Extracellular matrix components produced by SV40-transformed human epidermal keratinocytes

Kw, Brown; Ek, Parkinson

doi:10.1002/ijc.2910330215

Cited by 29 publications

(27 citation statements)

References 40 publications

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“…However, these cells do show certain novel quantitative changes in their ECM when compared to normal keratinocytes, the most consistent of these changes being (1) a decrease in entactin production and (2) a shift in glycosaminoglycan (GAG) production from hyaluronic acid to heparan sulphate (Brown & Parkinson, 1984;.…”

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confidence: 97%

“…We have recently demonstrated that both SV40-transformed human keratinocytes and keratinocytes derived from human squamous cell carcinomas (SCCs) retain the ability to produce an extracellular matrix (ECM) (Brown & Parkinson, 1984;. However, these cells do show certain novel quantitative changes in their ECM when compared to normal keratinocytes, the most consistent of these changes being (1) a decrease in entactin production and (2) a shift in glycosaminoglycan (GAG) production from hyaluronic acid to heparan sulphate (Brown & Parkinson, 1984;.…”

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confidence: 99%

“…This decrease in sulphation reduces the affinity of heparan sulphate for fibronectin (Stamatoglou & Keller, 1983;Robinson et al, 1984) and may therefore interfere with the complex interactions between the various glycoprotein and proteoglycan components of the ECM, in which heparan sulphate is thought to play a central role (Gallagher et al, 1986 (Rheinwald, 1980), as previously described (Brown & Parkinson, 1983). The cells used in this study were: normal strains Z and R (derived from newborn foreskin and the foreskin of a 10-year-old respectively); SV6-1 Bam/HFK, an SV40 transformed keratinocyte cell line (Brown & Parkinson, 1984;Brown & Gallimore, manuscript (Rheinwald & Beckett, 1981). The properties of all these keratinocytes have been described in detail elsewhere (Brown & Parkinson, 1984 and reference therein).…”

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confidence: 99%

“…The cells used in this study were: normal strains Z and R (derived from newborn foreskin and the foreskin of a 10-year-old respectively); SV6-1 Bam/HFK, an SV40 transformed keratinocyte cell line (Brown & Parkinson, 1984;Brown & Gallimore, manuscript (Rheinwald & Beckett, 1981). The properties of all these keratinocytes have been described in detail elsewhere (Brown & Parkinson, 1984 and reference therein).…”

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confidence: 99%

“…Seven day-old cultures of keratinocytes were metabolically labelled with 3H-glucosamine and/or 35SO2-as previously described (Brown & Parkinson, 1984 and further purified by pronase digestion and ethanol precipitation in the presence of carriers GAGs, exactly as described previously (Brown & Parkinson, 1984). Nitrous acid treatment was by the method of Wusteman (1979), as described previously (Brown & Parkinson, 1983).…”

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Sulphation and fibronectin-binding properties of heparan sulphate glycosaminoglycans from transformed cultured human keratinocytes

Kw¹

1987

Br J Cancer

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confidence: 97%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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confidence: 99%

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Sulphation and fibronectin-binding properties of heparan sulphate glycosaminoglycans from transformed cultured human keratinocytes

Kw¹

1987

Br J Cancer

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Changes in fibronectin synthesis and binding distribution in SV40‐transformed human keratinocytes

Edelman

Steinberg

Defendi

1985

Intl Journal of Cancer

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We have studied the synthesis and distribution of fibronectin in human epidermal keratinocytes infected by SV40, a system in which the acquisition of transformed properties occurs in a sequential and progressive manner. Immunofluorescence studies showed that cultured uninfected keratinocytes do not exhibit fibronectin on the superficial cell surface, but that virus-infected cells come to display superficial fibronectin-containing cables in a density-dependent manner after a certain point in the transformation process. In contrast, organized arrays of fibronectin-containing fibrils associated with the cell-substrate attachment complex were seen in uninfected keratinocytes and in virus-infected cells at all stages of the transformation process. Studies of fibronectin synthesis using metabolic labelling of cell proteins with 35S methionine showed that viral infection caused a striking increase in overall fibronectin synthesis, although with a much higher proportion of newly synthesized fibronectin being secreted into the cell culture medium than in the case of the uninfected cells.

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Alteration of the extracellular matrix of cultured human keratinocytes by transformation and during differentiation

Brown

Parkinson

1985

Intl Journal of Cancer

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We have investigated the production of 3 extracellular matrix proteins (fibronectin, laminin and entactin) and glycosaminoglycans (GAGs) by keratinocytes derived from human squamous-cell carcinomas (SCCs). All the SCC lines retained the ability to synthesize fibronectin, laminin and entactin, and to incorporate them into an extracellular matrix. In some of the SCC lines fibronectin production was higher than in normal keratinocytes, and in most lines laminin production was equal to or higher than that seen in normal keratinocyte strains. All the tumorigenic SCC lines produced less entactin than normal keratinocytes. Five out of 7 SCC lines showed a shift in GAG production compared with normal keratinocytes, so that in these lines heparan sulphate was the major GAG as opposed to hyaluronic acid in the normal keratinocytes. As these changes could have been a consequence of the reduced differentiation found in the SCC lines, we fractionated normal keratinocytes into 4 size classes corresponding to different stages of differentiation, using Percoll gradient centrifugation. It was found that the ability to produce these extracellular matrix glycoproteins and GAGs was lost as keratinocytes terminally differentiated. However, this did not explain the matrix changes seen in the SCC lines, since the undifferentiated normal keratinocytes produced a normal pattern of extracellular matrix components. Nonetheless, the loss of extracellular matrix production may well explain the reduction in substratum adhesiveness which occurs as keratinocytes terminally differentiate.

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Extracellular matrix components produced by SV40-transformed human epidermal keratinocytes

Cited by 29 publications

References 40 publications

Sulphation and fibronectin-binding properties of heparan sulphate glycosaminoglycans from transformed cultured human keratinocytes

Sulphation and fibronectin-binding properties of heparan sulphate glycosaminoglycans from transformed cultured human keratinocytes

Changes in fibronectin synthesis and binding distribution in SV40‐transformed human keratinocytes

Alteration of the extracellular matrix of cultured human keratinocytes by transformation and during differentiation

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