Extracellular Matrix Expression, Organization, and Interaction with Heart Myocytes During Development and Disease

Carver, Wayne; Burgess, Maria Lonnett; Jyring, R; Terracio, Louis; Borg, Thomas K.

doi:10.1007/978-3-642-77891-9_10

Cited by 2 publications

(1 citation statement)

References 40 publications

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“…Besides lithography and microcontact printing, electrospinning is another spatial patterning method that uses electrostatic forces to draw a homogeneous mixture of polymer and volatile solvent to produce small diameter nanoscale or microscale fibrous scaffolds [96][97][98]. The nanofibrous fibers ranging in diameter from tens to hundreds of nanometers mimic the ECM of native cardiac tissue [99,100]. Culturing native or stem cell-derived CMs onto electrospun substrates produce aligned cells that mimic the anisotropy of the native myocardium (Figure 1).…”

Section: Spatial Factorsmentioning

confidence: 99%

Regulation of the Microenvironment for Cardiac Tissue Engineering

Wanjare

Huang

2017

Regen. Med.

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The microenvironment of myocardium plays an important role in the fate and function of cardiomyocytes (CMs). Cardiovascular tissue engineering strategies commonly utilize stem cell sources in conjunction with microenvironmental cues that often include biochemical, electrical, spatial and biomechanical factors. Microenvironmental stimulation of CMs, in addition to the incorporation of intercellular interactions from non-CMs, results in the generation of engineered cardiac constructs. Current studies suggest that use of these factors when engineering cardiac constructs improve cardiac function when implanted in vivo. In this review, we summarize the approaches to modulate biochemical, electrical, biomechanical and spatial factors to induce CM differentiation and their subsequent organization for cardiac tissue engineering application.

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Section: Spatial Factorsmentioning

confidence: 99%

Regulation of the Microenvironment for Cardiac Tissue Engineering

Wanjare

Huang

2017

Regen. Med.

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Integrin-mediated collagen gel contraction by cardiac fibroblasts. Effects of angiotensin II.

Burgess

Carver

Terracio

et al. 1994

Circulation Research

104

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Angiotensin II (Ang II), a vasoactive octapeptide, has been implicated in cardiac growth and the development of hypertrophy and fibrosis secondary in hypertensive disease. These consequences of Ang II imply an effect on the function and morphology of cardiac interstitial cells (fibroblasts). The present investigation was designed to (1) determine whether neonatal heart fibroblasts (NHFs) possess functional Ang II receptors on their plasma membrane and (2) examine the effects of Ang II on NHFs in vitro using three- and two-dimensional (3D and 2D, respectively) cultures. Several analytic techniques were used to test the specific questions of the present study. Since cardiac fibroblast phenotype can be influenced by culture conditions, both 2D and 3D cultures were used in the present investigations. Reverse-transcriptase polymerase chain reaction and radioligand binding analysis were used to test for the presence of Ang II receptors on NHFs. Both revealed that NHFs in 2D culture possess Ang II receptor mRNA and Ang II receptors. When isolated NHFs were cultured in 3D collagen gels and treated with Ang II, gel contraction was stimulated by NHFs. This effect was attenuated by the specific Ang II receptor antagonist [Sar1,Ala8]Ang II. Ang II-stimulated gel contraction was completely inhibited by extracellular matrix receptor (beta 1-integrin) antibodies (P < .05), supporting previous studies indicating that collagen gel contraction is mediated via the integrins. Immunofluorescent staining was used to test the localization of cell-surface integrins. A more intense staining pattern for beta 1-integrin in Ang II-treated versus control cells was observed.(ABSTRACT TRUNCATED AT 250 WORDS)

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Extracellular Matrix Expression, Organization, and Interaction with Heart Myocytes During Development and Disease

Cited by 2 publications

References 40 publications

Regulation of the Microenvironment for Cardiac Tissue Engineering

Regulation of the Microenvironment for Cardiac Tissue Engineering

Integrin-mediated collagen gel contraction by cardiac fibroblasts. Effects of angiotensin II.

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