Extracellular domain of human calcium sensing receptor immobilized to silica beads as biomaterial: A rapid chromatographic method for recognizing ligands from complex matrix ‘Shuangdan’

Ru, Xu; Cheng, Peixuan; Meng, Kaili; Li, Linkang; Jiao, Meizhi; Zhao, Xue; Jia, Ping; Zheng, Xiaohui; Xiao, Chaoni

doi:10.1016/j.jchromb.2022.123409

Cited by 5 publications

(1 citation statement)

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“…Concerning the effect of plant-derived components on CaSR, olive leaf extract, which contains several bioactive components including polyphenols, has been reported to regulate the localization of AQP2 via CaSR [34] . Recently, ligand searches for herbal drug components based on binding a nity for CaSR and docking simulations revealed that several avonoids, phenolic acids, and triterpenes act as ligands and inhibit calcium oxalate accumulation in the kidneys in an ethylene glycol-induced nephrolithiasis model [35][36][37] . GRS is a Kampo medicine consisting of ve crude drugs including the medicinal parts of plants and sclerotia, but the ingredient-derived component responsible for the effects observed in this study is unclear.…”

Section: Discussionmentioning

confidence: 99%

The diuretic effect of Goreisan involves the modulation of aquaporin 2 localization via the calcium-sensing receptor

Ogura,

Fujitsuka,

Nahata

et al. 2024

Preprint

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Aquaporin 2 (AQP2) contributes to water reabsorption and primitive urine concentration by migrating to the luminal surface of the collecting ducts in an anti-diuretic hormone-stimulated manner, and the signaling pathway involved in AQP2 localization is a target for diuretics. This study investigated whether AQP2 is involved in the diuretic effect and mechanisms of Goreisan (GRS), a traditional Japanese Kampo medicine used to treat symptoms such as edema in patients with decreased urination. GRS exerted diuretic effects on desmopressin (dDAVP)-induced decreases in urine output and inhibited phosphorylation of AQP2 at Ser269 in the renal tissues of mice. Furthermore, GRS inhibited the migration of phosphorylated AQP2 to the luminal side following forskolin (FSK) stimulation using a 3D culture model of the kidney collecting duct cell line mIMCD-3. GRS induced a transient increase in the intracellular calcium concentration via the calcium-sensing receptor (CaSR) and suppressed the FSK-stimulated increase in cAMP production. These results suggest that GRS regulates urine volume by modulating the localization of AQP2 via CaSR.

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Section: Discussionmentioning

confidence: 99%