Extracellular L-Asparaginase from Streptomyces labedae VSM-6: Isolation, Production and Optimization of Culture Conditions Using RSM

Abstract

Objective: The present study was intended to isolate actinomycetes VSM-6 from deep sea sediment samples of Bay of Bengal that is potent to produce L -asparaginase. Materials and Methods: The identification of the isolate was executed by polyphasic taxonomy.Optimization was carried out using one factor at a time (O-F-A-T) for the production of the L-asparaginase. RSM was pledged to optimize the L -asparaginase production by S.labedae VSM-6. Central composite design was applied to study the influence of the variables and their interactive effects on the production of L -asparaginase. Unstructured Kinetic modelling for L -asparaginase production was adopted using Leudeking-Piret (LILP) and Logistic Incorporated Modified Leudeking-Piret (LIMLP) models. Results: Optimization using One-Factor-At-A-time registered a turnout of 8.92 IU/ml of L -asparaginase production. But results obtained from the statistical design are in agreement with the experimental results. The model followed the second order polynomial equation and the model adequacy was determined by the P value (<0.0001), Coefficient determination (R 2 ) with a value of 0.9942 and the adjusted R 2 = 0.9087 which determines that the model was significant. The experimental values are in compliance with the model anticipated values and catalogued an escalation in yield of L -asparaginase (10.17 IU/ml) by RSM. Unstructured Kinetic modelling for L -asparaginase production adopting Leudeking-Piret (LILP) and Logistic Incorporated Modified Leudeking-Piret (LIMLP) models showed L -asparaginase production of (10.17 IU/ml), closer to model anticipated value (10.23 IU/ml) so unstructured models provided a better approximation for L -asparaginase production by S.labedae VSM-6. Conclusion: From our study we have reported for the first time the production of L -asparaginase from S.labedae VSM-6 using central composite design and kinetic modelling.

Production and Characterization of L-Asparaginases of Streptomyces Isolated from the Arauca Riverbank (Colombia)

“…The X0 value was obtained experimentally as a reference from the initial aggregated bacteria to the experiment; and through this initial value and a Matlab iteration, a theoretical biomass result was obtained to get a value similar to the experimental data. The proposed logistic model was adequate to explain the sigmoidal profile of bacterial growth as reported previously [37].…”

“…Liu and Zajic in 1972 reported that lactose was the best carbon source to optimize the production of L-asparaginase from Erwina aroideae [52], however, there are few studies that reported lactose as the best carbon source for Streptomyces. In previous studies, it has been reported that L-asparagine is a suitable nitrogen source for the production of L-asparaginase by Streptomyces venezuelae, S. karnatakensis, S. collinus, S. gulbargensis and P. endphytica [37]. Several studies have shown that when using the combination of two nitrogen sources in the medium, the production of L-asparaginase increases [37,53].…”

“…In previous studies, it has been reported that L-asparagine is a suitable nitrogen source for the production of L-asparaginase by Streptomyces venezuelae, S. karnatakensis, S. collinus, S. gulbargensis and P. endphytica [37]. Several studies have shown that when using the combination of two nitrogen sources in the medium, the production of L-asparaginase increases [37,53]. In the present study, it was observed that the microorganisms had a higher production of L-asparaginase in the media containing malt extract and L-asparagine for all sevens strains (Fig.…”

“…(2) Also, the m and n kinetic constants of the Luedeking-Piuret model for the production of L-asparaginase were graphically calculated [36,37].…”

Process development for scale-up production of a therapeutic L-asparaginase by Streptomyces brollosae NEAE-115 from shake flasks to bioreactor

“…Mangamuri et al . 40 used one factor at a time and CCD to evaluate the effect of various physico-chemical factors on L-asparaginase production by Streptomyces labedae VSM-6. Maximum L-asparaginase production (8.92 U/mL) by Streptomyces labedae VSM-6 using conventional one factor at a time optimization was found after 6 th day of incubation in production medium supplemented with 1% L-asparagine, 1.5% starch, 1% yeast extract at initial pH 8 after 144 h of incubation.…”