Extracellular Deoxyribonuclease Production by Yeasts

Cazin, John; Kozel, Thomas R.; Lupan, David M.; Burt, W. R.

doi:10.1128/jb.100.2.760-762.1969

Cited by 37 publications

(15 citation statements)

References 14 publications

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“…The differentiation of Serratia from Enterobacter species (9) relies in part on detection of the enzyme. Additionally, the ability to produce DNase is indicative of several yeast genera (2).…”

mentioning

confidence: 99%

Extracellular Deoxyribonuclease Production by Anaerobic Bacteria

Porschen¹,

Sonntag²

1974

Applied Microbiology

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The production of extracellular deoxyribonuclease was examined with anaerobic organisms isolated from clinical specimens. Nuclease activity was extraordinarily common. All strains of Fusobacterium, including eight species, as well as Bacteroides fragilis and B. melaninogenicus, displayed enzyme activity. Whereas the gram-positive bacteria were generally less productive, all strains of Clostridium perfringens, Peptostreptococcus intermedius, and P. anaerobius specifically produced deoxyribonuclease. The test is taxonomically valuable, particularly in the characterization of gram-positive cocci, since a deoxyribonucleaseproducing coccus indicates P. intermedius or P. anaerobius. Additionally, possession of the enzyme may prove to be a useful correlate of the potential pathogenicity of anaerobes.

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confidence: 99%

Extracellular Deoxyribonuclease Production by Anaerobic Bacteria

Porschen¹,

Sonntag²

1974

Applied Microbiology

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“…Our results support study who reported that both species produce extracellular DNase, but clinical isolates of C. neoformans produce significantly more extracellular DNase than environmental isolates 20 . Extracellular DNase activity exhibits an essential role of in the taxonomic and phylogenetic studies of the genus Cryptococcus 11 .…”

Section: Resultsmentioning

confidence: 99%

“…Then the inoculated plates were flooded with 1 N hydrochloric acid. Yeasts that depolymerized DNA contained in the medium exhibited clear zones surrounding the colonies, whereas those organisms which exhibited no extracellular DNase activity showed only the opaque background of precipitated DNA 20,11 .…”

Section: Determination Of Dnase Productionmentioning

confidence: 99%

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Extracellular Protease and DNase Activities in Clinical and Environmental Isolates of Cryptococcus neoformans Species Complex from Central India

Gumasta¹,

Nawange²,

Singh³

et al. 2019

J. Drug Delivery Ther.

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Enzymes are important not only for the growth and multiplication of the microorganism but also in the infection, penetration of the host tissue and encountering host defense mechanisms. This study aims to investigate extracellular protease and DNase activity in clinical (20) and 120 environmental isolates of C. neoformans species complex collected from different localities of central India. DNase test agar containing toluidine blue and Yeast Carbon Base (YCB) agar medium supplemented with 0.1% BSA + 0.01% polypeptone was employed for the screening of DNase and protease production respectively. DNase and protease production was detected by the appearance of clear zones around the colonies. On the basis of enzymatic activity and their Pz values, high protease production (Pz≤0.6) was observed by 14 (11.6 %) environmental and 4 (11.6 %) clinical strains on 5th day, whereas 35 (29.16 %) environmental and 8 (40 %) clinical strains were screened on 8th day of incubation. Similarly 13 (10.83%) environmental and 3 (15 %) clinical strains on the 5th day, however 32 (26.66%) environmental and 8 (40 %) clinical strains on the 8th day of incubation were found to be high DNase producing strains with low Pz value (Pz≤0.6). In the case of protease activity, no significant difference was observed whereas a significant difference has shown by clinical C. neoformans and C. gattii strains on the 5th day of DNase production (p < .001). Extracellular enzymes play a vital role in the pathogenicity and virulence of C. neoformans species complex, therefore, enzymes are considered as worthy targets for developing therapeutics. Keywords: Cryptococcus neoformans species complex, Extracellular enzymes, DNase, protease, virulence, Pz value

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“…In the environment, urease probably assists C. neoformans in converting urea into a usable nitrogen source in pigeon guano (Cox et al, 2000). DNAse production is found in bacterial pathogens (Sanchez and Colom, 2010), as well as non-pathogenic Cryptococcus, Rhodotorula and Tremella (Cazin et al, 1969). Interestingly, higher activity (and greater diversity) of DNAse was observed from clinical C. neoformans strains compared with environmental strains (Sanchez and Colom, 2010).…”

Section: Vertebrate Modelsmentioning

confidence: 99%

It's not all about us: evolution and maintenance ofCryptococcusvirulence requires selection outside the human host

Gerstein

Nielsen

2017

Yeast

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Cryptococcus is predominantly an AIDS-related pathogen that causes significant morbidity and mortality in immunocompromised patients. Research studies have historically focused on understanding how the organism causes human disease through the use of in vivo and in vitro model systems to identify virulence factors. Cryptococcus is not an obligate pathogen, however, as human-human transmission is either absent or rare. Selection in the environment must thus be invoked to shape the evolution of this taxa, and directly influences genotypic and trait diversity. Importantly, the evolution and maintenance of pathogenicity must also stem directly from environmental selection. To that end, here we examine abiotic and biotic stresses in the environment, and discuss how they could shape the factors that are commonly identified as important virulence traits. We identify a number of important unanswered questions about Cryptococcus diversity and evolution that are critical for understanding this deadly pathogen, and discuss how implementation of modern sampling and genomic tools could be utilized to answer these questions. Copyright © 2016 John Wiley & Sons, Ltd.

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Extracellular Deoxyribonuclease Production by Yeasts

Cited by 37 publications

References 14 publications

Extracellular Deoxyribonuclease Production by Anaerobic Bacteria

Extracellular Deoxyribonuclease Production by Anaerobic Bacteria

Extracellular Protease and DNase Activities in Clinical and Environmental Isolates of Cryptococcus neoformans Species Complex from Central India

It's not all about us: evolution and maintenance ofCryptococcusvirulence requires selection outside the human host

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