Extracellular matrix mimicking scaffold promotes osteogenic stem cell differentiation: A new approach in osteoporosis research

Moll, Concepción; Schmiedinger, Thomas; Moll, Matthias; Seppi, Thomas; Pfaller, Kristian; Hess, Michael W.; Gutleben, Karin; Lindtner, Richard A.; Blauth, M.; Krumschnabel, Gerhard; Ebner, Hannes

doi:10.3233/bme-171659

Cited by 2 publications

(2 citation statements)

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“…Electrospun gelatin scaffolds, which demonstrate structural properties similar to native collagen, have been shown to promote MSC proliferation, survival, and osteogenic differentiation in the absence of exogenous growth factors (Chang et al, 2012). Following 21 days of osteogenic induction, BM-MSCs seeded in pure gelatin scaffolds demonstrated significantly increased mineralization relative to 2D controls (Moll et al, 2017). Poly(ǫcaprolactone) (PCL) is used commonly in general scaffold design owing to its biocompatibility, biodegradability, low immune reactivity, optimal biomechanical properties and the ability to form complex 3D shapes; however, its usage in osteogenic induction is limited as it lacks the surface reactivity necessary for cell attachment.…”

Section: Opmentioning

confidence: 99%

The Role of Chronic Inflammatory Bone and Joint Disorders in the Pathogenesis and Progression of Alzheimer's Disease

Culibrk

Hahn

2020

Front. Aging Neurosci.

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Late-onset Alzheimer's Disease (LOAD) is a devastating neurodegenerative disorder that causes significant cognitive debilitation in tens of millions of patients worldwide. Throughout disease progression, abnormal secretase activity results in the aberrant cleavage and subsequent aggregation of neurotoxic Aβ plaques in the cerebral extracellular space and hyperphosphorylation and destabilization of structural tau proteins surrounding neuronal microtubules. Both pathologies ultimately incite the propagation of a disease-associated subset of microglia—the principle immune cells of the brain—characterized by preferentially pro-inflammatory cytokine secretion and inhibited AD substrate uptake capacity, which further contribute to neuronal degeneration. For decades, chronic neuroinflammation has been identified as one of the cardinal pathophysiological driving features of AD; however, despite a number of works postulating the underlying mechanisms of inflammation-mediated neurodegeneration, its pathogenesis and relation to the inception of cognitive impairment remain obscure. Moreover, the limited clinical success of treatments targeting specific pathological features in the central nervous system (CNS) illustrates the need to investigate alternative, more holistic approaches for ameliorating AD outcomes. Accumulating evidence suggests significant interplay between peripheral immune activity and blood-brain barrier permeability, microglial activation and proliferation, and AD-related cognitive decline. In this work, we review a narrow but significant subset of chronic peripheral inflammatory conditions, describe how these pathologies are associated with the preponderance of neuroinflammation, and posit that we may exploit peripheral immune processes to design interventional, preventative therapies for LOAD. We then provide a comprehensive overview of notable treatment paradigms that have demonstrated considerable merit toward treating these disorders.

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Section: Opmentioning

confidence: 99%

The Role of Chronic Inflammatory Bone and Joint Disorders in the Pathogenesis and Progression of Alzheimer's Disease

Culibrk

Hahn

2020

Front. Aging Neurosci.

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“…Thus, a detailed analysis of the mineralized nodules formed in cell culture medium should be performed, in order to assess the interaction between different types of cells and the components in the culture medium [31][32][33][34].…”

Section: Introductionmentioning

confidence: 99%

Interaction of stem cells derived from maxillary and mandibular bone with salts from culture medium

Ilea¹,

Timuș²,

Şoriţău³

et al. 2019

Front Nanosci Nanotech

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Developing stem cells require specific culture media for each cell culture. Unexpected chemical reactions may occur between the components of the culture medium and the products secreted by the stem cells.In this study we used two human bone fragments: mandibular alveolar bone and tuberosity bone. Mesenchymal stem cells derived from the bone tissues were isolated using the explant method and cultured in specific culture medium DMEM/F-12 (Dulbecco's modified Eagle's medium / Nutrient mixture F-12). Cell cultures were maintained at a humidity of 99%, a temperature of 37 °C and 5% CO 2 . The bone stem cells were examined by optical inverted microscopy and scanning electron microscopy with energy-dispersive X-ray spectroscopy (EDAX) qualitative and quantitative elemental analysis. In primary cultures, the first stem cells emerged from the explants after 7 days (from tuberosity bone) and after 11 days (for the mandibular alveolar bone). The light microscopy examination revealed, in the developing stem cells cultures, spherical-oval masses, of varying size, with amorphous pearly white aspect and hyperchromatic centre. The scanning electron microscopy and EDAX qualitative and quantitative elemental analysis showed that these masses consisted of natrium chloride (NaCl) crystals precipitated around organic material. The masses of precipitated crystals progressively increased in size and number within the cell cultures, limiting the cell proliferation and development to reach confluence.Stem cells derived from human maxillary and mandibular bones promoted the precipitation of NaCl crystals in culture medium, which influenced the cells' development.

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Extracellular matrix mimicking scaffold promotes osteogenic stem cell differentiation: A new approach in osteoporosis research

Abstract: Overall, these results indicate that the use of HA-gelatine meshes as growth surfaces may serve as a valuable tool for cultivation and differentiation of mesenchymal stem cells along the osteogenic lineage, facilitating future research on osteoporosis and related issues.

Cited by 2 publications

References 42 publications

The Role of Chronic Inflammatory Bone and Joint Disorders in the Pathogenesis and Progression of Alzheimer's Disease

The Role of Chronic Inflammatory Bone and Joint Disorders in the Pathogenesis and Progression of Alzheimer's Disease

Interaction of stem cells derived from maxillary and mandibular bone with salts from culture medium

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