2014
DOI: 10.1007/s00424-014-1540-0
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Extracellular disulfide bridges stabilize TRPC5 dimerization, trafficking, and activity

Abstract: Crucial cysteine residues can be involved in the modulation of protein activity via the modification of thiol (-SH) groups. Among these reactions, disulfide bonds (S-S) play a key role in the folding, stability, and activity of membrane proteins. However, the regulation of extracellular cysteines in classical transient receptor potential (TRPC) channels remains controversial. Here, we examine the functional importance of the extracellular disulfide bond in TRPC5 in modulating channel gating and trafficking. Sp… Show more

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Cited by 26 publications
(24 citation statements)
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“…The I-V curve showed a typical doubly rectifying shape, consistent with previous reports (Fig. 3B) [2324252627]. The current at -60 mV increased from -10.…”
Section: Resultssupporting
confidence: 91%
“…The I-V curve showed a typical doubly rectifying shape, consistent with previous reports (Fig. 3B) [2324252627]. The current at -60 mV increased from -10.…”
Section: Resultssupporting
confidence: 91%
“…Ion channels are known to possess disulfide bonds to provide a structural scaffold or redox sensitivity. These include ion channels such as NMDA receptor, CLIC1, Kir2.2, cardiac ryanodine receptor, TWIK-1 channel, and TRPC5 (47)(48)(49)(50)(51)(52)(53). However, to our knowledge this is the first observation in which an ion channel uses the redox heterogeneity of a single specific Cys residue (Cys-258) to provide both oxidation sensitivity and protein stability.…”
Section: Discussionmentioning
confidence: 74%
“…2C and 4B). In general, making a dimer or tetramer construct takes a long time, and dimer or tetramer constructs usually do not work well due to the linker length or the flexibility of the linker (22). To combine the imaging methods and the FRET methods was useful for finding a membranetargeting domain or tetrameric assembly domain.…”
Section: Discussionmentioning
confidence: 99%
“…Collected proteins were then analyzed by Western blot. The experiment was performed as previously described in detail (22).…”
Section: Methodsmentioning
confidence: 99%