Extracellular Enzymes Produced by Vibrio alginolyticus Isolated from Environments and Diseased Aquatic Animals

Bunpa, Supansa; Sermwittayawong, Natthawan; Vuddhakul, Varaporn

doi:10.1016/j.proche.2016.01.002

Cited by 26 publications

(18 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This was presumably due to the occurrence of lysis of red blood cells by V. alginolyticus so that the number decreased. According to Bunpa et al (2016), V. alginolyticus can secrete hemolysins, which can lyse red blood cells. However, the decline was still in the normal range.…”

Section: Resultsmentioning

confidence: 99%

CONTROL OF Vibrio alginolyticus INFECTION IN ASIAN SEA BASS Lates calcarifer USING AMBON BANANA PLANT POWDER Musa paradisiacal THROUGH THE FEED

Pattah¹,

Wahjuningrum

Yuhana

et al. 2021

Indonesian Aquaculture Journal

View full text Add to dashboard Cite

Asian sea bass is one of economically important commodities in aquaculture. However, its culture often challenged by vibriosis infection which resulted in lower production. The study aimed to evaluate the effectiveness of the ambon banana plant powder application in feed as an effort to control the disease caused by Vibrio alginolyticus infection. Asian sea bass with total length of 7.48 ± 0.45 cm. Five different treatments were applied in this experiment, i.e. positive control, negative control, prevention, curative, and antibiotic application. The challenge test is carried out on the 15th day. Challenge test was performed with intramuscularly injection of V. alginolyticus at the cell density of 106 CFU/fish. The results showed the amount of feed consumption after the challenge test was highest in the curative and antibiotic control. However, these results were not significantly different (P>0.05) among preventive and curative treatments but were significantly different from controls (P<0.05). Fish treated with supplemented feed showed the highest total erythrocytes, total leukocytes, hemoglobin, respiratory burst, and lysozyme activity compared to controls. IL-1b gene expression increased after the challenge test with the highest level of expression in the curative treatment. It can be concluded that the administration of ambon banana plant powder (3 g/100 g diet) could control of V. alginolyticus infection in Asian sea bass and resulted 70.00% survival rate on prevention and 83.33% on curative treatments.

show abstract

Section: Resultsmentioning

confidence: 99%

CONTROL OF Vibrio alginolyticus INFECTION IN ASIAN SEA BASS Lates calcarifer USING AMBON BANANA PLANT POWDER Musa paradisiacal THROUGH THE FEED

Pattah¹,

Wahjuningrum

Yuhana

et al. 2021

Indonesian Aquaculture Journal

View full text Add to dashboard Cite

show abstract

“…Vibrio spp. that have been associated with diseases in animals and human often possess virulence factors, which are not available or present in the environmental Vibrio [1]. However, since Vibrio possesses highly plastic genome, the probability of horizontal transfer of the virulence genes between pathogenic and environmental Vibrio is high.…”

Section: Introductionmentioning

confidence: 99%

Virulence-associated genes and antibiotic resistance patterns of Vibrio spp. isolated from cultured marine fishes in Malaysia

et al. 2019

View full text Add to dashboard Cite

Background Vibriosis is an important bacterial disease of cultured marine fishes worldwide. However, information on the virulence and antibiotic resistance of Vibrio spp. isolated from fish are scarce. This study investigates the distribution of virulence associated genes and antibiotic resistance patterns of Vibrio spp. isolated from cage-cultured marine fishes in Malaysia. Results A total of 63 Vibrio spp. isolated from 62 cultured marine fishes in various geographical regions in Peninsular Malaysia were analysed. Forty-two of the isolates (66.7%) were positive for all chiA , luxR and vhpA , the virulence genes produced by pathogenic V. harveyi . A total of 62 Vibrio isolates (98%) had tlh gene of V. parahaemolyticus , while flaC gene of V. anguillarum was detected in 43 of isolates (68%). Other virulence genes, including tdh , trh , hlyA and toxR vc were absent from any of the isolates. Multiple antibiotic resistance (MAR) was exhibited in all strains of Harveyi clade, particularly against ampicillin, penicillin, polypeptides, cephems and streptomycin. The MAR index ranged between 0.06 and 0.56, and 75% of the isolates have MAR index of higher than 0.20. Host species and geographical origin showed no correlation with the presence of virulence genes and the antibiotic resistance patterns of Vibrio spp. Conclusions The study indicates that majority of Vibrio spp. isolated from cultured marine fishes possess virulence genes, but were not associated with human pathogen. However, the antibiotics resistance is a real concern and warrants ongoing surveillance. These findings represent an updated knowledge on the risk of Vibrio spp. to human health, and also provides valuable insight on alternative approaches to combat vibriosis in cultured fish.

show abstract

“…The current study could detect β‐hemolysis in 47% of V. parahaemolyticus , 63% of V. alginolyticus , and 100% of V. fluvialis isolates. In previous studies, 100% of the fish and shrimp‐borne V. alginolyticus were α‐haemolytic (Bunpa, Sermwittayawong, & Vuddhakul, ), and 15.8% of the V. parahaemolyticus from oysters and mussels were β‐haemolytic (Rojas, Matté, Dropa, Silva, & Matté, ).…”

Section: Resultsmentioning

confidence: 96%

“…Parallel to our results, more than 60% of oyster‐borne V. parahaemolyticus isolates were reported as positive for gelatinase, caseinase, phospholipase, lipase, amylase, and DNase (Costa et al, ). In a recent study, fish and shrimp associated V. alginolyticus isolates were 100, 100, 82, 70, and 59% positive for gelatinase, phospholipase, caseinase, amylase, and lipase tests, respectively (Bunpa et al, ).…”

Section: Resultsmentioning

confidence: 99%

Comparative prevalence and characterization of Vibrio spp. isolated from live and frozen white‐leg shrimp (Litopenaeus vannamei) in Korean markets

Silva

Hossain

Dahanayake

et al. 2018

Journal of Food Safety

View full text Add to dashboard Cite

We evaluated the prevalence and characteristics of Vibrio spp. in frozen and live samples of white‐leg shrimp (Litopenaeus vannamei) sold in Korea. The mean total bacterial counts of frozen and live white‐leg shrimp meat were approximately 2 ×109 and 1 ×1010 CFU/g, respectively. Eight V. parahaemolyticus isolates were recovered from frozen samples, whereas a total of 40 strains including 30 V. parahaemolyticus, 8 V. alginolyticus, 1 V. harveyi, and 1 V. fluvialis were isolated from live samples. The majority (≥92%) of the Vibrio spp. isolates were positive for six extracellular enzymatic tests, while 50 and 29% of the isolates were positive for hemolysis and slime production, respectively. In virulence gene profile, all V. parahaemolyticus isolates were positive for toxR and tlh genes, but negative for tdh and trh. V. alginolyticus isolates harbored collagenase, toxR, tlh, and VPI genes in 10, 75, 86, and 63%, respectively. Besides, V. fluvialis isolate was positive for toxR, vfh, and hupO genes. Moreover, 100, 91, 50, 45, and 27% of the isolates were resistant to ampicillin, piperacillin, colistin, vancomycin, and amikacin, respectively. These results implied that white‐leg shrimp‐borne Vibrio spp. are indicative of a potential consumer health risk. Practical applications Vibrio is a well‐known group of seafoodborne pathogenic bacteria. Since white‐leg shrimp is the most preferred shrimp variety in Korea, we sought to evaluate the prevalence and characteristics of Vibrio spp. in both frozen and live white‐leg shrimp from several retail markets. A total of 48 isolates were presumptively identified by biochemical tests. Subsequently, the gyrB gene sequencing and the neighbor‐joining phylogenetic tree confirmed the prevalence of 4 and 1 Vibrio species in live and frozen samples, respectively. Prevalence of Vibrio in frozen samples was much lower than live samples. Putative virulence of the Vibrio spp. was examined by both pathogenicity tests and PCR gene detection. Majority of the isolates were positive for pathogenicity tests and a heterogeneity in virulence gene prevalence was detected among Vibrio spp. Antibiotic susceptibility was examined by disc diffusion test for 25 antibiotics belong to 13 classes. The Vibrio isolates were highly resistant to penicillin group, however, others had different susceptibilities. In addition, we examined the total bacterial count (TBC) of the white‐leg shrimp meat by aerobic plate count method to evaluate their total bacterial prevalence. The obtained TBCs were higher than the recommended value. With regards to the results, it is worth noting that the white‐leg shrimp in Korean markets are indicative of a potential public health risk.

show abstract

Extracellular Enzymes Produced by Vibrio alginolyticus Isolated from Environments and Diseased Aquatic Animals

Cited by 26 publications

References 13 publications

CONTROL OF Vibrio alginolyticus INFECTION IN ASIAN SEA BASS Lates calcarifer USING AMBON BANANA PLANT POWDER Musa paradisiacal THROUGH THE FEED

CONTROL OF Vibrio alginolyticus INFECTION IN ASIAN SEA BASS Lates calcarifer USING AMBON BANANA PLANT POWDER Musa paradisiacal THROUGH THE FEED

Virulence-associated genes and antibiotic resistance patterns of Vibrio spp. isolated from cultured marine fishes in Malaysia

Comparative prevalence and characterization of Vibrio spp. isolated from live and frozen white‐leg shrimp (Litopenaeus vannamei) in Korean markets

Contact Info

Product

Resources

About