SCF (295%). (4) Conclusions: Polysaccharide-based films are promising biomaterials for controlled composition, biocompatibility, biodegradability, and wound healing, with a potential in pharmacological applications.

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Section: Introductionmentioning

confidence: 99%