Extracellular Hsp70 inhibits pro-inflammatory cytokine production by IL-10 driven down-regulation of C/EBPβ and C/EBPδ

Borges, Thiago J.; Lopes, Rafael Lisboa; Pinho, Nathana Gustavo; Machado, Felipe D.; Souza, Ana Paula Duarte de; Bonorino, Cristina

doi:10.3109/02656736.2013.798037

Cited by 46 publications

(29 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Glutamine ¼ glutamine, LPS ¼ endotoxin *p < 0.05, **p < 0.005. model of sepsis model using, either lipoteichoic acid or LPS, shows that pre-treatment of human neutrophils with recombinant HSP70 prior to the administration of endotoxin attenuates the release of TNF-a [41]. This is corroborated in other in vitro animal models where the use of recombinant HSP70 provides a similar antiinflammatory mode of action by inhibiting C/EBPb and C/EPBd transcription factors from signalling via TLR2-ERK-STAT2-1L10 pathway, attenuating the release of pro-inflammatory mediators [42]. Converse to this, when healthy subjects were inoculated with endotoxin, levels of HSP70 and proinflammatory mediators …”

Section: Discussionsupporting

confidence: 60%

The effect of 2 mMol glutamine supplementation on HSP70 and TNF-α release by LPS stimulated blood from healthy children

et al. 2015

View full text Add to dashboard Cite

s u m m a r yObjective: Glutamine has been shown to promote heat shock protein 70 (HSP70) release both within experimental in vitro models of sepsis (2e10 mM) and in adults post trauma (0.5 g/kg), although the efficacy varies and is dependent on the model used. The effect of glutamine supplementation on HSP70 release in children is less clear. Therefore, the aim of this study was to investigate the effect of 2 mM glutamine added to incubation media on HSP70 and inflammatory mediator release in an in vitro model of paediatric sepsis using whole blood from healthy paediatric volunteers.Methods: An in vitro whole blood endotoxin stimulation model using 1 mg/ml lipopolysaccharide (LPS) over a 24 h time period was used to investigate the effects of 2 mM glutamine on HSP70 and inflammatory mediator release in healthy children. Results: The addition of 2 mM glutamine to the incubation media significantly increased HSP70 release over time (p < 0.05). This was associated with an early pro-inflammatory effect on TNF-a release at 4 h (p < 0.005) which was not seen at 24 h. There was a non significant trend towards higher levels of IL-6 and IL-10 following the addition of 2 mM glutamine, which appears to differ from the response reported in adult and animal models.Conclusion: Glutamine supplementation of incubation media promotes HSP70 and early TNF-a release in an in vitro model using blood samples from healthy children.

show abstract

Section: Discussionsupporting

confidence: 60%

The effect of 2 mMol glutamine supplementation on HSP70 and TNF-α release by LPS stimulated blood from healthy children

et al. 2015

View full text Add to dashboard Cite

show abstract

“…DnaK can induce the production of IL-10 in dendritic cells [14], macrophages [10], and synovial cells from arthritis patients [33]. Moreover, in M. tuberculosis (Mtb) DnaK can modulate the production of pro-inflammatory cytokines via IL-10 in murine dendritic cells [34].…”

Section: Discussionmentioning

confidence: 99%

IL-10 is required for polarization of macrophages to M2-like phenotype by mycobacterial DnaK (heat shock protein 70)

Lopes

Borges

Zanin³

et al. 2016

Cytokine

Self Cite

View full text Add to dashboard Cite

“…Therapeutic modulation of cytokine expression offers the possibility of major changes in adipose tissue physiology. Adipocytes can synthesize some inflammatory cytokines such as interleukin-6 (IL-6), tumor necrosis factor-alpha (TNF-α), and interleukin-1beta (IL-1β) (Coppack 2001;Kern et al 2001;Wu et al 2007), and possible relationships between Hsp70 and inflammatory cytokine expression were observed in some studies (Asea et al 2000;Borges et al 2013;Van Molle et al 2002). It was also reported that restraint stress induced Hsp70 in adipose tissue (Yamamoto et al 2002).…”

Section: Introductionmentioning

confidence: 99%

Effect of resistance exercise training on expression of Hsp70 and inflammatory cytokines in skeletal muscle and adipose tissue of STZ-induced diabetic rats

Shamsi

Mahdavi

Quinn

et al. 2016

Cell Stress and Chaperones

View full text Add to dashboard Cite

Impairment of adipose tissue and skeletal muscles accrued following type 1 diabetes is associated with protein misfolding and loss of adipose mass and skeletal muscle atrophy. Resistance training can maintain muscle mass by changing both inflammatory cytokines and stress factors in adipose tissue and skeletal muscle. The purpose of this study was to determine the effects of a 5-week ladder climbing resistance training program on the expression of Hsp70 and inflammatory cytokines in adipose tissue and fast-twitch flexor hallucis longus (FHL) and slow-twitch soleus muscles in healthy and streptozotocin-induced diabetic rats. Induction of diabetes reduced body mass, while resistance training preserved FHL muscle weight in diabetic rats without any changes in body mass. Diabetes increased Hsp70 protein content in skeletal muscles, adipose tissue, and serum. Hsp70 protein levels were decreased in normal and diabetic rats by resistance training in the FHL, but not soleus muscle. Furthermore, resistance training decreased inflammatory cytokines in FHL skeletal muscle.On the other hand, Hsp70 and inflammatory cytokine protein levels were increased by training in adipose tissue. Also, significant positive correlations between inflammatory cytokines in adipose tissue and skeletal muscles with Hsp70 protein levels were observed. In conclusion, we found that in diabetic rats, resistance training decreased inflammatory cytokines and Hsp70 protein levels in fast skeletal muscle, increased adipose tissue inflammatory cytokines and Hsp70, and preserved FHL muscle mass. These results suggest that resistance training can maintain skeletal muscle mass in diabetes by changing inflammatory cytokines and stress factors such as Hsp70 in skeletal muscle and adipose tissue.

show abstract

Extracellular Hsp70 inhibits pro-inflammatory cytokine production by IL-10 driven down-regulation of C/EBPβ and C/EBPδ

Abstract: C/EBPβ and C/EBPδ transcription factors are inhibited by Hsp70 treatment, and their inhibition occurs via the TLR2-ERK-STAT3-IL-10 pathway in BMDCs, mediating the anti-inflammatory effects of Hsp70.

Cited by 46 publications

References 62 publications

The effect of 2 mMol glutamine supplementation on HSP70 and TNF-α release by LPS stimulated blood from healthy children

The effect of 2 mMol glutamine supplementation on HSP70 and TNF-α release by LPS stimulated blood from healthy children

IL-10 is required for polarization of macrophages to M2-like phenotype by mycobacterial DnaK (heat shock protein 70)

Effect of resistance exercise training on expression of Hsp70 and inflammatory cytokines in skeletal muscle and adipose tissue of STZ-induced diabetic rats

Contact Info

Product

Resources

About