Extracellular keratinase from Trichophyton sp. HA-2 isolated from feather dumping soil

Anbu, Periasamy; Amanda, Hilda; Sur, Hwal-Won; Hur, Byung‐Ki; Jayanthi, S.

doi:10.1016/j.ibiod.2007.07.017

Cited by 51 publications

(27 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Table 3 and Figure 1 show that the high keratinase activity as a clear zone around the colony (54, 65, 50, 58 and 60 mm) ( 25,18,20,25,15,23 and 21 mm), respectively. While Alternaria alternate and Curvularia lunata had negative keratinase activity The results agree to some extend with previous findings (Anbu et al, 2008;Awasthi and Kushwaha, 2011;Ramakrishnaiah et al, 2013;Kumar and Kushwaha, 2014;Singh, 2014;Singh et al, 2016;Bohacz, 2017).…”

Section: Saprophytic Fungi (Using Glucose-czapek's Agar)supporting

confidence: 91%

Mycoflora associated with the goats hair and sheep wool in Taif, Saudi Arabia

Awad¹

2017

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

The objective of this study was to evaluate the occurrence of mycoflora in 30 samples of healthy goat's hair and sheep wool collected from different localities in Taif, Saudi Arabia, and the ability of some fungal isolates for keratinase activities. Sixty four species belonging to 28 genera were collected from the two substrates. The wool of sheep was polluted with fungi than goat hairs, and contained high total counts and number of fungal genera and species. Nine species of true dermatophytes isolated belonged to Microsporum (3 species) and Trichophyton (6 species). Several keratinophilic species were isolated of which, Chrysosporium indicum, Chrysosporium keratinophilum and Chrysosporium tropicum were the most prevalent. The commonest saprophytes in order of frequency were members of the genera, Aspergillus, Penicillium, Alternaria and Cochliobolus. In addition, the other genera found included Acremonium, Chaetomum, Cladosporium, Cochliobolus, Fusarium, Mucor, Paecilomyces, Phoma, Rhizopus, Scopulariopsis, Stachybotrys, Trichoderma and others. Five species from 20 tested isolates (Aspergillus niger, C. keratinophilum, C. tropicum, Microsporum gypseum and Trichoderma viride) had high keratinase activity. The results of this study indicate that both goat hair and sheep wool provide a suitable habitat for dermatophytes and other keratinophilic fungi. Most of these fungi play an important role in the degradation of keratin substrates, so that they can help preserve the environment and reduce pollution.

show abstract

Section: Saprophytic Fungi (Using Glucose-czapek's Agar)supporting

confidence: 91%

Mycoflora associated with the goats hair and sheep wool in Taif, Saudi Arabia

Awad¹

2017

Afr. J. Microbiol. Res.

View full text Add to dashboard Cite

show abstract

“…Among fungi, keratinolytic activity from dermatophytes has received more attention due to medical and veterinary implications [28,37]. Although some nondermatophytic fungi have already been described [1,2,10,14,15,19,21,33], for biotechnological purposes, bacterial keratinases have been received more attention, particularly those produced by Bacillus [5,13,20,36]. Keratinolytic enzymes Wnd use in environment friendly biotechnological processes that use keratin-containing wastes from poultry industry [16,29].…”

Section: Introductionmentioning

confidence: 99%

Influence of the carbon and nitrogen sources on keratinase production by Myrothecium verrucaria in submerged and solid state cultures

Gioppo¹,

Moreira-Gasparin²,

Costa³

et al. 2009

J Ind Microbiol Biotechnol

View full text Add to dashboard Cite

Myrothecium verrucaria is a nondermatophytic filamentous fungus able to grow and to produce keratinase in submerged (93.0 +/- 19 U/ml) and solid state (98.8 +/- 7.9 U/ml) cultures in which poultry feather powder (PFP) is the only substrate. The purpose of the present work was to verify how different carbon and nitrogen sources can influence the production of keratinase by this fungus. Addition of carbohydrates, such as glucose and sucrose, caused only slight improvements in keratinase production, but the addition of starch caused a significant improvement (135.0 +/- 25 U/ml). The highest levels of keratinase activity, however, were obtained by supplementing the PFP cultures with cassava bagasse, 168.0 +/- 28 U/ml and 189.0 +/- 26 U/ml in submerged and solid state cultures, respectively. Contrarily, the supplementation of PFP medium with organic or inorganic nitrogen sources, such as casein, soy bean protein, gelatin, ammonium nitrate and alanine, decreased the production of keratinase in both types of cultures (around 20 U/ml), showing that the production of keratinase by M. verrucaria is repressed by nitrogen sources. The results obtained in this work suggest that the association of the two residues PFP plus cassava bagasse could be an excellent option as a cheap culture medium for the production of keratinase in submerged and solid state cultures.

show abstract

“…In the study of extracellular keratinase from Trichophyton sp. detected the highest enzyme production was registered at 35°C (5.0 KU/ml) [27]. In other study Anbu et al [28] detected the highest keratinase activity by Scopulariopsis brevicaulis (3.2 KU/mL) and Trichophyton mentagrophytes (2.7 KU/mL) in the culture medium with chicken feathers during 5 week of cultivation.…”

Section: Resultsmentioning

confidence: 82%

Biodegradation of Feather by Microsporum fulvum Singly or in Combination with Other Fungi

Singh¹

2014

J Bioremed Biodeg

View full text Add to dashboard Cite

The course of feather degradation by Microsporum fulvum singly and in combination with Chrysosporium tropicum, C. vallenarense, C. quenslandicum and Microsporum boullardii was studied by measuring protein, amino acids, keratinase enzyme released in the culture medium and weight loss of feather by M. fulvum and the effect of addition of M. fulvum on continued degradation of feather by C. tropicum, C. vallenarense, C. quenslandicum and M. boullardii were also studied. The synergistic action of M. fulvum and C. tropicum on feather degradation was found to be more effective. The biodegradation ability of M. fulvum can be effective by C. tropicum and C. vallenarense if the latter fungi join midway of keratinolysis. M. fulvum was also effective on the biodegradation ability of C. tropicum. M. fulvum did not act as follower fungus in wool degradation. Journal of Bior emediation & Biodegradation

show abstract

Extracellular keratinase from Trichophyton sp. HA-2 isolated from feather dumping soil

Cited by 51 publications

References 34 publications

Mycoflora associated with the goats hair and sheep wool in Taif, Saudi Arabia

Mycoflora associated with the goats hair and sheep wool in Taif, Saudi Arabia

Influence of the carbon and nitrogen sources on keratinase production by Myrothecium verrucaria in submerged and solid state cultures

Biodegradation of Feather by Microsporum fulvum Singly or in Combination with Other Fungi

Contact Info

Product

Resources

About