Extracellular glutamate: on-line monitoring using microdialysis coupled to enzyme-amperometric analysis

Zilkha, E.; Obrenovitch, Tihomir P.; Koshy, A.; Kusakabe, Hitoshi; Bennetto, H. P.

doi:10.1016/0165-0270(94)00214-2

Cited by 102 publications

(61 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…14 A number of sensor types have been developed for the measurement of Glu based on a variety of metabolic enzymes. [15][16][17][18][19][20] Attention has focused mainly on the use of l-glutamate oxidase (GluOx) that has FAD as the redox centre and molecular oxygen as a co-substrate, 21 producing hydrogen peroxide that can be detected amperometrically 19,20,[22][23][24][25][26][27][28][29][30] or spectroscopically. 31,32 The oxidative deamination catalysed by GluOx 21 can be represented by the following steps: l-Glutamate + H 2 O + GluOx/FAD ?…”

mentioning

confidence: 99%

Biosensor for Neurotransmitter L-Glutamic Acid Designed for Efficient Use of L-Glutamate Oxidase and Effective Rejection of Interference

Ryan

Lowry²,

O’Neill³

1997

Analyst

124

179

View full text Add to dashboard Cite

An amperometric biosensor for l-glutamic acid (Glu) was constructed by the adsorption and dip coating of l-glutamate oxidase (GluOx, 200 U ml 21 phosphate buffer, pH 7.4) onto 60-mm radius Teflon-coated Pt wire (1 mm exposed length). The enzyme was then trapped on the surface by electropolymerisation of o-phenylenediamine that also served to block electroactive interference. This procedure afforded electrodes with similar substrate sensitivity compared with the classical approach of immobilising enzyme from a solution of monomer, and represents an approximately 10 000-fold increase in the yield of biosensors from a batch of enzyme. A number of strategies were examined to enhance the sensitivity and selectivity of the Pt/PPD/GluOx sensors operating at 0.7 V versus SCE. Pre-coating the Pt with lipid and incorporation of the protein bovine serum albumin into the polymer matrix were found to improve the performance of the electrode. The sensors had a fast response time, high sensitivity to Glu, with an LOD of about 0.3 mmol l 21 , and possessed selectivity characteristics suggesting that monitoring Glu in biological tissues in vivo may be feasible.

show abstract

mentioning

confidence: 99%

Biosensor for Neurotransmitter L-Glutamic Acid Designed for Efficient Use of L-Glutamate Oxidase and Effective Rejection of Interference

Ryan

Lowry²,

O’Neill³

1997

Analyst

124

179

View full text Add to dashboard Cite

show abstract

“…L-glutamate, the most common excitatory neurotransmitter in the mammalian central nervous system (CNS), primarily regulates presynaptic and postsynaptic receptors [1][2][3] . Abnormal transmission of glutamate can cause neurological diseases such as communication dysfunction, cognitive impairments, schizophrenia, Parkinson's disease, stroke and epilepsy 4,5 .…”

Section: Introductionmentioning

confidence: 99%

An implantable microelectrode array for simultaneous L-glutamate and electrophysiological recordings in vivo

et al. 2015

View full text Add to dashboard Cite

L-glutamate, the most common excitatory neurotransmitter in the mammalian central nervous system (CNS), is associated with a wide range of neurological diseases. Because neurons in CNS communicate with each other both electrically and chemically, dualmode (electric and chemical) analytical techniques with high spatiotemporal resolution are required to better understand glutamate function in vivo. In the present study, a silicon-based implantable microelectrode array (MEA) composed of both platinum electrochemical and electrophysiological microelectrodes was fabricated using micro-electromechanical system. In the MEA probe, the electrophysiological electrodes have a low impedance of 0.018 MΩ at 1 kHz, and the electrochemical electrodes show a sensitivity of 56 pA µM −1 to glutamate and have a detection limit of 0.5 µM. The MEA probe was used to monitor extracellular glutamate levels, spikes and local field potentials (LFPs) in the striatum of anaesthetised rats. To explore the potential of the MEA probe, the rats were administered to KCl via intraperitoneal injection. K + significantly increases extracellular glutamate levels, LFP low-beta range (12-18 Hz) power and spike firing rates with a similar temporal profile, indicating that the MEA probe is capable of detecting dual-mode neuronal signals. It was concluded that the MEA probe can help reveal mechanisms of neural physiology and pathology in vivo.

show abstract

“…12 With respect to the simplicity of use, the short analytical time and the informational advantages to be gained from the direct and continuous analytic performance, 13 these systems are superior to off-line methods, such as liquid chromatography or capillary electrophoresis, and have provided some information of great importance. 14,15 Actually, chemical sensors with the ability to continuously sense analytes have attracted considerable attention, and many applications have appeared in the literature. In the last two decades, many analytical methods have been reported for such measurements, including spectrophotometry, 16,17 fluorometry, 18,19 chemiluminescence, 20,21 amperometry, [22][23][24] and voltammetry.…”

mentioning

confidence: 99%

Electrochemical Determination of Minoxidil in Different Pharmaceutical Formulations by Flow Injection Analysis

Pfaffen

Ortiz

2006

ANAL. SCI.

View full text Add to dashboard Cite

Extracellular glutamate: on-line monitoring using microdialysis coupled to enzyme-amperometric analysis

Cited by 102 publications

References 25 publications

Biosensor for Neurotransmitter L-Glutamic Acid Designed for Efficient Use of L-Glutamate Oxidase and Effective Rejection of Interference

Biosensor for Neurotransmitter L-Glutamic Acid Designed for Efficient Use of L-Glutamate Oxidase and Effective Rejection of Interference

An implantable microelectrode array for simultaneous L-glutamate and electrophysiological recordings in vivo

Electrochemical Determination of Minoxidil in Different Pharmaceutical Formulations by Flow Injection Analysis

Contact Info

Product

Resources

About