Extracellular DNA traps in a ctenophore demonstrate conserved immune cell behaviors in a non-bilaterian

Vandepas, Lauren E.; Stefani, Caroline; Traylor-Knowles, Nikki; Goetz, Frederick W.; Browne, William E.; Lacy‐Hulbert, Adam

doi:10.1101/2020.06.09.141010

Cited by 2 publications

(3 citation statements)

References 79 publications

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“…Cytology: Fixing of cells for histology was done with fresh hemolymph, as described in detail previously (22). Briefly, 100 µL of the collected hemolymph was applied to poly-L-lysine slides (Electron Microscopy Sciences, Hatfield, PA, USA) and allowed to rest for ten minutes at room temperature on a flat surface.…”

Section: Methodsmentioning

confidence: 99%

“…Due to the recent identification of DN in Puget Sound basket cockles (C. nuttallii), found through histological examination during health screening of potential broodstock, we set out to determine whether the DN was a transmissible cancer, as seen in other bivalves. We first examined hemolymph samples from cockles collected from Agate Pass, Washington (Figure 1A), a site where DN had previously been observed in cockles through histological analysis (22). Hemocytes of non-neoplastic cockles are highly adherent to tissue culture dishes and show a variety of cell types, many with pseudopodia (Figure 1B).…”

Section: Disseminated Neoplasia In Cockles Is a Bivalve Transmissible...mentioning

confidence: 99%

“…Approximately 30 individuals from 11 different locations were sampled. We used both a slide-smear hemocytology method (22) to analyze cell morphology and the kspecific qPCR assay to screen DNA from the hemolymph and tissue of each cockle. We first analyzed samples from Semiahmoo, WA, as histological analysis of cell morphology suggested that 2 individuals (SM26 and SM6) at that location had advanced DN (Figure 2A-D).…”

Section: Survey Of Puget Sound Reveals Evidence Of Three Lineages Of ...mentioning

confidence: 99%

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Multiple lineages of transmissible neoplasia in the basket cockle (C. nuttallii) with repeated horizontal transfer of mitochondrial DNA

Yonemitsu,

Sevigny,

Vandepas

et al. 2023

Preprint

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Transmissible cancers are clonal lineages of neoplastic cells able to infect multiple hosts, spreading through the environment as an infectious disease. Transmissible cancers have been identified in Tasmanian devils, dogs, and bivalves. Several lineages of bivalve transmissible neoplasias (BTN) have been identified in multiple bivalve species. In 2019 in Puget Sound, disseminated neoplasia was observed in basket cockles (Clinocardium nuttallii), a species that is important to the culture and diet of the Suquamish Tribe as well as other tribes with traditional access to the species. To investigate disseminated neoplasia in cockles, a nuclear locus was amplified from a collection of cockles from Agate Pass, and DNA sequences showed evidence of a single cancer lineage, termed CnuBTN1, in three cockles. We used cytology and a qPCR assay targeting sequences found in BTNs to analyze collections of cockles from eleven locations from Puget Sound and the Washington coastline to screen for BTN and identify the extent of cancer spread. Three lineages of BTN in cockles were identified, with CnuBTN1 being the most prevalent and geographically widespread and the others only found in one location each. While multiple nuclear loci show all samples of CnuBTN1 come from a single lineage, the mitochondrial alleles in each cockle with CnuBTN1 are different from each other, suggesting mitochondrial genomes of this cancer have been replaced multiple times during its evolution through horizontal transmission. The identification and analysis of these BTNs are critical for broodstock selection, management practices, and repopulation of declining cockle populations, which will enable the continued cultural connection and dietary use of the cockles by the local Tribes.

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Section: Methodsmentioning

confidence: 99%

Section: Disseminated Neoplasia In Cockles Is a Bivalve Transmissible...mentioning

confidence: 99%

Section: Survey Of Puget Sound Reveals Evidence Of Three Lineages Of ...mentioning

confidence: 99%

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Multiple lineages of transmissible neoplasia in the basket cockle (C. nuttallii) with repeated horizontal transfer of mitochondrial DNA

Yonemitsu,

Sevigny,

Vandepas

et al. 2023

Preprint

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Isolation and Maintenance of In Vitro Cell Cultures from the Ctenophore Mnemiopsis leidyi

Dieter

Vandepas

Browne

2022

Methods in Molecular Biology

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The ability to isolate, monitor, and examine specific cells of interest enables targeted experimental manipulations that would otherwise be difficult to perform and interpret in the context of the whole organism. In vitro primary cell cultures derived from ctenophores thus serve as an important tool for understanding complex cellular and molecular interactions that take place both within and between various ctenophore cell types. Here we describe methods for reliably generating and maintaining primary cell cultures derived from the lobate ctenophore Mnemiopsis leidyi that can be used for a wide variety of experimental applications.

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Extracellular DNA traps in a ctenophore demonstrate conserved immune cell behaviors in a non-bilaterian

Cited by 2 publications

References 79 publications

Multiple lineages of transmissible neoplasia in the basket cockle (C. nuttallii) with repeated horizontal transfer of mitochondrial DNA

Multiple lineages of transmissible neoplasia in the basket cockle (C. nuttallii) with repeated horizontal transfer of mitochondrial DNA

Isolation and Maintenance of In Vitro Cell Cultures from the Ctenophore Mnemiopsis leidyi

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