Extracellular cyclophilin A possesses chemotaxic activity in cattle

Takanashi, Satoru; Nochi, Tomonori; Abe, Miku; Itaya, Nanami; Urakawa, Megumi; Sato, Kei; Zhuang, Tao; Umemura, Saori; Hayashi, Tomohito; Kiku, Yoshio; Kitazawa, Haruki; Rose, Michael T.; Watanabe, Kouichi; Aso, Hisashi

doi:10.1186/s13567-015-0212-1

Cited by 12 publications

(14 citation statements)

References 25 publications

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“…Previous reports indicated the main functions of cyclophilins was protein folding and trafficking [16]. Cyclophilin A (CYPA), which has the capacity to bind the immunosuppressive drug cyclosporin A (CSA), was first purified from bovine thymocytes [17]. CYPA is thought to play critical roles in many cellular processes, such as cell transduction and immune modulation [18].…”

Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: CYPA promotes the progression and metastasis of serous ovarian cancer (SOC) in vitro and in vivo

Wang

Yue³

et al. 2019

J Ovarian Res

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Ovarian cancer (OC) is a type of gynaecological malignancy with high mortality in females. Serous ovarian cancer (SOC) is a distinct subtype of OC with poor early diagnosis. Given the limitations of traditional therapies, such as chemotherapy, targeted treatment is therefore a promising therapy to improve the survival rate of SOC patients. Cyclophilin A (CYPA) is a member of Cyclophilin family and thought to participates in multiple cellular processes such as cell transduction and immune modulation. Recently, various of studies indicated that CYPA has critical impact on cancer progression. CYPA could regulate cell proliferation, invasion, and chemoresistance of multiple types of cancers. However, it is still unclear whether it could affect ovarian cancer. In this study, we demonstrated that CYPA was highly expressed in SOC tissues compared with adjacent tissues. Further, CYPA was significantly associated with clinical stage and lymphnode metastasis of SOC patients. Additionally, data indicated that knockdown of CYPA by its shRNA dramatically reduces migration and invasion capacity of SOC cells in vitro and blocks tumor metastasis in vivo. Our study investigates the involvement of CYPA in the progression and metastasis of SOC, and therefore provides CYPA as a promising therapeutic target for SOC treatment.

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Section: Introductionmentioning

confidence: 99%

RETRACTED ARTICLE: CYPA promotes the progression and metastasis of serous ovarian cancer (SOC) in vitro and in vivo

Wang

Yue³

et al. 2019

J Ovarian Res

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“…Bacteria killed with 0.5% (w/v) of formalin were incubated for 30 min at 4 °C with 1000 μg/mL of either anti- S. aureus or control IgG, both of which were conjugated with FITC (Sigma-Aldrich), as described previously [ 25 ]. Untreated bacteria were prepared as a control.…”

Section: Methodsmentioning

confidence: 99%

Identification of a novel mechanism of action of bovine IgG antibodies specific for Staphylococcus aureus

Furukawa

Yoneyama

Hata

et al. 2018

Vet Res

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Staphylococcus aureus is a major pathogen that causes subclinical mastitis associated with huge economic losses to the dairy industry. A few vaccines for bovine mastitis are available, and they are expected to induce the production of S. aureus-specific antibodies that prevent bacterial adherence to host cells or promote opsonization by phagocytes. However, the efficacy of such vaccines are still under debate; therefore, further research focusing on improving the current vaccines by seeking additional mechanisms of action is required to reduce economic losses due to mastitis in the dairy industry. Here, we generated S. aureus-specific bovine IgG antibodies (anti-S. aureus) that directly inhibited bacterial growth in vitro. Inhibition depended on specificity for anti-S. aureus, not the interaction between Protein A and the fragment crystallizable region of the IgG antibodies or bacterial agglutination. An in vitro culture study using S. aureus strain JE2 and its deletion mutant JE2ΔSrtA, which lacks the gene encoding sortase A, revealed that the effect of anti-S. aureus was sortase-A-independent. Sortase A is involved in the synthesis of cell-wall-associated proteins. Thus, other surface molecules, such as membrane proteins, cell surface polysaccharides, or both, may trigger the inhibition of bacterial growth by anti-S. aureus. Together, our findings contribute insights into developing new strategies to further improve the available mastitis vaccine by designing a novel antigen on the surface of S. aureus to induce inhibitory signals that prevent bacterial growth.Electronic supplementary materialThe online version of this article (10.1186/s13567-018-0517-y) contains supplementary material, which is available to authorized users.

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“…Hydrocortisone resistant thymocytes (EL-4) secrete CypA that induces migration of the bone marrow cell precursors of neutrophils, T and B cells, and dendritic cells of mice to the periphery [50]. Recently, rbCypA was shown to attract bovine peripheral blood granulocytes, monocytes, and lymphocytes [51]. The rCypA from channel catfish and Chinese yellow catfish had chemotactic activity for leukocytes in vitro [52,53].…”

Section: Cypa-induced Leukocyte Chemotaxismentioning

confidence: 99%

“…The chemotactic activity of commercially available rCypA for monocytic THP-1 cells was prevented by CsA [58]. Substantial granulocyte migration toward rbCypA was inhibited by the addition of anti-bCypA antibody [51]. Leukocyte chemotaxis activity of CypA in the skin mucus of Chinese yellow catfish was also inhibited by the addition of anti-CypA antibodies [54].…”

Section: Inhibition Of the Chemotactic Activity Of Cypamentioning

confidence: 99%

Updates in understanding the role of cyclophilin A in leukocyte chemotaxis

Dawar

Zhao

et al. 2017

Journal of Leukocyte Biology

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Cyclophilin A (CypA), a well-recognized receptor for anti-inflammatory drug cyclosporine A (CsA) is a ubiquitous and multifunctional protein. Beside the diverse intracellular functions, CypA is secreted against inflammatory stimuli, where it activates and attracts leukocytes via CD147 to the stimulus site. Interestingly, it synergizes with other factors to induce leukocyte migration in different animals. However, the silencing and inhibition of CypA or CD147 inhibits leukocytes chemotaxis and inflammation. This review focuses on the advances made in understanding the mechanism of CypA-dependent leukocytes chemotaxis and hence, recognition of this factor as a possible therapeutic target in inflammatory diseases.

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Extracellular cyclophilin A possesses chemotaxic activity in cattle

Cited by 12 publications

References 25 publications

RETRACTED ARTICLE: CYPA promotes the progression and metastasis of serous ovarian cancer (SOC) in vitro and in vivo

RETRACTED ARTICLE: CYPA promotes the progression and metastasis of serous ovarian cancer (SOC) in vitro and in vivo

Identification of a novel mechanism of action of bovine IgG antibodies specific for Staphylococcus aureus

Updates in understanding the role of cyclophilin A in leukocyte chemotaxis

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