Extracellular matrix

Labat‐Robert, J.; Bihari-Varga, M; Robert, L.

doi:10.1016/0014-5793(90)81291-u

Cited by 103 publications

(52 citation statements)

References 57 publications

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“…The ECM consists of four major macromolecules-collagen, elastin, structural glycoproteins, and proteoglycans-and acts not only as a physical scaffold, but also as a crucial modulator of biologic processes, including differentiation, development regeneration, tumor progression, and repair. 58,59 Chronic inflammation is less uniform histologically compared to acute inflammation, and the wound healing response is generally dependent on the extent or degree of injury. Figure 1A is typical of the acute inflammatory response (day 3), with a large infiltration of neutrophils into the local site.…”

Section: -48mentioning

confidence: 99%

A Review of the Biocompatibility of Implantable Devices: Current Challenges to Overcome Foreign Body Response

Onuki

Bhardwaj

Papadimitrakopoulos

et al. 2008

J Diabetes Sci Technol

347

282

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In recent years, a variety of devices (drug-eluting stents, artificial organs, biosensors, catheters, scaffolds for tissue engineering, heart valves, etc.) have been developed for implantation into patients. However, when such devices are implanted into the body, the body can react to these in a number of different ways. These reactions can result in an unexpected risk for patients. Therefore, it is important to assess and optimize the biocompatibility of implantable devices. To date, numerous strategies have been investigated to overcome body reactions induced by the implantation of devices. This review focuses on the foreign body response and the approaches that have been taken to overcome this. The biological response following device implantation and the methods for biocompatibility evaluation are summarized. Then the risks of implantable devices and the challenges to overcome these problems are introduced. Specifically, the challenges used to overcome the functional loss of glucose sensors, restenosis after stent implantation, and calcification induced by implantable devices are discussed.

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Section: -48mentioning

confidence: 99%

A Review of the Biocompatibility of Implantable Devices: Current Challenges to Overcome Foreign Body Response

Onuki

Bhardwaj

Papadimitrakopoulos

et al. 2008

J Diabetes Sci Technol

347

282

View full text Add to dashboard Cite

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“…Fibronectin mediates cell interactions with the ECM, and affects a range of cell functions including cell adhesion, cell migration, differentiation, haemostasis, phagocytosis and chemotaxis (Refs 40,78). Present at low levels in normal tendon, fibronectin is massively increased after tendon injury and consequently has been implicated in cell adhesion, migration and differentiation at the site of injury (Refs 79,80,81).…”

Section: Glycoproteinsmentioning

confidence: 99%

Chronic tendon pathology: molecular basis and therapeutic implications

Riley

2005

Expert Rev. Mol. Med.

103

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Tendons are frequently affected by chronic pain or rupture. Many causative factors have been implicated in the pathology, which until relatively recently was under-researched and poorly understood. There is now a greater knowledge of the molecular basis of tendon disease. Most tendon pathology (tendinopathy) is associated with degeneration, which is thought to be an active, cell-mediated process involving increased turnover and remodelling of the tendon extracellular matrix. Degradation of the tendon matrix is mediated by a variety of metalloproteinase enzymes, including matrix metalloproteinases and 'aggrecanases'. Neuropeptides and other factors released by stimulated cells or nerve endings in or around the tendon might influence matrix turnover, and could provide novel targets for therapeutic intervention.Tendons are dense, fibrous connective tissues that connect muscle to bone and are essential for the transmission of force and the generation of movement at a joint. They are highly ordered composite materials consisting of collagens, proteoglycans and various glycoproteins, many of which have been poorly characterised. Tendon problems such as tendon rupture and chronic tendon pain are common, although the underlying pathology is not well understood and the conditions are often difficult to treat (Ref.

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“…Cells cultured as monolayers ( Fig 1A) showed an increase in values of total protein with increasing serum concentrations (0.73±0.25 cpmxlO 6 / cellsxlO 6 versus 2.6±1.0 cpmxlO 6 /cellsxlO 6 ; mean of three animals). Cells within a collagen lattice (Fig IB), however, showed no differences in values of total protein synthesis when cultivated either under low (0.55±0.24 cpmxl0 6 /cellsxl0 6 ; mean of three animals) or high (0.85+0.39 cpmxlO'/cellsxlO 6 ; mean of three animals) serum concentrations. Lattice cultures showed comparable values of total protein synthesis with monolayer cultures supplemented with 0.5% FCS.…”

Section: Stimulation Of Lattice-cultured Smcs By Fcsmentioning

confidence: 89%

“…Lattice-cultured SMCs and monolayercultured SMCs synthesized collagen under low and high serum concentration. Monolayer cultures (Fig 1C) showed an increase in collagen synthesis with an increase in serum concentration (1.55±0.53 cpmxlO 5 / cellsxlO 6 versus 2.71±1.14 cpmxlO 5 /cellsxlO 6 ; mean of three animals). SMCs within collagen lattices ( Fig ID) Combining the values for total collagen synthesis with those for total protein synthesis resulted in the proportions of collagen in protein synthesized.…”

Section: Stimulation Of Lattice-cultured Smcs By Fcsmentioning

confidence: 95%

Responsiveness of aortic smooth muscle cells to soluble growth mediators is influenced by cell-matrix contact.

Thie

Harrach

Schönherr

et al. 1993

Arterioscler Thromb

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Excessive proliferation and overexpresslon of collagens by smooth muscle cells (SMCs) are important features of atherogenesis. To understand the role of the extracellular matrix in the regulation of these processes, we examined proliferation and protein/collagen synthesis of SMCs in contact with a collagen matrix. Adult pig SMCs were isolated from the aortic media by collagenase digestion, subcultured as monolayers, and then embedded into a three-dimensional network of type I collagen, ie, a collagen lattice. Cells were subsequently exposed to growth-promoting media, and their behavior was observed in comparison with monolayer cultures on plastic Treatment of monolayers with increasing concentrations of fetal calf serum resulted in activation of the cell cycle, onset of cell proliferation, and increased protein/collagen synthesis. In contrast, similar treatment of collagen lattice-cultured SMCs failed to influence cell proliferation and protein/collagen synthesis. However, stimulation of proliferation of lattice-cultured SMCs by platelet-derived growth factor-A/B was feasible; nevertheless, the rate of proliferation was modest compared with monolayers. In addition, the onset of proliferation was accompanied by a decrease in collagen synthesis of the cells. Thus, a collagenous matrix appears to suppress the responsiveness of SMCs to soluble growth mediators. It is speculated that interactions between SMCs and the extracellular matrix may modify proliferation and protein/collagen synthesis of cells not only in vitro but also in vivo during atherogenesis by making and breaking binding sites between extracellular collagen and matrix receptors.

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Extracellular matrix

Cited by 103 publications

References 57 publications

A Review of the Biocompatibility of Implantable Devices: Current Challenges to Overcome Foreign Body Response

A Review of the Biocompatibility of Implantable Devices: Current Challenges to Overcome Foreign Body Response

Chronic tendon pathology: molecular basis and therapeutic implications

Responsiveness of aortic smooth muscle cells to soluble growth mediators is influenced by cell-matrix contact.

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