Extracellular Hydrolytic Enzymes of Yeasts Isolated From Fruit and Beet Peels in Algeria

Abstract

Fourty-two yeast strains were isolated from natural sources in Algeria. Based on the sequence analysis of the 26S ribosomal RNA D1/D2 domain they were identified to be of 8 species belonging to the genera Aureobasidium, Candida, Clavispora, Hanseniaspora, Pichia, Rhodotorula and Vishniacozyma. All yeast isolates were screened for cellulase, amylase, protease and lipase production. Six strains of Aureobasidium pullulans, Rhodotorula diobovata and Vishniacozyma tephrensis demonstrated ability to produce at least one extracellular enzyme. The enzyme activity index (EAI) for cellulase was noted to be prominent in the isolates of A. pullulans (A1, A3, A5) and V. tephrensis A4 as 2.3 and 2.1, respectively. Highest EAI for amylase and protease was also seen in A. pullulans isolate A1 (EAI = 2.9) and isolate A3 (EAI = 1.9), respectively. For lipase, the EAI was superior in V. tephrensis A4 (EAI = 1.5) when compared to the isolates of R. diobovata (B1, O5) (EAI =1.4) and A. pullulans A5 (EAI =1.3). To the best of our knowledge, this is the first report of cellulase and/or lipase activity in V. tephrensis and R. diobovata strains associated with apple, orange and beet peels in Algeria. Furthermore, the strain A. pullulans A5 showed enzymatic activities for all the enzymes screened in the current work. Thus, our study can provide further information about the diversity and enzyme production by yeasts and demonstrated the potential for yeast isolated from fruit and beet peels as sources for extracellular hydrolytic enzymes.

Inventoried Yeast Species in Algeria

“…Pichia guilliermondii) and C. tropicalis were isolated from potatoes [86]. Recently, Aureobasidium pullulans, Rhodotorula diobovata, Vishniacozyma tephrensis were isolated from fruits and beet peels and showed extracellular enzyme synthesis [87].…”

“…Twelve yeast species are tested to produce varied enzymes and also to valorise different substrates as well as fruit wastes. Aureobasidium pullulans on tomato pomace produces polygalacturonase [68] and also Cellulase, Amylase, protease, and lipase in an experimental purpose valorising fruit and beet peels [87]. Lipase is produced by Candida boidinii isolated from olive pomace [84], Ustilago cynodontis isolated from saline soil [71], Vishniacozyma tephrensis [87] and Yarrowia lipolytica [84].…”

“…Aureobasidium pullulans on tomato pomace produces polygalacturonase [68] and also Cellulase, Amylase, protease, and lipase in an experimental purpose valorising fruit and beet peels [87]. Lipase is produced by Candida boidinii isolated from olive pomace [84], Ustilago cynodontis isolated from saline soil [71], Vishniacozyma tephrensis [87] and Yarrowia lipolytica [84]. α-Amylase is produced by C. glabrata isolated from saline soil and valorising whey [75], C. guillierrnondii and C. tropicalis [86], Clavispora lusitaniae [88, 140], Schwanniomyces sp.…”

“…[67] and Ustilago cynodontis [71]. Cellulase is produced by A. pullulans [87] and U. cynodontis [71]. The protease is produced by A. pullulans [87], Cr.…”

“…Cellulase is produced by A. pullulans [87] and U. cynodontis [71]. The protease is produced by A. pullulans [87], Cr. neoformans [74], and U. cynodontis [71].…”