Extracellular Matrix of Retinal Pigment Epithelium Regulates Choriocapillaris Endothelial Survival in vitro

Liu, Xipu; Ye, Xiaodan; Yanoff, Myron; Li, Weiye

doi:10.1006/exer.1997.0317

Cited by 24 publications

(23 citation statements)

References 30 publications

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“…3,17 However, an alternative hypothesis could be that the loss of RPE and photoreceptors would cause choroidal thinning, by reduction of RPE produced trophic factors. 34,35 Additionally, beta-PPA was related to axial length in ours and previous studies. 14,16,17 Eyes with longer axial length might have thinner 36 or more atrophic RPE, more susceptible to degeneration.…”

Section: Discussionsupporting

confidence: 63%

Beta and Gamma Peripapillary Atrophy in Myopic Eyes With and Without Glaucoma

Vianna

Malik

Danthurebandara

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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PURPOSE. To determine whether beta and gamma peripapillary atrophy (PPA) areas measured with optical coherence tomography (OCT) enhances glaucoma diagnosis in myopic subjects.METHODS. We included 55 myopic glaucoma patients and 74 myopic nonglaucomatous controls. Beta-PPA comprised the area external to the clinical disc margin, with absence of retinal pigment epithelium and presence of Bruch's membrane. Gamma-PPA comprised the area external to the disc margin, with absence of both RPE and Bruch's membrane. OCT scans colocalized to fundus photographs were used to measure PPA, choroidal thickness, border tissue of Elschnig configuration, optic disc area, and optic disc ovality. 2 , respectively). However, the distributions of both beta-and gamma-PPA in the two groups overlapped widely. The areas under the receiver operating characteristic curve of beta-and gamma-PPA areas were 0.60 and 0.59, respectively. Larger beta-PPA area was associated with larger disc area, thinner choroidal thickness, longer axial length, less oblique border tissue configuration, older age, and greater disc ovality. Larger gamma-PPA area was associated with greater disc ovality, more oblique border tissue configuration, and longer axial length. RESULTS.CONCLUSIONS. Subclassifying PPA with OCT into beta and gamma zones reveals association with different covariates, but does not enhance the diagnostic performance for glaucoma in a population of predominantly Caucasians myopic subjects.

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Section: Discussionsupporting

confidence: 63%

Beta and Gamma Peripapillary Atrophy in Myopic Eyes With and Without Glaucoma

Vianna

Malik

Danthurebandara

et al. 2016

Invest. Ophthalmol. Vis. Sci.

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“…Cell Cultures CCE cultures were initiated as previously reported [16,17]. Briefly, the choroids from bovine eyes were minced, digested and agitated with 0.25% trypsin containing 2 mM EDTA, washed against a 50-Ìm-opening nylon mesh.…”

Section: Methodsmentioning

confidence: 99%

“…The resulting fragments of choriocapillaris were dispersed by collagenase (type I, Sigma Chemicals Co., St. Louis, Mo., USA; 1 mg/ml). The cells were seeded onto a fibronectin-coated surface in a modified endothelial growth medium (EGM) containing fetal calf serum (2%), bovine brain extract (4 Ìl/ml) and epidermal growth factor (10 ng/ml; Clonetics Co., San Diego, Calif., USA) [17]. CCE cells were further cloned after an incubation of 5-7 days and maintained in EGM.…”

Section: Methodsmentioning

confidence: 99%

“…To avoid the influence of the undefined components of serum and bovine brain extract in EGM for the experiments, CCE cells were incubated with an endothelial serum-free medium (endothelial SFM; Gibco BRL, Gaithersburg, Md., USA) for 24 h before being subcultured for the experiments. All the experiments were performed in a diluted endothelial SFM consisting of 50% endothelial SFM and 50% Locke's balanced salt solution, referred to as endo-SFM50 [17]. CCE cells at passages 5-10 were used for the present study, as previously reported [17].…”

Section: Methodsmentioning

confidence: 99%

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Regulatory Effects of Soluble Growth Factors on Choriocapillaris Endothelial Growth and Survival

Liu

Yanoff

et al. 1998

Ophthalmic Res

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The effects of soluble growth factors on regulating the survival and growth of choriocapillaris endothelial (CCE) cells were investigated in vitro. CCE cells were cultured in a serum-free medium in the presence or absence of various soluble growth factors. Cell growth and cell viability were assessed by counting viable cells. Results showed that acidic fibroblast growth factor (aFGF), basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF) significantly stimulated CCE growth in a dose-dependent manner. Platelet-derived growth factor BB (PDGF, 0.4–10 ng/ml), insulin-like growth factor (IGF, 0.4–10 ng/ml) or insulin (0.4–10 µg/ml) alone did not affect the growth of CCE cells. In the presence of insulin (10 µg/ml), however, PDGF stimulated CCE growth in a dose-dependent manner. By contrast, transforming growth factor β1 (TGF-β1) induced CCE death in the absence of other growth factors and inhibited the CCE growth induced by aFGF, bFGF or VEGF. When CCE cells had been cultured in media containing aFGF, bFGF, VEGF or the combination of PDGF and insulin for 48 h, withdrawal of aFGF, bFGF or VEGF, but not PDGF, from the media resulted in CCE death. The CCE death induced by either an introduction of TGF-β1 or withdrawal of aFGF, bFGF or VEGF was defined as apoptosis based on morphologic characteristics (condensation and fragmentation of nuclei, shrinkage of cells in size) and DNA fragmentation in multiples of approximately 180 base pairs. Phorbol 12-myristate 13-acetate (PMA, 2 or 5 nM) protected CCE cells against apoptosis induced by the introduction of TGF-β1 and withdrawal of aFGF, bFGF or VEGF, while H7 (50 µM), but not HA1004 (50 µM), abrogated the protective effect of PMA on CCE apoptosis. However, cycloheximide (0.1 µM), a protein synthesis inhibitor, was only effective to protect CCE cells from apoptosis induced by aFGF, bFGF or VEGF withdrawal, but not that induced by the introduction of TGF-β1. Moreover, cycloheximide and a low concentration of PMA (2 nM) showed an additive effect on protecting CCE cells from apoptosis as the result of a growth factor withdrawal. These data may suggest that some growth factors, such as aFGF, bFGF, VEGF and TGF-β1, play a more critical role than the others, such as PDGF and IGF, in the regulation of CCE growth and survival. CCE apoptosis as a result of withdrawal of a survival factor or of receiving a death factor such as TGF-β1 may involve different mechanisms, which merits further study.

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“…In the case of in vitro endothelialization, the ECM could effectively attenuate the shedding of seeded endothelial cells (ECs) in the bloodstream by shear stress, 1 help to maintain their physiological phenotype, 2,3 and even prevent them from undergoing apoptosis. 4 In terms of in vivo endothelialization, the ECM was essential for cell adhesion and migration via integrin receptors. 5 Furthermore, it could direct endothelial differentiation of endothelial progenitor cells or marrow stem cells.…”

Section: Introductionmentioning

confidence: 99%

Effect of Tissue Specificity on the Performance of Extracellular Matrix in Improving Endothelialization of Cardiovascular Implants

Yang

Zhu

et al. 2013

Tissue Engineering Part A

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Natural extracellular matrix (ECM) deposited in situ by cultured endothelial cells (ECs) has been proven effective in accelerating endothelialization of titanium (Ti) cardiovascular implants (CVIs) in our previous studies. In this study, the ECM deposited by smooth muscle cells (SMCs) was used in comparison to investigate the effects of tissue specificity of the ECM on the ability to accelerate endothelialization of CVIs. The results demonstrated that the ECM deposited by ECs and SMCs (EC-ECM, SMC-ECM, respectively) differed considerably in components and fibril morphology. Surface modification of Ti CVIs with both types of natural ECM was effective in improving their in vitro hemocompatibility and cytocompatibility simultaneously. However, the endothelialization of ECM-modified Ti CVIs in a canine model demonstrated a high tissue specificity of the ECM. Although the ECM deposited by SMCs (SMC-ECM) induced fewer platelet adhesion and sustained better growth and viability of ECs in vitro, its performance in accelerating in vivo endothelialization of Ti CVIs was extremely poor. In contrast, the ECM deposited by ECs (EC-ECM) led to complete endothelium formation in vivo.

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Extracellular Matrix of Retinal Pigment Epithelium Regulates Choriocapillaris Endothelial Survival in vitro

Cited by 24 publications

References 30 publications

Beta and Gamma Peripapillary Atrophy in Myopic Eyes With and Without Glaucoma

Beta and Gamma Peripapillary Atrophy in Myopic Eyes With and Without Glaucoma

Regulatory Effects of Soluble Growth Factors on Choriocapillaris Endothelial Growth and Survival

Effect of Tissue Specificity on the Performance of Extracellular Matrix in Improving Endothelialization of Cardiovascular Implants

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