Extracellular enzyme production by different species of Trichoderma fungus for lemon peel waste bioconversion

Creating a novel genetic diversity of Trichoderma afroharzianum by γ-radiation for xylanase-cellulase production

“…The electrophoretic pattern of the proteins and the molecular weight (Mw) were investigated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) with a 5% (stacking gel) and 12.5% (separating gel) polyacrylamide gel [ 25 ] at constant amperage of 25 mA, and then proteins were stained with Coomassie Brilliant Blue R-250. Estimation of the Mw of protein bands was performed using Gel-Pro Analyzer 6.0 densitometry software (Media Cybernetics, Inc.) [ 8 ]. The Mw range of the protein marker (Sinaclon Company, Iran) was 11–245 kDa (kDa) [ 22 , 25 ].…”

“…Filamentous fungi such as Penicillium [ 5 ], Aspergillus [ 6 ], Phanerochaete [ 7 ], and Trichoderma [ 8 ] are cell factories for the high production of different lignocellulose degrading enzymes, and almost greatest significant industrialized source of enzymes belong to this category of organisms. Also, different species of Trichoderma fungi produce diverse extracellular enzymes including endo-xylanases (XYN) I, II, III, and IV, β-1,4-xylosidases I, and II, exo–glucanases or cellobiohydrolases (CBH) I and II, endo-glucanases I, II, III, IV, and V and β-1,4-D-glucosidases I, and II (BGL, EC 3.2.1.21), β-mannase, β-mannosidase, α- l -arabinofuranosidase, α-galactosidase, Acetylene xylan esterases, laccases (benzendiol: oxygen oxidoreductases), polygalacturonase, pectin lyase, and pectin esterase, that randomly biodegrade the glycosidic bonds of plant cell wall carbohydrates by different mechanisms of action [ 9 ].…”

Research Progress on Lignocellulosic Biomass Degradation Catalyzed by Enzymatic Nanomaterials

“…Haritos et al [52] showed that in high-protein applications, endoglucanase-treated cellulose fibers showed an improvement in fibrosis, refined fibers increased by 20.4%, while exoglucanase mixtures showed an improvement of 6.8%. Askari et al [53] studied the production of NAS114 by different fungi (Trichoderma aureum NAS106, Trichoderma African NAS107, Trichoderma sugarcane NAS108, pleural Trichoderma NAS109, Trichoderma harzianum NAS110, and Trichoderma leesei T. lixii) under underwater fermentation conditions. It is proved that the synergistic effect of endoglucanase and cellulose hydrolase is the key to total cellulase activity.…”

Isolation and characterization of cell wall and extracellular polysaccharides from cultures of the mycoparasitic strainTirochoderma koningiopsis

“…Various industrial and agricultural sectors make use of species within this genus as highlighted by Tyśkiewicz et al [13]. These fungi serve as a rich source of many enzymes that are capable of degrading both cellulose polymers and chitin [14]. These include endo-xylanases, exo-glucanases, endo-glucanohydrolases, chitinases, and laccases [15].…”